Restriction map for
pFN21AE5986
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspHI
AspI AvaI AvaII AviII AvrII
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstNI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRI EcoRII EcoRV EcoT22I FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
KspI MaeII MamI MfeI MluI
MroI MunI MvaI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StuI
SwaI TaqI TfiI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 624 GGTACC G^GTACC
AciI 763 GCGG CCGC(-3/-1)
AflIII 338 ACATGT A^C[GA][CT]GT
AlwI 793 GGATC GGATC(4/5)
Asp718I 624 GGTACC G^GTACC
BalI 371 TGGCCA TGG^CCA
BbvI 439 GCTGC GCAGC(8/12)
BclI 134 TGATCA T^GATCA
BcnI 479 CCGGG CC^[GC]GG
BmyI 841 GTGCCC G[AGT]GC[ACT]^C
BsgI 564 CTGCAC GTGCAG(16/14)
BsmAI 744 GTCTC GTCTC(1/5)
BsmFI 408 GTCCC GGGAC(10/14)
Bsp1286I 841 GTGCCC G[AGT]GC[ACT]^C
BspLU11I 338 ACATGT A^CATGT
BsrI 629 CCAGT ACTGG(1/-1)
BssKI 479 CCGGG ^CC[ACGT]GG
BstEII 399 GGTCACC G^GT[ACGT]ACC
BstPI 399 GGTCACC G^GT[ACGT]ACC
BstYI 793 GGATCT [GA]^GATC[CT]
Cfr13I 471 GGCCC G^G[ACGT]CC
DraIII 477 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 371 TGGCCA [CT]^GGCC[GA]
EarI 187 CTCTTC CTCTTC(1/4)
EcoO65I 399 GGTCACC G^GT[ACGT]ACC
FbaI 134 TGATCA T^GATCA
KpnI 624 GGTACC GGTAC^C
Ksp632I 187 CTCTTC CTCTTC(1/4)
MflI 793 GGATCT [GA]^GATC[CT]
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MspA1I 761 CAGCGG C[AC]G^C[GT]G
MwoI 122 GCAACCTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 479 CCGGG CC^[GC]GG
NcoI 113 CCATGG C^CATGG
NdeI 356 CATATG CA^TATG
PstI 504 CTGCAG CTGCA^G
Sau96I 471 GGCCC G^G[ACGT]CC
ScaI 715 AGTACT AGT^ACT
ScrFI 479 CCGGG CC^[ACGT]GG
SmlI 692 CTCAAG C^T[CT][GA]AG
SphI 485 GCATGC GCATG^C
TseI 439 GCTGC G^C[AT]GC
XhoII 793 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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Asp700I 31 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
809 GAAAAGTTTC
BanI 624 GGTACC G^G[CT][GA]CC
840 GGTGCC
BpmI 553 CTCCAG CTGGAG(16/14)
461 CTGGAG
BsiYI 278 CCAATGTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
473 CCCACACCGGG
BslI 278 CCAATGTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
473 CCCACACCGGG
BsmI 671 GCATTC GAATGC(1/-1)
766 GCATTC
BsrDI 386 CATTGC GCAATG(2/0)
735 CATTGC
Cac8I 3 GCTGGC GC[ACGT]^[ACGT]GC
485 GCATGC
DsaI 113 CCATGG C^C[GA][CT]GG
467 CCACGG
EcoT14I 113 CCATGG C^C[AT][AT]GG
126 CCTTGG
Fnu4HI 439 GCTGC GC^[ACGT]GC
763 GCGGC
HaeIII 372 GGCC GG^CC
471 GGCC
HapII 69 CCGG C^CGG
479 CCGG
HincII 342 GTTGAC GT[CT]^[GA]AC
580 GTCAAC
HindII 342 GTTGAC GT[CT]^[GA]AC
580 GTCAAC
HinfI 294 GACTC G^A[ACGT]TC
494 GACTC
HpaII 69 CCGG C^CGG
479 CCGG
ItaI 439 GCTGC GC^[ACGT]GC
763 GCGGC
MspI 69 CCGG C^CGG
479 CCGG
NspI 338 ACATGT [GA]CATG^[CT]
485 GCATGC
PleI 294 GACTC GAGTC(4/5)
494 GACTC
SfcI 504 CTGCAG C^T[GA][CT]AG
864 CTACAG
SspI 268 AATATT AAT^ATT
782 AATATT
StyI 113 CCATGG C^C[AT][AT]GG
126 CCTTGG
TspRI 507 CAGTG CA[GC]TG[ACGT][ACGT]^
896 CACTG
XmnI 31 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
809 GAAAAGTTTC
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AcsI AfaI AluI ApoI BfaI
BsaJI BseRI BstF5I DdeI DpnII
FokI HphI MaeI MaeIII MboI
MboII MnlI MseI MslI NdeII
NlaIII NlaIV RsaI Sau3AI Tru9I
Tsp45I Tsp509I