Restriction map for
pFN21AE5973
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BamHI BanI BanII BbeI BbrPI
BciVI BclI BcnI BfrI BglI
BglII BlnI BmyI BpmI BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspDI BspEI BsrBI BsrDI
BsrFI BssHII BssKI BssSI BstAPI
BstBI BstEII BstF5I BstNI BstPI
BstUI BstYI Bsu36I CfoI Cfr10I
Cfr13I ClaI CpoI DpnII DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoRV EcoT14I
EcoT22I FauI FbaI FokI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
HpaII KasI KpnI KspI MaeII
MamI MboI MfeI MflI MluI
MroI MspI MunI MvaI MvnI
NaeI NarI NciI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SapI Sau3AI
Sau96I SbfI ScaI ScrFI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 224 GTATAC GT^[AC][GT]AC
AciI 357 CCGC CCGC(-3/-1)
AfaI 768 GTAC GT^AC
AflIII 712 ACATGT A^C[GA][CT]GT
AseI 630 ATTAAT AT^TAAT
AsnI 630 ATTAAT AT^TAAT
Asp700I 674 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 365 TGGCCA TGG^CCA
BbvCI 779 CCTCAGC CCTCAGC(-5/-2)
BcgI 406 CGAAGAGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 686 CTAG C^TAG
BlpI 102 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 102 GCTCAGC GC^T[ACGT]AGC
BsgI 414 CTGCAC GTGCAG(16/14)
BsiYI 357 CCGCTATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 357 CCGCTATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1407I 767 TGTACA T^GTACA
BspHI 398 TCATGA T^CATGA
BspLU11I 712 ACATGT A^CATGT
BspMI 155 ACCTGC ACCTGC(4/8)
BsrGI 767 TGTACA T^GTACA
BsrI 112 ACTGG ACTGG(1/-1)
Bst1107I 224 GTATAC GTA^TAC
BstXI 230 CCAAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 224 GTATAC GTA^TAC
CelII 102 GCTCAGC GC^T[ACGT]AGC
DraI 483 TTTAAA TTT^AAA
EaeI 365 TGGCCA [CT]^GGCC[GA]
EarI 407 GAAGAG CTCTTC(1/4)
EcoRI 674 GAATTC G^AATTC
Ksp632I 407 GAAGAG CTCTTC(1/4)
MaeI 686 CTAG C^TAG
MluNI 365 TGGCCA TGG^CCA
MscI 365 TGGCCA TGG^CCA
MspA1I 753 CAGCTG C[AC]G^C[GT]G
NlaIV 116 GGAACC GG[ACGT]^[ACGT]CC
PshBI 630 ATTAAT AT^TAAT
PstI 157 CTGCAG CTGCA^G
PvuII 753 CAGCTG CAG^CTG
RcaI 398 TCATGA T^CATGA
RsaI 768 GTAC GT^AC
SfaNI 107 GCATC GCATC(5/9)
SpeI 685 ACTAGT A^CTAGT
SspBI 767 TGTACA T^GTACA
StuI 701 AGGCCT AGG^CCT
TaqI 405 TCGA T^CGA
Tsp45I 319 GTCAC ^GT[GC]AC
TthHB8I 405 TCGA T^CGA
XmnI 674 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
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BbsI 912 GAAGAC GAAGAC(2/6)
79 GTCTTC
BpuAI 912 GAAGAC GAAGAC(2/6)
79 GTCTTC
Cac8I 374 GCAAGC GC[ACGT]^[ACGT]GC
888 GCAAGC
HaeIII 366 GGCC GG^CC
702 GGCC
HindIII 487 AAGCTT A^AGCTT
890 AAGCTT
HphI 582 GGTGA GGTGA(8/7)
92 TCACC
MaeIII 242 GTAAC ^GT[ACGT]AC
319 GTCAC
MwoI 480 GCTTTTAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
879 GCAAGTCAAGC
NspI 98 ACATGC [GA]CATG^[CT]
712 ACATGT
PleI 317 GAGTC GAGTC(4/5)
410 GAGTC
SfcI 157 CTGCAG C^T[GA][CT]AG
498 CTGTAG
SmlI 390 CTTGAG C^T[CT][GA]AG
801 CTTGAG
SspI 508 AATATT AAT^ATT
633 AATATT
TfiI 148 GATTC G^A[AT]TC
402 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AcsI AluI ApoI BbvI DdeI
Fnu4HI HinfI ItaI MboII MnlI
MseI MslI NlaIII Tru9I TseI
Tsp509I TspRI