Restriction map for
pFN21AE5926
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BcgI BciVI BclI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrI BssHII
Bst1107I BstBI BstUI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MseI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TaqI TfiI Tru9I Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AciI 189 CCGC CCGC(-3/-1)
AcsI 31 GAATTT [GA]^AATT[CT]
AhdI 250 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 286 AGCT AG^CT
ApoI 31 GAATTT [GA]^AATT[CT]
AspEI 250 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 371 TGGCCA TGG^CCA
BbvCI 672 GCTGAGG CCTCAGC(-5/-2)
BcnI 776 CCCGG CC^[GC]GG
BfaI 196 CTAG C^TAG
BglII 476 AGATCT A^GATCT
BmyI 754 GTGCCC G[AGT]GC[ACT]^C
BsaI 677 GGTCTC GGTCTC(1/5)
Bsp1286I 754 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 101 TGTACA T^GTACA
BspHI 404 TCATGA T^CATGA
BsrDI 417 GCAATG GCAATG(2/0)
BsrGI 101 TGTACA T^GTACA
BssSI 913 CTCGTG CACGAG(-5/-1)
BstAPI 747 GCATTTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 312 CCACCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 476 AGATCT [GA]^GATC[CT]
DraII 548 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 391 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 371 TGGCCA [CT]^GGCC[GA]
Eam1105I 250 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 548 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 318 CCTTGG C^C[AT][AT]GG
HapII 777 CCGG C^CGG
HgaI 669 GACGC GACGC(5/10)
HpaII 777 CCGG C^CGG
MaeI 196 CTAG C^TAG
MflI 476 AGATCT [GA]^GATC[CT]
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MspI 777 CCGG C^CGG
MwoI 747 GCATTTTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 776 CCCGG CC^[GC]GG
PflMI 66 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 548 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 404 TCATGA T^CATGA
SfaNI 761 GCATC GCATC(5/9)
SmlI 583 CTTGAG C^T[CT][GA]AG
SspBI 101 TGTACA T^GTACA
StyI 318 CCTTGG C^C[AT][AT]GG
Tsp509I 32 AATT ^AATT
Van91I 66 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 476 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 102 GTAC GT^AC
177 GTAC
BbsI 896 GAAGAC GAAGAC(2/6)
908 GAAGAC
BbvI 287 GCTGC GCAGC(8/12)
489 GCTGC
BpuAI 896 GAAGAC GAAGAC(2/6)
908 GAAGAC
BsaJI 318 CCTTGG C^C[ACGT][ACGT]GG
545 CCCAGG
BseRI 127 CTCCTC GAGGAG(10/8)
219 CTCCTC
BsgI 388 CTGCAC GTGCAG(16/14)
472 GTGCAG
BsmAI 156 GTCTC GTCTC(1/5)
678 GTCTC
BsmFI 45 GGGAC GGGAC(10/14)
541 GTCCC
BstEII 138 GGTCACC G^GT[ACGT]ACC
738 GGTGACC
BstPI 138 GGTCACC G^GT[ACGT]ACC
738 GGTGACC
DpnII 477 GATC ^GATC
855 GATC
DrdI 147 GACCTGTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
669 GACGCTGAGGTC
EarI 307 CTCTTC CTCTTC(1/4)
903 GAAGAG
EcoO65I 138 GGTCACC G^GT[ACGT]ACC
738 GGTGACC
Fnu4HI 287 GCTGC GC^[ACGT]GC
489 GCTGC
ItaI 287 GCTGC GC^[ACGT]GC
489 GCTGC
Ksp632I 307 CTCTTC CTCTTC(1/4)
903 GAAGAG
MboI 477 GATC ^GATC
855 GATC
NdeII 477 GATC ^GATC
855 GATC
NlaIV 508 GGACCC GG[ACGT]^[ACGT]CC
869 GGAACC
RsaI 102 GTAC GT^AC
177 GTAC
Sau3AI 477 GATC ^GATC
855 GATC
TseI 287 GCTGC G^C[AT]GC
489 GCTGC
XcmI 312 CCACCTCCTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
602 CCAACAATGGCCTGG
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AvaII BsiYI BslI BssKI BstF5I
BstNI Cfr13I DdeI EcoRII FokI
HaeIII HinfI HphI MaeIII MboII
MnlI MslI MvaI NlaIII PleI
Sau96I ScrFI Tsp45I TspRI