Restriction map for
pFN21AE5482
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaII AviII BalI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BcnI BfrI BglI BglII
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsiYI BslI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoT22I FauI FseI FspI HaeII
HapII HgaI Hin1I HindIII HpaI
HpaII KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MseI MspA1I MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SwaI
TaqI Tru9I TspRI Tth111I TthHB8I
Van91I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 976 AAATTT [GA]^AATT[CT]
AlwNI 628 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 976 AAATTT [GA]^AATT[CT]
Asp700I 780 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 139 GAGCTC G[AT]GC[AT]^C
AvaI 587 CTCGGG C^[CT]CG[GA]G
AvrII 261 CCTAGG C^CTAGG
BamHI 7 GGATCC G^GATCC
BanII 139 GAGCTC G[GA]GC[CT]^C
BclI 320 TGATCA T^GATCA
BlnI 261 CCTAGG C^CTAGG
BmyI 139 GAGCTC G[AGT]GC[ACT]^C
BsiHKAI 139 GAGCTC G[AT]GC[AT]^C
BsmAI 630 GAGAC GTCTC(1/5)
BsmFI 771 GGGAC GGGAC(10/14)
BsoBI 587 CTCGGG C^[CT]CG[GA]G
Bsp1286I 139 GAGCTC G[AGT]GC[ACT]^C
BspLU11I 728 ACATGT A^CATGT
BspMI 793 ACCTGC ACCTGC(4/8)
BstYI 7 GGATCC [GA]^GATC[CT]
CfoI 581 GCGC GCG^C
Cfr13I 369 GGCCC G^G[ACGT]CC
EaeI 422 CGGCCA [CT]^GGCC[GA]
EarI 154 CTCTTC CTCTTC(1/4)
EcoRV 666 GATATC GAT^ATC
FbaI 320 TGATCA T^GATCA
HhaI 581 GCGC GCG^C
HinP1I 581 GCGC G^CGC
HincII 595 GTTGAC GT[CT]^[GA]AC
HindII 595 GTTGAC GT[CT]^[GA]AC
Ksp632I 154 CTCTTC CTCTTC(1/4)
MaeII 489 ACGT A^CGT
MflI 7 GGATCC [GA]^GATC[CT]
NspI 728 ACATGT [GA]CATG^[CT]
PstI 795 CTGCAG CTGCA^G
SacI 139 GAGCTC GAGCT^C
Sau96I 369 GGCCC G^G[ACGT]CC
SfaNI 889 GCATC GCATC(5/9)
SfcI 795 CTGCAG C^T[GA][CT]AG
SspI 994 AATATT AAT^ATT
StuI 782 AGGCCT AGG^CCT
Tsp509I 977 AATT ^AATT
XbaI 981 TCTAGA T^CTAGA
XhoII 7 GGATCC [GA]^GATC[CT]
XmnI 780 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 438 CCGC CCGC(-3/-1)
421 GCGG
AfaI 130 GTAC GT^AC
497 GTAC
AflIII 487 ACACGT A^C[GA][CT]GT
728 ACATGT
BbvI 797 GCAGC GCAGC(8/12)
530 GCTGC
BseRI 151 CTCCTC GAGGAG(10/8)
391 CTCCTC
BssKI 417 CCTGG ^CC[ACGT]GG
807 CCTGG
BstF5I 890 CATCC GGATG(2/0)
206 GGATG
BstNI 417 CCTGG CC^[AT]GG
807 CCTGG
Cac8I 118 GCAGGC GC[ACGT]^[ACGT]GC
358 GCAGGC
DsaI 65 CCATGG C^C[GA][CT]GG
425 CCATGG
EcoRII 417 CCTGG ^CC[AT]GG
807 CCTGG
FokI 890 CATCC GGATG(9/13)
206 GGATG
HinfI 613 GATTC G^A[ACGT]TC
924 GAATC
HphI 70 GGTGA GGTGA(8/7)
804 TCACC
MboII 987 GAAGA GAAGA(8/7)
155 TCTTC
MvaI 417 CCTGG CC^[AT]GG
807 CCTGG
NcoI 65 CCATGG C^CATGG
425 CCATGG
NlaIV 7 GGATCC GG[ACGT]^[ACGT]CC
514 GGTTCC
RsaI 130 GTAC GT^AC
497 GTAC
ScrFI 417 CCTGG CC^[ACGT]GG
807 CCTGG
TfiI 613 GATTC G^A[AT]TC
924 GAATC
TseI 530 GCTGC G^C[AT]GC
797 GCAGC
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AluI AlwI BfaI BsaJI DdeI
DpnII EcoT14I Fnu4HI HaeIII ItaI
MaeI MaeIII MboI MnlI MslI
MwoI NdeII NlaIII Sau3AI StyI
Tsp45I