Restriction map for
pFN21AE2053
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI Alw44I
AlwNI ApaLI AscI Asp718I AspI
AvaI AviII AvrII BalI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BsaAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
EclXI Eco52I EcoNI EcoRI EcoT22I
FbaI FseI FspI HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MaeI MamI MluI MluNI MroI
MscI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 1936 AAATTC [GA]^AATT[CT]
AflIII 990 ACGTGT A^C[GA][CT]GT
AhdI 1625 GACCCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1386 AGCGCT AGC^GCT
ApoI 1936 AAATTC [GA]^AATT[CT]
AseI 1393 ATTAAT AT^TAAT
AsnI 1393 ATTAAT AT^TAAT
Asp700I 627 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1625 GACCCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1122 GAGCAC G[AT]GC[AT]^C
BanI 1654 GGCACC G^G[CT][GA]CC
BglI 1646 GCCTACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 43 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 43 GCTGAGC GC^T[ACGT]AGC
BsaWI 617 TCCGGT [AT]^CCGG[AT]
BsiHKAI 1122 GAGCAC G[AT]GC[AT]^C
BsmBI 1632 CGTCTC CGTCTC(1/5)
Bsp1407I 1911 TGTACA T^GTACA
BsrGI 1911 TGTACA T^GTACA
CelII 43 GCTGAGC GC^T[ACGT]AGC
CfoI 1387 GCGC GCG^C
DsaI 1372 CCATGG C^C[GA][CT]GG
Eam1105I 1625 GACCCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1386 AGCGCT AGC^GCT
EcoRV 805 GATATC GAT^ATC
EcoT14I 1372 CCATGG C^C[AT][AT]GG
FauI 691 GCGGG CCCGC(4/6)
HaeII 1386 AGCGCT [GA]GCGC^[CT]
HhaI 1387 GCGC GCG^C
HinP1I 1387 GCGC G^CGC
MaeII 990 ACGT A^CGT
MfeI 281 CAATTG C^AATTG
MslI 1467 CACCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 281 CAATTG C^AATTG
NcoI 1372 CCATGG C^CATGG
NspI 1518 GCATGT [GA]CATG^[CT]
PshBI 1393 ATTAAT AT^TAAT
SapI 349 GAAGAGC GCTCTTC(1/4)
ScaI 1400 AGTACT AGT^ACT
SfcI 1763 CTGTAG C^T[GA][CT]AG
SspBI 1911 TGTACA T^GTACA
StuI 1823 AGGCCT AGG^CCT
StyI 1372 CCATGG C^C[AT][AT]GG
TaqI 1015 TCGA T^CGA
Tsp45I 646 GTGAC ^GT[GC]AC
TthHB8I 1015 TCGA T^CGA
XmnI 627 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 1401 GTAC GT^AC
1912 GTAC
AocI 85 CCTCAGG CC^T[ACGT]AGG
883 CCTAAGG
ApaI 1242 GGGCCC GGGCC^C
1966 GGGCCC
BamHI 1321 GGATCC G^GATCC
1917 GGATCC
BpmI 316 CTCCAG CTGGAG(16/14)
1856 CTGGAG
BsmAI 224 GAGAC GTCTC(1/5)
1633 GTCTC
BsmI 400 GAATGC GAATGC(1/-1)
1079 GCATTC
BsrDI 1587 CATTGC GCAATG(2/0)
1028 GCAATG
BstEII 645 GGTGACC G^GT[ACGT]ACC
1757 GGTTACC
BstPI 645 GGTGACC G^GT[ACGT]ACC
1757 GGTTACC
Bsu36I 85 CCTCAGG CC^T[ACGT]AGG
883 CCTAAGG
Eco81I 85 CCTCAGG CC^T[ACGT]AGG
883 CCTAAGG
EcoO65I 645 GGTGACC G^GT[ACGT]ACC
1757 GGTTACC
HincII 1061 GTCAAC GT[CT]^[GA]AC
2014 GTTGAC
HindII 1061 GTCAAC GT[CT]^[GA]AC
2014 GTTGAC
PvuII 680 CAGCTG CAG^CTG
1946 CAGCTG
RsaI 1401 GTAC GT^AC
1912 GTAC
SmlI 597 CTCAAG C^T[CT][GA]AG
1744 CTCAAG
SspI 482 AATATT AAT^ATT
778 AATATT
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbsI BbvI BcnI BmyI BpuAI
BsaJI BseRI BsgI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp509I TspRI
Van91I XhoII