Restriction map for
pFN21AE5991
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI AocI Aor51HI ApaI ApoI
AscI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
MvnI NarI NciI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tth111I TthHB8I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AfaI 177 GTAC GT^AC
AflIII 52 ACACGT A^C[GA][CT]GT
Alw44I 717 GTGCAC G^TGCAC
AlwNI 895 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 717 GTGCAC G^TGCAC
AseI 817 ATTAAT AT^TAAT
AsnI 817 ATTAAT AT^TAAT
AvaI 59 CCCGAG C^[CT]CG[GA]G
AvaII 3 GGACC G^G[AT]CC
BanII 645 GGGCTC G[GA]GC[CT]^C
BbrPI 714 CACGTG CAC^GTG
BbsI 88 GTCTTC GAAGAC(2/6)
BglII 299 AGATCT A^GATCT
BpuAI 88 GTCTTC GAAGAC(2/6)
BsaAI 714 CACGTG [CT]AC^GT[GA]
BseRI 906 GAGGAG GAGGAG(10/8)
BsmAI 909 GAGAC GTCTC(1/5)
BsmI 462 GCATTC GAATGC(1/-1)
BsoBI 59 CCCGAG C^[CT]CG[GA]G
BsrFI 776 GCCGGC [GA]^CCGG[CT]
BstAPI 894 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 615 GGTGACC G^GT[ACGT]ACC
BstPI 615 GGTGACC G^GT[ACGT]ACC
Cfr10I 776 GCCGGC [GA]^CCGG[CT]
DraII 704 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 797 CCATGG C^C[GA][CT]GG
EarI 743 CTCTTC CTCTTC(1/4)
EcoO109I 704 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 615 GGTGACC G^GT[ACGT]ACC
FauI 158 CCCGC CCCGC(4/6)
HapII 777 CCGG C^CGG
HgaI 789 GACGC GACGC(5/10)
HpaII 777 CCGG C^CGG
Ksp632I 743 CTCTTC CTCTTC(1/4)
MseI 818 TTAA T^TAA
MspI 777 CCGG C^CGG
NaeI 776 GCCGGC GCC^GGC
NcoI 797 CCATGG C^CATGG
NgoMIV 776 GCCGGC G^CCGGC
NspI 728 ACATGC [GA]CATG^[CT]
PflMI 726 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 714 CACGTG CAC^GTG
PmlI 714 CACGTG CAC^GTG
PshBI 817 ATTAAT AT^TAAT
PstI 901 CTGCAG CTGCA^G
RsaI 177 GTAC GT^AC
SfcI 901 CTGCAG C^T[GA][CT]AG
Tru9I 818 TTAA T^TAA
Tsp509I 480 AATT ^AATT
Van91I 726 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AluI 65 AGCT AG^CT
587 AGCT
AlwI 677 GATCC GGATC(4/5)
249 GGATC
AspHI 541 GTGCTC G[AT]GC[AT]^C
717 GTGCAC
BpmI 544 CTCCAG CTGGAG(16/14)
67 CTGGAG
BsgI 388 CTGCAC GTGCAG(16/14)
564 CTGCAC
BsiHKAI 541 GTGCTC G[AT]GC[AT]^C
717 GTGCAC
BspHI 593 TCATGA T^CATGA
785 TCATGA
BstF5I 471 CATCC GGATG(2/0)
522 GGATG
Cac8I 562 GCCTGC GC[ACGT]^[ACGT]GC
776 GCCGGC
DdeI 673 CTGAG C^T[ACGT]AG
865 CTGAG
DraIII 391 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
748 CACTTCGTG
EcoT14I 428 CCTTGG C^C[AT][AT]GG
797 CCATGG
FokI 471 CATCC GGATG(9/13)
522 GGATG
HinfI 154 GAGTC G^A[ACGT]TC
741 GACTC
MwoI 319 GCTGGTGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
894 GCAGAGGCTGC
PleI 741 GACTC GAGTC(4/5)
154 GAGTC
RcaI 593 TCATGA T^CATGA
785 TCATGA
StyI 428 CCTTGG C^C[AT][AT]GG
797 CCATGG
XcmI 236 CCAAGTTCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
312 CCACTTTGCTGGTGG
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI BbvI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstNI BstXI BstYI Cfr13I DpnII
EcoRII Fnu4HI HaeIII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MslI MvaI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TseI Tsp45I TspRI XhoII