Restriction map for
pFN21AE5957
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# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AvaII AviII AvrII BamHI
BanI BbeI BbrPI BcgI BciVI
BcnI BfrI BglI BglII BlnI
BpmI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsiYI BslI
BsmBI BsoBI Bsp1407I BspDI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MamI MfeI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI Sau96I SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tth111I
TthHB8I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AciI 55 CCGC CCGC(-3/-1)
AflIII 720 ACATGT A^C[GA][CT]GT
AlwI 407 GGATC GGATC(4/5)
AlwNI 405 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 180 CCTCAGG CC^T[ACGT]AGG
Asp700I 908 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 577 GAGCTC G[AT]GC[AT]^C
BalI 470 TGGCCA TGG^CCA
BanII 577 GAGCTC G[GA]GC[CT]^C
BbsI 159 GAAGAC GAAGAC(2/6)
BbvCI 677 CCTCAGC CCTCAGC(-5/-2)
BclI 236 TGATCA T^GATCA
BlpI 286 GCTCAGC GC^T[ACGT]AGC
BmyI 577 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 286 GCTCAGC GC^T[ACGT]AGC
BpuAI 159 GAAGAC GAAGAC(2/6)
BsaAI 93 TACGTA [CT]AC^GT[GA]
BseRI 714 CTCCTC GAGGAG(10/8)
BsgI 281 GTGCAG GTGCAG(16/14)
BsiHKAI 577 GAGCTC G[AT]GC[AT]^C
BsmAI 733 GTCTC GTCTC(1/5)
BsmFI 254 GGGAC GGGAC(10/14)
BsmI 242 GCATTC GAATGC(1/-1)
Bsp1286I 577 GAGCTC G[AGT]GC[ACT]^C
BspLU11I 720 ACATGT A^CATGT
BssKI 333 CCTGG ^CC[ACGT]GG
BstNI 333 CCTGG CC^[AT]GG
BstYI 407 GGATCT [GA]^GATC[CT]
Bsu36I 180 CCTCAGG CC^T[ACGT]AGG
Cac8I 438 GCTGGC GC[ACGT]^[ACGT]GC
CelII 286 GCTCAGC GC^T[ACGT]AGC
DrdI 499 GACTCCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 473 CCATGG C^C[GA][CT]GG
EaeI 470 TGGCCA [CT]^GGCC[GA]
Eco81I 180 CCTCAGG CC^T[ACGT]AGG
EcoRII 333 CCTGG ^CC[AT]GG
FbaI 236 TGATCA T^GATCA
HphI 879 GGTGA GGTGA(8/7)
MaeII 94 ACGT A^CGT
MflI 407 GGATCT [GA]^GATC[CT]
MluNI 470 TGGCCA TGG^CCA
MscI 470 TGGCCA TGG^CCA
MseI 893 TTAA T^TAA
MspA1I 484 CAGCTG C[AC]G^C[GT]G
MvaI 333 CCTGG CC^[AT]GG
MwoI 277 GCTTGTGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 473 CCATGG C^CATGG
NspI 720 ACATGT [GA]CATG^[CT]
PstI 61 CTGCAG CTGCA^G
PvuII 484 CAGCTG CAG^CTG
SacI 577 GAGCTC GAGCT^C
ScaI 568 AGTACT AGT^ACT
ScrFI 333 CCTGG CC^[ACGT]GG
SfaNI 755 GCATC GCATC(5/9)
SfcI 61 CTGCAG C^T[GA][CT]AG
SnaBI 93 TACGTA TAC^GTA
Tru9I 893 TTAA T^TAA
XbaI 573 TCTAGA T^CTAGA
XhoII 407 GGATCT [GA]^GATC[CT]
XmnI 908 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AcsI 186 GAATTT [GA]^AATT[CT]
200 AAATTT
AfaI 100 GTAC GT^AC
569 GTAC
ApoI 186 GAATTT [GA]^AATT[CT]
200 AAATTT
BfaI 227 CTAG C^TAG
574 CTAG
BspHI 395 TCATGA T^CATGA
581 TCATGA
BsrI 112 ACTGG ACTGG(1/-1)
468 ACTGG
BstF5I 30 CATCC GGATG(2/0)
897 GGATG
DpnII 237 GATC ^GATC
408 GATC
FokI 30 CATCC GGATG(9/13)
897 GGATG
HaeIII 336 GGCC GG^CC
471 GGCC
MaeI 227 CTAG C^TAG
574 CTAG
MboI 237 GATC ^GATC
408 GATC
MslI 756 CATCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
762 CATGTATGTG
NdeII 237 GATC ^GATC
408 GATC
PleI 499 GACTC GAGTC(4/5)
529 GACTC
RcaI 395 TCATGA T^CATGA
581 TCATGA
RsaI 100 GTAC GT^AC
569 GTAC
Sau3AI 237 GATC ^GATC
408 GATC
TspRI 17 CAGTG CA[GC]TG[ACGT][ACGT]^
467 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI BbvI BsaJI BspMI DdeI
EcoT14I Fnu4HI HinfI ItaI MaeIII
MboII MnlI NlaIII StyI TfiI
TseI Tsp45I Tsp509I