Restriction map for
pFN21AE6006
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvI
BcgI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstF5I BstUI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
Fnu4HI FokI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI ItaI KasI KpnI KspI
MamI MfeI MluI MroI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StyI SwaI TaqI TseI
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AcsI 31 GAATTT [GA]^AATT[CT]
AlwI 3 GGATC GGATC(4/5)
AocI 235 CCTAAGG CC^T[ACGT]AGG
ApoI 31 GAATTT [GA]^AATT[CT]
AspHI 586 GAGCTC G[AT]GC[AT]^C
AvaI 59 CCCGAG C^[CT]CG[GA]G
BalI 887 TGGCCA TGG^CCA
BanII 586 GAGCTC G[GA]GC[CT]^C
BcnI 776 CCCGG CC^[GC]GG
BfaI 913 CTAG C^TAG
BglII 249 AGATCT A^GATCT
BpmI 583 CTGGAG CTGGAG(16/14)
BsaI 677 GGTCTC GGTCTC(1/5)
BseRI 640 CTCCTC GAGGAG(10/8)
BsiHKAI 586 GAGCTC G[AT]GC[AT]^C
BsmBI 906 GAGACG CGTCTC(1/5)
BsoBI 59 CCCGAG C^[CT]CG[GA]G
Bsp1407I 101 TGTACA T^GTACA
BspHI 404 TCATGA T^CATGA
BspMI 715 ACCTGC ACCTGC(4/8)
BsrGI 101 TGTACA T^GTACA
BsrI 685 ACTGG ACTGG(1/-1)
BstAPI 747 GCATTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 738 GGTGACC G^GT[ACGT]ACC
BstPI 738 GGTGACC G^GT[ACGT]ACC
BstYI 249 AGATCT [GA]^GATC[CT]
Bsu36I 235 CCTAAGG CC^T[ACGT]AGG
Cac8I 562 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 472 GCGC GCG^C
DsaI 734 CCGTGG C^C[GA][CT]GG
EaeI 887 TGGCCA [CT]^GGCC[GA]
EarI 903 GAAGAG CTCTTC(1/4)
Eco81I 235 CCTAAGG CC^T[ACGT]AGG
EcoO65I 738 GGTGACC G^GT[ACGT]ACC
HapII 777 CCGG C^CGG
HhaI 472 GCGC GCG^C
HinP1I 472 GCGC G^CGC
HpaII 777 CCGG C^CGG
Ksp632I 903 GAAGAG CTCTTC(1/4)
MaeI 913 CTAG C^TAG
MaeII 332 ACGT A^CGT
MflI 249 AGATCT [GA]^GATC[CT]
MluNI 887 TGGCCA TGG^CCA
MscI 887 TGGCCA TGG^CCA
MseI 934 TTAA T^TAA
MspI 777 CCGG C^CGG
MwoI 747 GCATTTCGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 776 CCCGG CC^[GC]GG
NlaIV 508 GGACCC GG[ACGT]^[ACGT]CC
PflMI 312 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 404 TCATGA T^CATGA
SacI 586 GAGCTC GAGCT^C
SspBI 101 TGTACA T^GTACA
StuI 426 AGGCCT AGG^CCT
Tru9I 934 TTAA T^TAA
Van91I 312 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 249 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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AciI 189 CCGC CCGC(-3/-1)
363 CCGC
AfaI 102 GTAC GT^AC
177 GTAC
AluI 231 AGCT AG^CT
587 AGCT
AlwNI 282 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
800 CAGACACTG
BbvCI 672 GCTGAGG CCTCAGC(-5/-2)
864 GCTGAGG
BmyI 586 GAGCTC G[AGT]GC[ACT]^C
754 GTGCCC
BsmFI 57 GTCCC GGGAC(10/14)
541 GTCCC
Bsp1286I 586 GAGCTC G[AGT]GC[ACT]^C
754 GTGCCC
DpnII 4 GATC ^GATC
250 GATC
DraII 239 AGGTCCT [GA]G^G[ACGT]CC[CT]
548 AGGTCCT
EcoO109I 239 AGGTCCT [GA]G^G[ACGT]CC[CT]
548 AGGTCCT
HinfI 154 GAATC G^A[ACGT]TC
870 GAATC
MboI 4 GATC ^GATC
250 GATC
NdeII 4 GATC ^GATC
250 GATC
PpuMI 239 AGGTCCT [GA]G^G[AT]CC[CT]
548 AGGTCCT
RsaI 102 GTAC GT^AC
177 GTAC
Sau3AI 4 GATC ^GATC
250 GATC
TfiI 154 GAATC G^A[AT]TC
870 GAATC
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
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AvaII BsaJI BsgI BsiYI BslI
BsmAI BssKI BstNI Cfr13I DdeI
EcoRII HaeIII HphI MaeIII MboII
MnlI MslI MvaI NlaIII Sau96I
ScrFI SfaNI Tsp45I Tsp509I TspRI