Restriction map for
pFN21AE5931
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BbvI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
BstYI CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FbaI Fnu4HI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII ItaI KasI KpnI KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TaqI TseI TthHB8I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 442 GTATAC GT^[AC][GT]AC
AluI 724 AGCT AG^CT
AocI 873 CCTGAGG CC^T[ACGT]AGG
AspI 555 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 639 GGTCC G^G[AT]CC
BbsI 819 GTCTTC GAAGAC(2/6)
BciVI 671 GTATCC GTATCC(6/5)
BpuAI 819 GTCTTC GAAGAC(2/6)
BsaI 509 GGTCTC GGTCTC(1/5)
BsmAI 510 GTCTC GTCTC(1/5)
BsmFI 408 GTCCC GGGAC(10/14)
BsmI 286 GAATGC GAATGC(1/-1)
Bst1107I 442 GTATAC GTA^TAC
BstXI 425 CCATAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 442 GTATAC GTA^TAC
Bsu36I 873 CCTGAGG CC^T[ACGT]AGG
Cac8I 43 GCAGGC GC[ACGT]^[ACGT]GC
DsaI 350 CCATGG C^C[GA][CT]GG
EarI 79 CTCTTC CTCTTC(1/4)
Eco81I 873 CCTGAGG CC^T[ACGT]AGG
EcoT22I 288 ATGCAT ATGCA^T
FauI 482 CCCGC CCCGC(4/6)
HaeIII 465 GGCC GG^CC
HphI 246 GGTGA GGTGA(8/7)
Ksp632I 79 CTCTTC CTCTTC(1/4)
MaeIII 109 GTCAC ^GT[ACGT]AC
MwoI 716 GCACTTGTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 350 CCATGG C^CATGG
NlaIV 122 GGAACC GG[ACGT]^[ACGT]CC
NsiI 288 ATGCAT ATGCA^T
NspI 485 GCATGT [GA]CATG^[CT]
PflFI 555 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 729 CCATCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 555 GACTC GAGTC(4/5)
SspI 397 AATATT AAT^ATT
Tsp45I 109 GTCAC ^GT[GC]AC
Tth111I 555 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 729 CCATCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 791 CCAGCACTACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AciI 483 CCGC CCGC(-3/-1)
637 GCGG
AlwNI 23 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
414 CAGGGTCTG
BfaI 668 CTAG C^TAG
914 CTAG
BpmI 170 CTCCAG CTGGAG(16/14)
567 CTCCAG
BsiYI 689 CCACTGAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
729 CCATCTCTTGG
BslI 689 CCACTGAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
729 CCATCTCTTGG
BssKI 39 CCTGG ^CC[ACGT]GG
126 CCTGG
BstF5I 929 CATCC GGATG(2/0)
885 GGATG
BstNI 39 CCTGG CC^[AT]GG
126 CCTGG
Cfr13I 464 GGGCC G^G[ACGT]CC
639 GGTCC
DpnII 135 GATC ^GATC
208 GATC
EcoRII 39 CCTGG ^CC[AT]GG
126 CCTGG
FokI 929 CATCC GGATG(9/13)
885 GGATG
MaeI 668 CTAG C^TAG
914 CTAG
MboI 135 GATC ^GATC
208 GATC
MseI 142 TTAA T^TAA
614 TTAA
MvaI 39 CCTGG CC^[AT]GG
126 CCTGG
NdeII 135 GATC ^GATC
208 GATC
Sau3AI 135 GATC ^GATC
208 GATC
Sau96I 464 GGGCC G^G[ACGT]CC
639 GGTCC
ScrFI 39 CCTGG CC^[ACGT]GG
126 CCTGG
SfaNI 240 GATGC GCATC(5/9)
746 GCATC
SfcI 504 CTGTAG C^T[GA][CT]AG
867 CTATAG
TfiI 588 GATTC G^A[AT]TC
855 GAATC
Tru9I 142 TTAA T^TAA
614 TTAA
Tsp509I 144 AATT ^AATT
324 AATT
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# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
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BsaJI BseRI BsrI DdeI EcoT14I
HinfI MboII MnlI MslI NlaIII
StyI TspRI