Restriction map for
pFN21AE5978
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# NO CUT ENZYMES ( 182 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BanI BanII BbeI BbrPI
BbsI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII BssKI BssSI
Bst1107I BstBI BstEII BstNI BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraII DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoRV
EcoT14I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MamI
MfeI MluI MroI MspI MunI
MvaI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI ScrFI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tth111I TthHB8I Van91I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AflIII 98 ACATGT A^C[GA][CT]GT
AlwNI 64 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 365 TGGCCA TGG^CCA
BamHI 147 GGATCC G^GATCC
BbvCI 55 CCTCAGC CCTCAGC(-5/-2)
BfaI 490 CTAG C^TAG
BsiYI 309 CCTCTTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 309 CCTCTTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 39 GGGAC GGGAC(10/14)
BspLU11I 98 ACATGT A^CATGT
BspMI 63 GCAGGT ACCTGC(4/8)
BsrDI 536 CATTGC GCAATG(2/0)
BsrI 253 ACTGG ACTGG(1/-1)
BstAPI 63 GCAGGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 927 TTTAAA TTT^AAA
DrdI 502 GACTCTAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 365 TGGCCA [CT]^GGCC[GA]
EarI 790 GAAGAG CTCTTC(1/4)
HaeIII 366 GGCC GG^CC
Ksp632I 790 GAAGAG CTCTTC(1/4)
MaeI 490 CTAG C^TAG
MaeII 271 ACGT A^CGT
MluNI 365 TGGCCA TGG^CCA
MscI 365 TGGCCA TGG^CCA
MslI 93 CACCTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 63 GCAGGTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 350 CATATG CA^TATG
NlaIV 147 GGATCC GG[ACGT]^[ACGT]CC
PstI 61 CTGCAG CTGCA^G
SfcI 61 CTGCAG C^T[GA][CT]AG
TfiI 233 GATTC G^A[AT]TC
Tsp45I 500 GTGAC ^GT[GC]AC
TspRI 818 CAGTG CA[GC]TG[ACGT][ACGT]^
XbaI 489 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AseI 573 ATTAAT AT^TAAT
802 ATTAAT
AsnI 573 ATTAAT AT^TAAT
802 ATTAAT
Asp700I 325 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
849 GAATTCTTTC
BspHI 398 TCATGA T^CATGA
897 TCATGA
BstYI 51 AGATCC [GA]^GATC[CT]
147 GGATCC
EcoRI 493 GAATTC G^AATTC
849 GAATTC
EcoT22I 278 ATGCAT ATGCA^T
533 ATGCAT
MflI 51 AGATCC [GA]^GATC[CT]
147 GGATCC
MspA1I 58 CAGCTG C[AC]G^C[GT]G
753 CAGCTG
NsiI 278 ATGCAT ATGCA^T
533 ATGCAT
NspI 98 ACATGT [GA]CATG^[CT]
280 GCATGT
PleI 502 GACTC GAGTC(4/5)
901 GACTC
PshBI 573 ATTAAT AT^TAAT
802 ATTAAT
PvuII 58 CAGCTG CAG^CTG
753 CAGCTG
RcaI 398 TCATGA T^CATGA
897 TCATGA
SfaNI 532 GATGC GCATC(5/9)
758 GCATC
XhoII 51 AGATCC [GA]^GATC[CT]
147 GGATCC
XmnI 325 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
849 GAATTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BstF5I DdeI DpnII Fnu4HI FokI
HinfI HphI ItaI MaeIII MboI
MboII MnlI MseI NdeII NlaIII
Sau3AI Tru9I TseI Tsp509I