Restriction map for
pFN21AE6094
##################################
# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiWI BsmBI BsmFI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoRI EcoRV EcoT14I
FauI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
Sau96I ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI StyI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 493 GTCTAC GT^[AC][GT]AC
AciI 414 CCGC CCGC(-3/-1)
AlwNI 762 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 921 CCTGAGG CC^T[ACGT]AGG
AspHI 559 GAGCAC G[AT]GC[AT]^C
AvaI 145 CTCGGG C^[CT]CG[GA]G
BbsI 643 GTCTTC GAAGAC(2/6)
BbvCI 960 GCTGAGG CCTCAGC(-5/-2)
BclI 185 TGATCA T^GATCA
BfaI 617 CTAG C^TAG
BmyI 559 GAGCAC G[AGT]GC[ACT]^C
BpuAI 643 GTCTTC GAAGAC(2/6)
BsiEI 411 CGACCG CG[GA][CT]^CG
BsiHKAI 559 GAGCAC G[AT]GC[AT]^C
BsmI 814 GCATTC GAATGC(1/-1)
BsoBI 145 CTCGGG C^[CT]CG[GA]G
Bsp1286I 559 GAGCAC G[AGT]GC[ACT]^C
BspHI 599 TCATGA T^CATGA
BsrDI 375 CATTGC GCAATG(2/0)
BsrI 529 ACTGG ACTGG(1/-1)
BssSI 675 CACGAG CACGAG(-5/-1)
BstEII 159 GGTCACC G^GT[ACGT]ACC
BstPI 159 GGTCACC G^GT[ACGT]ACC
Bsu36I 921 CCTGAGG CC^T[ACGT]AGG
Eco81I 921 CCTGAGG CC^T[ACGT]AGG
EcoO65I 159 GGTCACC G^GT[ACGT]ACC
EcoT22I 19 ATGCAT ATGCA^T
FbaI 185 TGATCA T^GATCA
MaeI 617 CTAG C^TAG
MspA1I 211 CAGCTG C[AC]G^C[GT]G
NsiI 19 ATGCAT ATGCA^T
PleI 506 GACTC GAGTC(4/5)
PvuII 211 CAGCTG CAG^CTG
RcaI 599 TCATGA T^CATGA
SbfI 26 CCTGCAGG CCTGCA^GG
SmlI 91 CTCAAG C^T[CT][GA]AG
Sse8387I 26 CCTGCAGG CCTGCA^GG
StuI 31 AGGCCT AGG^CCT
Tsp509I 195 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 228 GTAC GT^AC
822 GTAC
BpmI 118 CTCCAG CTGGAG(16/14)
714 CTCCAG
BsgI 214 CTGCAC GTGCAG(16/14)
949 GTGCAG
BsmAI 12 GTCTC GTCTC(1/5)
459 GTCTC
BspMI 469 ACCTGC ACCTGC(4/8)
766 ACCTGC
BssKI 177 CCTGG ^CC[ACGT]GG
855 CCAGG
BstNI 177 CCTGG CC^[AT]GG
855 CCAGG
Cac8I 29 GCAGGC GC[ACGT]^[ACGT]GC
473 GCCTGC
DsaI 788 CCGTGG C^C[GA][CT]GG
884 CCACGG
EarI 139 CTCTTC CTCTTC(1/4)
105 GAAGAG
EcoRII 177 CCTGG ^CC[AT]GG
855 CCAGG
HphI 297 GGTGA GGTGA(8/7)
161 TCACC
Ksp632I 139 CTCTTC CTCTTC(1/4)
105 GAAGAG
MvaI 177 CCTGG CC^[AT]GG
855 CCAGG
NlaIV 173 GGAACC GG[ACGT]^[ACGT]CC
514 GGCTCC
NspI 341 GCATGT [GA]CATG^[CT]
389 ACATGC
RsaI 228 GTAC GT^AC
822 GTAC
ScrFI 177 CCTGG CC^[ACGT]GG
855 CCAGG
TfiI 659 GATTC G^A[AT]TC
903 GAATC
TspRI 74 CAGTG CA[GC]TG[ACGT][ACGT]^
398 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AluI AlwI BbvI BsaJI BseRI
BsiYI BslI BstF5I BstYI DdeI
DpnII Fnu4HI FokI HaeIII HinfI
ItaI MaeIII MboI MboII MflI
MnlI MseI MslI MwoI NdeII
NlaIII PstI Sau3AI SfaNI SfcI
Tru9I TseI Tsp45I XhoII