Restriction map for
pFN21AE5963
##################################
# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BsgI BsiEI
BsiWI BsmBI BsmFI BsoBI BspDI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRI EcoRV
EcoT14I EcoT22I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MluI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI TaqI Tth111I
TthHB8I Van91I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 659 TCCGGA T^CCGGA
AciI 357 CCGC CCGC(-3/-1)
AflIII 98 ACATGT A^C[GA][CT]GT
AlwI 148 GATCC GGATC(4/5)
AlwNI 750 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 906 GGTCC G^G[AT]CC
BalI 365 TGGCCA TGG^CCA
BfaI 577 CTAG C^TAG
BlpI 289 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 289 GCTCAGC GC^T[ACGT]AGC
BsaWI 659 TCCGGA [AT]^CCGG[AT]
BseAI 659 TCCGGA T^CCGGA
BsmAI 736 GTCTC GTCTC(1/5)
BsmI 224 GCATTC GAATGC(1/-1)
Bsp1407I 767 TGTACA T^GTACA
BspEI 659 TCCGGA T^CCGGA
BspHI 398 TCATGA T^CATGA
BspLU11I 98 ACATGT A^CATGT
BspMI 63 GCAGGT ACCTGC(4/8)
BsrGI 767 TGTACA T^GTACA
BstEII 618 GGTCACC G^GT[ACGT]ACC
BstF5I 657 CATCC GGATG(2/0)
BstPI 618 GGTCACC G^GT[ACGT]ACC
BstYI 147 AGATCC [GA]^GATC[CT]
CelII 289 GCTCAGC GC^T[ACGT]AGC
Cfr13I 906 GGTCC G^G[ACGT]CC
DraI 927 TTTAAA TTT^AAA
EaeI 365 TGGCCA [CT]^GGCC[GA]
EcoO65I 618 GGTCACC G^GT[ACGT]ACC
FokI 657 CATCC GGATG(9/13)
HaeIII 366 GGCC GG^CC
HapII 660 CCGG C^CGG
HpaII 660 CCGG C^CGG
MaeI 577 CTAG C^TAG
MaeII 214 ACGT A^CGT
MflI 147 AGATCC [GA]^GATC[CT]
MluNI 365 TGGCCA TGG^CCA
MroI 659 TCCGGA T^CCGGA
MscI 365 TGGCCA TGG^CCA
MspA1I 357 CCGCTG C[AC]G^C[GT]G
MspI 660 CCGG C^CGG
NdeI 724 CATATG CA^TATG
NspI 98 ACATGT [GA]CATG^[CT]
RcaI 398 TCATGA T^CATGA
Sau96I 906 GGTCC G^G[ACGT]CC
SspBI 767 TGTACA T^GTACA
XbaI 576 TCTAGA T^CTAGA
XhoII 147 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Asp700I 263 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
849 GAATCCCTTC
AspHI 402 GAGCAC G[AT]GC[AT]^C
820 GTGCTC
BmyI 402 GAGCAC G[AGT]GC[ACT]^C
820 GTGCTC
BsaBI 123 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
148 GATCCCCATC
BseRI 717 CTCCTC GAGGAG(10/8)
839 CTCCTC
BsiHKAI 402 GAGCAC G[AT]GC[AT]^C
820 GTGCTC
BsiYI 357 CCGCTGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
785 CCAGAGAAAGG
BslI 357 CCGCTGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
785 CCAGAGAAAGG
Bsp1286I 402 GAGCAC G[AGT]GC[ACT]^C
820 GTGCTC
BsrI 112 ACTGG ACTGG(1/-1)
469 CCAGT
BssKI 198 CCTGG ^CC[ACGT]GG
437 CCTGG
BstNI 198 CCTGG CC^[AT]GG
437 CCTGG
Cac8I 441 GCTTGC GC[ACGT]^[ACGT]GC
888 GCAAGC
EarI 380 CTCTTC CTCTTC(1/4)
534 CTCTTC
EcoRII 198 CCTGG ^CC[AT]GG
437 CCTGG
Ksp632I 380 CTCTTC CTCTTC(1/4)
534 CTCTTC
MamI 123 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
148 GATCCCCATC
MslI 243 CACTGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
405 CACCAGAGTG
MvaI 198 CCTGG CC^[AT]GG
437 CCTGG
MwoI 477 GCTTCTGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
879 GCAAGTGAAGC
NlaIV 116 GGAACC GG[ACGT]^[ACGT]CC
837 GGCTCC
ScrFI 198 CCTGG CC^[ACGT]GG
437 CCTGG
TfiI 233 GATTC G^A[AT]TC
849 GAATC
XmnI 263 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
849 GAATCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BclI DdeI DpnII FbaI Fnu4HI
HinfI HphI ItaI MaeIII MboI
MboII MnlI MseI NdeII NlaIII
PleI PstI RsaI Sau3AI SfcI
Tru9I TseI Tsp45I Tsp509I TspRI