Restriction map for
pFN21AE5079
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccIII AclI AfaI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaII AvrII
BalI BamHI BbrPI BcgI BciVI
BfrI BglI BglII BlnI BsaAI
BsaBI BseAI BsiEI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BstAPI BstBI BstEII BstPI BstYI
Bsu36I Cfr10I ClaI CpoI DraI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoT22I
FauI FseI HincII HindII HindIII
HpaI KpnI KspI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MseI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TaqI Tru9I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 825 GTATAC GT^[AC][GT]AC
AcsI 849 GAATTC [GA]^AATT[CT]
AcyI 1059 GGCGCC G[GA]^CG[CT]C
AlwNI 323 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 849 GAATTC [GA]^AATT[CT]
AvaI 693 CTCGGG C^[CT]CG[GA]G
AviII 1036 TGCGCA TGC^GCA
BanI 1059 GGCGCC G^G[CT][GA]CC
BbeI 1059 GGCGCC GGCGC^C
BbvCI 922 CCTCAGC CCTCAGC(-5/-2)
BclI 143 TGATCA T^GATCA
BfaI 484 CTAG C^TAG
BlpI 999 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 999 GCTGAGC GC^T[ACGT]AGC
BsaHI 1059 GGCGCC G[GA]^CG[CT]C
BsaI 954 GAGACC GGTCTC(1/5)
BsaWI 1008 TCCGGT [AT]^CCGG[AT]
BsmI 1004 GCATTC GAATGC(1/-1)
BsoBI 693 CTCGGG C^[CT]CG[GA]G
BspMI 718 ACCTGC ACCTGC(4/8)
BsrI 450 ACTGG ACTGG(1/-1)
BssHII 1053 GCGCGC G^CGCGC
BssSI 139 CTCGTG CACGAG(-5/-1)
Bst1107I 825 GTATAC GTA^TAC
BstXI 236 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 825 GTATAC GTA^TAC
CelII 999 GCTGAGC GC^T[ACGT]AGC
DraII 1030 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 427 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1064 CCACGG C^C[GA][CT]GG
EcoNI 291 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1030 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 849 GAATTC G^AATTC
EcoRV 571 GATATC GAT^ATC
FbaI 143 TGATCA T^GATCA
FspI 1036 TGCGCA TGC^GCA
HaeII 1059 GGCGCC [GA]GCGC^[CT]
HgaI 463 GACGC GACGC(5/10)
Hin1I 1059 GGCGCC G[GA]^CG[CT]C
KasI 1059 GGCGCC G^GCGCC
MaeI 484 CTAG C^TAG
NarI 1059 GGCGCC GG^CGCC
NspI 1039 GCATGC [GA]CATG^[CT]
PleI 189 GAGTC GAGTC(4/5)
PshAI 907 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 1059 GGCGCC GGC^GCC
SphI 1039 GCATGC GCATG^C
StuI 707 AGGCCT AGG^CCT
Tsp509I 850 AATT ^AATT
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# TWO CUT ENZYMES ( 15 enzymes) #
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BbsI 301 GTCTTC GAAGAC(2/6)
640 GTCTTC
BpmI 576 CTCCAG CTGGAG(16/14)
244 CTGGAG
BpuAI 301 GTCTTC GAAGAC(2/6)
640 GTCTTC
BseRI 37 CTCCTC GAGGAG(10/8)
550 CTCCTC
BsgI 928 CTGCAC GTGCAG(16/14)
820 GTGCAG
BsmAI 954 GAGAC GTCTC(1/5)
161 GTCTC
Cfr13I 1031 GGGCC G^G[ACGT]CC
1068 GGCCC
EarI 219 CTCTTC CTCTTC(1/4)
951 GAAGAG
Ksp632I 219 CTCTTC CTCTTC(1/4)
951 GAAGAG
MslI 735 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
837 CACTCCCATG
MspA1I 415 CAGCTG C[AC]G^C[GT]G
925 CAGCTG
PstI 320 CTGCAG CTGCA^G
737 CTGCAG
PvuII 415 CAGCTG CAG^CTG
925 CAGCTG
Sau96I 1031 GGGCC G^G[ACGT]CC
1068 GGCCC
TfiI 12 GAATC G^A[AT]TC
154 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AluI BanII BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstUI Cac8I CfoI DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
ScrFI SfaNI SfcI StyI TseI
Tsp45I TspRI