Restriction map for
pFN21AB7673
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BciVI BclI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HindIII
HinfI HpaI HphI KasI KpnI
MaeII MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AgeI 7 ACCGGT A^CCGGT
AlwI 622 GATCC GGATC(4/5)
Asp700I 659 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 391 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 175 GGCACC G^G[CT][GA]CC
BcgI 26 GCAGCGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 32 GCCTCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 819 AGATCT A^GATCT
BpmI 58 CTGGAG CTGGAG(16/14)
BsaI 935 GAGACC GGTCTC(1/5)
BsgI 356 CTGCAC GTGCAG(16/14)
BspMI 240 ACCTGC ACCTGC(4/8)
BsrBI 556 GAGCGG CCGCTC(-3/-3)
BstXI 485 CCACCGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 420 GGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 185 CGGCCG [CT]^GGCC[GA]
EagI 185 CGGCCG C^GGCCG
EarI 456 GAAGAG CTCTTC(1/4)
EclXI 185 CGGCCG C^GGCCG
Eco52I 185 CGGCCG C^GGCCG
EcoO109I 420 GGGCCCC [GA]G^G[ACGT]CC[CT]
HaeII 479 GGCGCT [GA]GCGC^[CT]
HincII 496 GTCAAC GT[CT]^[GA]AC
HindII 496 GTCAAC GT[CT]^[GA]AC
Ksp632I 456 GAAGAG CTCTTC(1/4)
MaeIII 374 GTGAC ^GT[ACGT]AC
MseI 928 TTAA T^TAA
MslI 486 CACCGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII 288 CATG CATG^
NotI 184 GCGGCCGC GC^GGCCGC
PflFI 391 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 7 ACCGGT A^CCGGT
PvuII 1009 CAGCTG CAG^CTG
SfcI 643 CTACAG C^T[GA][CT]AG
SgrAI 486 CACCGGCG C[GA]^CCGG[CT]G
SmlI 711 CTCAAG C^T[CT][GA]AG
Tru9I 928 TTAA T^TAA
Tsp45I 374 GTGAC ^GT[GC]AC
Tth111I 391 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 47 CCACAGACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 659 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 142 GACGCC G[GA]^CG[CT]C
394 GGCGTC
AfaI 528 GTAC GT^AC
779 GTAC
ApaI 111 GGGCCC GGGCC^C
420 GGGCCC
AspHI 202 GAGCTC G[AT]GC[AT]^C
414 GAGCTC
AvaII 20 GGACC G^G[AT]CC
253 GGTCC
BsaHI 142 GACGCC G[GA]^CG[CT]C
394 GGCGTC
BsaWI 7 ACCGGT [AT]^CCGG[AT]
17 ACCGGA
BsiHKAI 202 GAGCTC G[AT]GC[AT]^C
414 GAGCTC
BsrFI 7 ACCGGT [GA]^CCGG[CT]
487 ACCGGC
BssHII 90 GCGCGC G^CGCGC
227 GCGCGC
BstF5I 501 CATCC GGATG(2/0)
762 GGATG
BstYI 621 AGATCC [GA]^GATC[CT]
819 AGATCT
Cfr10I 7 ACCGGT [GA]^CCGG[CT]
487 ACCGGC
DdeI 519 CTTAG C^T[ACGT]AG
859 CTAAG
DsaI 161 CCGCGG C^C[GA][CT]GG
313 CCGCGG
FokI 501 CATCC GGATG(9/13)
762 GGATG
Hin1I 142 GACGCC G[GA]^CG[CT]C
394 GGCGTC
KspI 161 CCGCGG CCGC^GG
313 CCGCGG
MflI 621 AGATCC [GA]^GATC[CT]
819 AGATCT
RsaI 528 GTAC GT^AC
779 GTAC
SacI 202 GAGCTC GAGCT^C
414 GAGCTC
SacII 161 CCGCGG CCGC^GG
313 CCGCGG
TaqI 452 TCGA T^CGA
726 TCGA
TthHB8I 452 TCGA T^CGA
726 TCGA
XhoII 621 AGATCC [GA]^GATC[CT]
819 AGATCT
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AluI AvaI BanII
BbvI BcnI BfaI BmyI BsaJI
BseRI BsiEI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DpnII EcoRII FauI Fnu4HI HaeIII
HapII HgaI HhaI HinP1I HpaII
ItaI MaeI MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIV Sau3AI Sau96I
ScrFI SfaNI SmaI TseI Tsp509I
TspRI XmaCI XmaI