Restriction map for
pFN21AE6046
##################################
# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII BamHI
BanI BbeI BbrPI BbsI BcgI
BciVI BcnI BfrI BglI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HindIII
HpaI HpaII KasI KpnI KspI
MamI MluI MroI MspI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 711 GTCTAC GT^[AC][GT]AC
Alw44I 422 GTGCAC G^TGCAC
AlwI 646 GGATC GGATC(4/5)
AlwNI 382 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 422 GTGCAC G^TGCAC
AvrII 235 CCTAGG C^CTAGG
BalI 887 TGGCCA TGG^CCA
BanII 46 GGGCTC G[GA]GC[CT]^C
BbvCI 544 CCTCAGC CCTCAGC(-5/-2)
BglII 332 AGATCT A^GATCT
BlnI 235 CCTAGG C^CTAGG
BpmI 934 CTGGAG CTGGAG(16/14)
BseRI 906 GAGGAG GAGGAG(10/8)
BsmAI 811 GTCTC GTCTC(1/5)
BsmBI 810 CGTCTC CGTCTC(1/5)
BsmFI 929 GTCCC GGGAC(10/14)
BspMI 161 ACCTGC ACCTGC(4/8)
BsrI 42 ACTGG ACTGG(1/-1)
BstXI 312 CCACTTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 332 AGATCT [GA]^GATC[CT]
Cac8I 562 GCCTGC GC[ACGT]^[ACGT]GC
DrdI 802 GACAAGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 797 CCATGG C^C[GA][CT]GG
EaeI 887 TGGCCA [CT]^GGCC[GA]
HincII 247 GTTGAC GT[CT]^[GA]AC
HindII 247 GTTGAC GT[CT]^[GA]AC
MaeII 767 ACGT A^CGT
MfeI 827 CAATTG C^AATTG
MflI 332 AGATCT [GA]^GATC[CT]
MluNI 887 TGGCCA TGG^CCA
MscI 887 TGGCCA TGG^CCA
MunI 827 CAATTG C^AATTG
NcoI 797 CCATGG C^CATGG
PflMI 499 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 294 GACTC GAGTC(4/5)
PpuMI 917 AGGACCC [GA]G^G[AT]CC[CT]
SfcI 113 CTGTAG C^T[GA][CT]AG
StuI 778 AGGCCT AGG^CCT
Van91I 499 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 332 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 363 CCGC CCGC(-3/-1)
696 CCGC
AfaI 472 GTAC GT^AC
651 GTAC
AspHI 422 GTGCAC G[AT]GC[AT]^C
937 GAGCAC
AvaII 918 GGACC G^G[AT]CC
928 GGTCC
BclI 551 TGATCA T^GATCA
881 TGATCA
BfaI 236 CTAG C^TAG
664 CTAG
BsiHKAI 422 GTGCAC G[AT]GC[AT]^C
937 GAGCAC
BsrDI 369 CATTGC GCAATG(2/0)
729 CATTGC
BstEII 615 GGTGACC G^GT[ACGT]ACC
738 GGTGACC
BstPI 615 GGTGACC G^GT[ACGT]ACC
738 GGTGACC
DraII 704 AGGCCCT [GA]G^G[ACGT]CC[CT]
917 AGGACCC
EarI 219 CTCTTC CTCTTC(1/4)
301 CTCTTC
EcoO109I 704 AGGCCCT [GA]G^G[ACGT]CC[CT]
917 AGGACCC
EcoO65I 615 GGTGACC G^GT[ACGT]ACC
738 GGTGACC
EcoT14I 235 CCTAGG C^C[AT][AT]GG
797 CCATGG
FbaI 551 TGATCA T^GATCA
881 TGATCA
Ksp632I 219 CTCTTC CTCTTC(1/4)
301 CTCTTC
MaeI 236 CTAG C^TAG
664 CTAG
MseI 592 TTAA T^TAA
745 TTAA
MspA1I 493 CAGCTG C[AC]G^C[GT]G
547 CAGCTG
NlaIV 918 GGACCC GG[ACGT]^[ACGT]CC
928 GGTCCC
PvuII 493 CAGCTG CAG^CTG
547 CAGCTG
RsaI 472 GTAC GT^AC
651 GTAC
StyI 235 CCTAGG C^C[AT][AT]GG
797 CCATGG
Tru9I 592 TTAA T^TAA
745 TTAA
Tsp509I 557 AATT ^AATT
828 AATT
TspRI 84 CACTG CA[GC]TG[ACGT][ACGT]^
659 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AluI BbvI BmyI BsaJI BsgI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeIII MboI MboII MnlI
MslI MvaI MwoI NdeII NlaIII
Sau3AI Sau96I ScrFI SfaNI TfiI
TseI Tsp45I