##################################
# NO CUT ENZYMES (  188 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BseAI       BseRI       BsgI        
BsiHKAI     BsiWI       BsiYI       BslI        BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr13I      ClaI        CpoI        
DpnII       DraI        DraII       DrdI        DsaI        
Eam1105I    Eco47III    Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeII       MaeIII      MamI        MboI        MfeI        
MflI        MluI        MroI        MseI        MslI        
MspA1I      MunI        NaeI        NarI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NlaIII      NlaIV       NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      Sau96I      SbfI        
ScaI        SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        TfiI        
Tru9I       Tsp45I      Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AgeI          118  ACCGGT               A^CCGGT
AluI          189  AGCT                 AG^CT
AvrII         238  CCTAGG               C^CTAGG
BalI          247  TGGCCA               TGG^CCA
BanII         124  GAGCCC               G[GA]GC[CT]^C
BbsI          149  GAAGAC               GAAGAC(2/6)
BfaI          239  CTAG                 C^TAG
BlnI          238  CCTAGG               C^CTAGG
BmyI          124  GAGCCC               G[AGT]GC[ACT]^C
BpmI          212  CTGGAG               CTGGAG(16/14)
BpuAI         149  GAAGAC               GAAGAC(2/6)
BsaI          138  GAGACC               GGTCTC(1/5)
BsaWI         118  ACCGGT               [AT]^CCGG[AT]
BsiEI          87  CGGCCG               CG[GA][CT]^CG
Bsp1286I      124  GAGCCC               G[AGT]GC[ACT]^C
BspMI          54  ACCTGC               ACCTGC(4/8)
BsrFI         118  ACCGGT               [GA]^CCGG[CT]
BsrI          211  ACTGG                ACTGG(1/-1)
BstF5I        299  CATCC                GGATG(2/0)
Cfr10I        118  ACCGGT               [GA]^CCGG[CT]
DraIII          8  CACGCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI           87  CGGCCG               C^GGCCG
EclXI          87  CGGCCG               C^GGCCG
Eco52I         87  CGGCCG               C^GGCCG
EcoT14I       238  CCTAGG               C^C[AT][AT]GG
FauI           22  GCGGG                CCCGC(4/6)
FokI          299  CATCC                GGATG(9/13)
HaeII          66  AGCGCC               [GA]GCGC^[CT]
HapII         119  CCGG                 C^CGG
HgaI           83  GACGC                GACGC(5/10)
HpaII         119  CCGG                 C^CGG
MaeI          239  CTAG                 C^TAG
MluNI         247  TGGCCA               TGG^CCA
MscI          247  TGGCCA               TGG^CCA
MspI          119  CCGG                 C^CGG
NotI           86  GCGGCCGC             GC^GGCCGC
PinAI         118  ACCGGT               A^CCGGT
StyI          238  CCTAGG               C^C[AT][AT]GG
TspRI         210  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

BsmAI         138  GAGAC                GTCTC(1/5)
              295  GAGAC               
BsmFI          48  GGGAC                GGGAC(10/14)
              176  GTCCC               
Cac8I           7  GCACGC               GC[ACGT]^[ACGT]GC
              273  GCCTGC              
HinfI         174  GAGTC                G^A[ACGT]TC
              310  GAGTC               
HphI           13  GGTGA                GGTGA(8/7)
              121  GGTGA               
MwoI           11  GCGGTGAGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               58  GCCGCCCCAGC         
PleI          174  GAGTC                GAGTC(4/5)
              310  GAGTC               


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AccII       AciI        BbvI        BsaJI       BssKI       
BstNI       BstUI       CfoI        DdeI        EaeI        
EarI        EcoRII      Fnu4HI      HaeIII      HhaI        
HinP1I      ItaI        Ksp632I     MboII       MnlI        
MvaI        MvnI        ScrFI       TseI