Restriction map for
pFN21AB9895
##################################
# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AflIII AgeI
AocI Aor51HI AscI AseI AsnI
Asp718I AspI AvaI AvaII AviII
AvrII BbrPI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmAI BsmBI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HapII HgaI
HindIII HinfI HpaI HpaII KpnI
KspI MaeI MaeII MamI MfeI
MluI MroI MspA1I MspI MunI
MvnI NaeI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tth111I
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AciI 121 GCGG CCGC(-3/-1)
AcsI 43 AAATTT [GA]^AATT[CT]
AcyI 407 GGCGCC G[GA]^CG[CT]C
AhdI 530 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 392 GTGCAC G^TGCAC
AlwNI 73 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 297 GGGCCC GGGCC^C
ApaLI 392 GTGCAC G^TGCAC
ApoI 43 AAATTT [GA]^AATT[CT]
Asp700I 480 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 530 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 392 GTGCAC G[AT]GC[AT]^C
BalI 674 TGGCCA TGG^CCA
BamHI 493 GGATCC G^GATCC
BanI 407 GGCGCC G^G[CT][GA]CC
BbeI 407 GGCGCC GGCGC^C
BpmI 307 CTGGAG CTGGAG(16/14)
BsaHI 407 GGCGCC G[GA]^CG[CT]C
BsiHKAI 392 GTGCAC G[AT]GC[AT]^C
BsmI 274 GCATTC GAATGC(1/-1)
BspHI 662 TCATGA T^CATGA
BspMI 376 ACCTGC ACCTGC(4/8)
BsrDI 364 GCAATG GCAATG(2/0)
BstF5I 336 CATCC GGATG(2/0)
BstYI 493 GGATCC [GA]^GATC[CT]
DraI 39 TTTAAA TTT^AAA
EaeI 674 TGGCCA [CT]^GGCC[GA]
Eam1105I 530 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 628 CTCTTC CTCTTC(1/4)
EcoNI 78 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 182 CCTTGG C^C[AT][AT]GG
FokI 336 CATCC GGATG(9/13)
HaeII 407 GGCGCC [GA]GCGC^[CT]
Hin1I 407 GGCGCC G[GA]^CG[CT]C
HphI 383 TCACC GGTGA(8/7)
KasI 407 GGCGCC G^GCGCC
Ksp632I 628 CTCTTC CTCTTC(1/4)
MaeIII 148 GTCAC ^GT[ACGT]AC
MflI 493 GGATCC [GA]^GATC[CT]
MluNI 674 TGGCCA TGG^CCA
MscI 674 TGGCCA TGG^CCA
MseI 40 TTAA T^TAA
NarI 407 GGCGCC GG^CGCC
NdeI 142 CATATG CA^TATG
PstI 125 CTGCAG CTGCA^G
RcaI 662 TCATGA T^CATGA
SfcI 125 CTGCAG C^T[GA][CT]AG
SfoI 407 GGCGCC GGC^GCC
StyI 182 CCTTGG C^C[AT][AT]GG
TaqI 15 TCGA T^CGA
Tru9I 40 TTAA T^TAA
Tsp45I 148 GTCAC ^GT[GC]AC
Tsp509I 44 AATT ^AATT
TthHB8I 15 TCGA T^CGA
XcmI 344 CCACTGCTTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 493 GGATCC [GA]^GATC[CT]
XmnI 480 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AluI 415 AGCT AG^CT
454 AGCT
BanII 186 GGGCTC G[GA]GC[CT]^C
297 GGGCCC
BbsI 468 GTCTTC GAAGAC(2/6)
649 GTCTTC
BpuAI 468 GTCTTC GAAGAC(2/6)
649 GTCTTC
BsmFI 255 GGGAC GGGAC(10/14)
528 GGGAC
BsrI 252 ACTGG ACTGG(1/-1)
531 ACTGG
BstAPI 94 GCAAGTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
394 GCACTCTATGC
Cac8I 166 GCTGGC GC[ACGT]^[ACGT]GC
170 GCCTGC
CfoI 34 GCGC GCG^C
408 GCGC
DraII 130 GGGGCCT [GA]G^G[ACGT]CC[CT]
297 GGGCCCT
EcoO109I 130 GGGGCCT [GA]G^G[ACGT]CC[CT]
297 GGGCCCT
HhaI 34 GCGC GCG^C
408 GCGC
HinP1I 34 GCGC G^CGC
408 GCGC
HincII 63 GTCAAC GT[CT]^[GA]AC
325 GTCAAC
HindII 63 GTCAAC GT[CT]^[GA]AC
325 GTCAAC
MslI 226 CATAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
360 CACGGCAATG
SfaNI 31 GATGC GCATC(5/9)
335 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AlwI BbvI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstNI Cfr13I
DdeI DpnII EcoRII Fnu4HI HaeIII
ItaI MboI MboII MnlI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TseI TspRI