Restriction map for
pFN21AE2026
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# NO CUT ENZYMES ( 105 enzymes) #
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Acc65I AccI AclI AflII AflIII
AgeI AhdI AocI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AvrII BamHI BbeI BbsI
BciVI BclI BfrI BglII BlnI
BpuAI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI KpnI
MfeI MluI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SalI
SapI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI SspBI
SspI SwaI TfiI Tru9I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 1315 GACGTC GACGT^C
AccIII 1304 TCCGGA T^CCGGA
AcsI 1348 GAATTC [GA]^AATT[CT]
Alw44I 916 GTGCAC G^TGCAC
AlwNI 237 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 916 GTGCAC G^TGCAC
ApoI 1348 GAATTC [GA]^AATT[CT]
AspI 364 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 107 TGCGCA TGC^GCA
BanI 7 GGCACC G^G[CT][GA]CC
BbrPI 685 CACGTG CAC^GTG
BglI 954 GCCTGACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 685 CACGTG [CT]AC^GT[GA]
BsaBI 1105 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1304 TCCGGA [AT]^CCGG[AT]
BseAI 1304 TCCGGA T^CCGGA
BsmBI 606 CGTCTC CGTCTC(1/5)
BspEI 1304 TCCGGA T^CCGGA
BsrBI 580 GAGCGG CCGCTC(-3/-3)
BsrFI 866 ACCGGC [GA]^CCGG[CT]
BstAPI 99 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1038 GGTGACC G^GT[ACGT]ACC
BstPI 1038 GGTGACC G^GT[ACGT]ACC
BstXI 464 CCATGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 866 ACCGGC [GA]^CCGG[CT]
DrdI 565 GACAACCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 595 CTCTTC CTCTTC(1/4)
EcoO65I 1038 GGTGACC G^GT[ACGT]ACC
EcoRI 1348 GAATTC G^AATTC
FspI 107 TGCGCA TGC^GCA
HaeII 1384 AGCGCC [GA]GCGC^[CT]
Ksp632I 595 CTCTTC CTCTTC(1/4)
KspI 51 CCGCGG CCGC^GG
MamI 1105 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1304 TCCGGA T^CCGGA
MslI 723 CACGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 110 GCATGT [GA]CATG^[CT]
PflFI 364 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 685 CACGTG CAC^GTG
PmlI 685 CACGTG CAC^GTG
SacII 51 CCGCGG CCGC^GG
SbfI 210 CCTGCAGG CCTGCA^GG
SpeI 834 ACTAGT A^CTAGT
Sse8387I 210 CCTGCAGG CCTGCA^GG
StuI 1762 AGGCCT AGG^CCT
Tth111I 364 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 869 GGCGTC G[GA]^CG[CT]C
1315 GACGTC
ApaI 659 GGGCCC GGGCC^C
1652 GGGCCC
AspHI 916 GTGCAC G[AT]GC[AT]^C
1273 GAGCAC
BalI 1031 TGGCCA TGG^CCA
1329 TGGCCA
BbvCI 633 CCTCAGC CCTCAGC(-5/-2)
1380 CCTCAGC
BcgI 347 GCAGCCTAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1481 GCATGGGCTTCG
BfaI 319 CTAG C^TAG
835 CTAG
BlpI 289 GCTGAGC GC^T[ACGT]AGC
1080 GCTGAGC
Bpu1102I 289 GCTGAGC GC^T[ACGT]AGC
1080 GCTGAGC
BsaHI 869 GGCGTC G[GA]^CG[CT]C
1315 GACGTC
BsiHKAI 916 GTGCAC G[AT]GC[AT]^C
1273 GAGCAC
BspHI 119 TCATGA T^CATGA
626 TCATGA
BssSI 1185 CACGAG CACGAG(-5/-1)
1264 CTCGTG
CelII 289 GCTGAGC GC^T[ACGT]AGC
1080 GCTGAGC
EcoNI 18 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
519 CCTGTTTCAGG
EcoT14I 464 CCATGG C^C[AT][AT]GG
1034 CCATGG
Hin1I 869 GGCGTC G[GA]^CG[CT]C
1315 GACGTC
MaeI 319 CTAG C^TAG
835 CTAG
MluNI 1031 TGGCCA TGG^CCA
1329 TGGCCA
MscI 1031 TGGCCA TGG^CCA
1329 TGGCCA
NcoI 464 CCATGG C^CATGG
1034 CCATGG
RcaI 119 TCATGA T^CATGA
626 TCATGA
SexAI 230 ACCTGGT A^CC[AT]GGT
929 ACCTGGT
SmlI 772 CTCAAG C^T[CT][GA]AG
922 CTCAAG
StyI 464 CCATGG C^C[AT][AT]GG
1034 CCATGG
Tsp509I 205 AATT ^AATT
1349 AATT
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BanII BbvI BcnI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TseI Tsp45I TspRI TthHB8I XcmI
XhoII