Restriction map for
pFN21AE5932
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# NO CUT ENZYMES ( 157 enzymes) #
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Acc65I AccI AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BanII BbeI BbrPI BbsI BcgI
BciVI BcnI BfrI BglII BlnI
BpuAI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BsrDI BsrFI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I Cac8I CfoI Cfr10I ClaI
CpoI DraI DraII DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HapII HhaI HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MfeI MluI MroI
MseI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI Tru9I Tsp509I
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 793 GACGTC GACGT^C
AflIII 203 ACATGT A^C[GA][CT]GT
AvaI 618 CTCGGG C^[CT]CG[GA]G
BamHI 677 GGATCC G^GATCC
BanI 225 GGTGCC G^G[CT][GA]CC
BbvCI 572 CCTCAGC CCTCAGC(-5/-2)
BclI 137 TGATCA T^GATCA
BfaI 610 CTAG C^TAG
BglI 421 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 292 GCTCAGC GC^T[ACGT]AGC
BpmI 786 CTCCAG CTGGAG(16/14)
Bpu1102I 292 GCTCAGC GC^T[ACGT]AGC
BsaAI 97 TACGTG [CT]AC^GT[GA]
BsaBI 585 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 777 GAGACC GGTCTC(1/5)
BseRI 121 CTCCTC GAGGAG(10/8)
BsoBI 618 CTCGGG C^[CT]CG[GA]G
Bsp1407I 857 TGTACA T^GTACA
BspLU11I 203 ACATGT A^CATGT
BspMI 413 GCAGGT ACCTGC(4/8)
BsrBI 382 CCGCTC CCGCTC(-3/-3)
BsrGI 857 TGTACA T^GTACA
BsrI 555 ACTGG ACTGG(1/-1)
BssSI 16 CTCGTG CACGAG(-5/-1)
BstXI 578 CCAACGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 292 GCTCAGC GC^T[ACGT]AGC
DraIII 324 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 243 GACCTTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 245 CCTTGG C^C[AT][AT]GG
FauI 381 CCCGC CCCGC(4/6)
FbaI 137 TGATCA T^GATCA
HgaI 64 GACGC GACGC(5/10)
MaeI 610 CTAG C^TAG
MamI 585 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 282 CAGCTG C[AC]G^C[GT]G
NspI 203 ACATGT [GA]CATG^[CT]
PvuII 282 CAGCTG CAG^CTG
SspBI 857 TGTACA T^GTACA
StyI 245 CCTTGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 64 GACGCC G[GA]^CG[CT]C
793 GACGTC
AspHI 100 GTGCTC G[AT]GC[AT]^C
321 GAGCAC
AvaII 505 GGACC G^G[AT]CC
738 GGTCC
BbvI 284 GCTGC GCAGC(8/12)
623 GCTGC
BsaHI 64 GACGCC G[GA]^CG[CT]C
793 GACGTC
BsgI 457 CTGCAC GTGCAG(16/14)
563 GTGCAG
BsiHKAI 100 GTGCTC G[AT]GC[AT]^C
321 GAGCAC
BsmAI 777 GAGAC GTCTC(1/5)
905 GAGAC
BsmFI 791 GGGAC GGGAC(10/14)
252 GTCCC
BstYI 515 AGATCC [GA]^GATC[CT]
677 GGATCC
Hin1I 64 GACGCC G[GA]^CG[CT]C
793 GACGTC
HinfI 80 GAATC G^A[ACGT]TC
148 GATTC
MaeII 98 ACGT A^CGT
794 ACGT
MboII 882 GAAGA GAAGA(8/7)
83 TCTTC
MflI 515 AGATCC [GA]^GATC[CT]
677 GGATCC
SfaNI 535 GATGC GCATC(5/9)
731 GCATC
TfiI 80 GAATC G^A[AT]TC
148 GATTC
TseI 284 GCTGC G^C[AT]GC
623 GCTGC
Tsp45I 19 GTGAC ^GT[GC]AC
220 GTCAC
XcmI 545 CCATCCTGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
788 CCAGGGACGTCGTGG
XhoII 515 AGATCC [GA]^GATC[CT]
677 GGATCC
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AfaI AluI AlwI BalI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HphI ItaI MaeIII MboI
MluNI MnlI MscI MslI MvaI
MwoI NdeII NlaIII NlaIV PflMI
RsaI Sau3AI Sau96I ScrFI TspRI
Van91I