Restriction map for
pFN21AE6016
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbvCI BciVI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BsiWI
BsmAI BsmBI BsmI BsoBI BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT14I EcoT22I FauI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MseI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI TaqI Tru9I Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 603 GAATTT [GA]^AATT[CT]
ApoI 603 GAATTT [GA]^AATT[CT]
AspHI 471 GAGCTC G[AT]GC[AT]^C
BanII 471 GAGCTC G[GA]GC[CT]^C
BbrPI 726 CACGTG CAC^GTG
BbsI 276 GAAGAC GAAGAC(2/6)
BcgI 369 CGACCGATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 152 TGATCA T^GATCA
BfaI 811 CTAG C^TAG
BmyI 471 GAGCTC G[AGT]GC[ACT]^C
BpuAI 276 GAAGAC GAAGAC(2/6)
BsaAI 726 CACGTG [CT]AC^GT[GA]
BsgI 172 CTGCAC GTGCAG(16/14)
BsiEI 369 CGACCG CG[GA][CT]^CG
BsiHKAI 471 GAGCTC G[AT]GC[AT]^C
Bsp1286I 471 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 430 TGTACA T^GTACA
BspHI 413 TCATGA T^CATGA
BsrGI 430 TGTACA T^GTACA
BsrI 556 CCAGT ACTGG(1/-1)
BssSI 610 CTCGTG CACGAG(-5/-1)
BstEII 618 GGTCACC G^GT[ACGT]ACC
BstPI 618 GGTCACC G^GT[ACGT]ACC
BstYI 689 AGATCC [GA]^GATC[CT]
EarI 228 CTCTTC CTCTTC(1/4)
EcoO65I 618 GGTCACC G^GT[ACGT]ACC
FbaI 152 TGATCA T^GATCA
Ksp632I 228 CTCTTC CTCTTC(1/4)
MaeI 811 CTAG C^TAG
MaeII 727 ACGT A^CGT
MflI 689 AGATCC [GA]^GATC[CT]
MwoI 464 GCTTCCTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 452 CATATG CA^TATG
PflMI 11 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 753 GACTC GAGTC(4/5)
PmaCI 726 CACGTG CAC^GTG
PmlI 726 CACGTG CAC^GTG
RcaI 413 TCATGA T^CATGA
SacI 471 GAGCTC GAGCT^C
SspBI 430 TGTACA T^GTACA
TfiI 56 GATTC G^A[AT]TC
Tsp509I 604 AATT ^AATT
Van91I 11 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 281 CCATATCATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 689 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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AciI 515 GCGG CCGC(-3/-1)
679 GCGG
AflIII 725 ACACGT A^C[GA][CT]GT
727 ACGTGT
AvaII 517 GGACC G^G[AT]CC
681 GGTCC
BbvI 379 GCAGC GCAGC(8/12)
301 GCTGC
BseRI 85 CTCCTC GAGGAG(10/8)
346 CTCCTC
BsmFI 766 GGGAC GGGAC(10/14)
818 GGGAC
Cac8I 487 GCCAGC GC[ACGT]^[ACGT]GC
718 GCCTGC
DraII 80 AGGCCCT [GA]G^G[ACGT]CC[CT]
422 AGGGCCT
EcoNI 477 CCTGATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
909 CCTGTGGAAGG
EcoO109I 80 AGGCCCT [GA]G^G[ACGT]CC[CT]
422 AGGGCCT
Fnu4HI 301 GCTGC GC^[ACGT]GC
379 GCAGC
HinfI 56 GATTC G^A[ACGT]TC
753 GACTC
ItaI 301 GCTGC GC^[ACGT]GC
379 GCAGC
MslI 177 CACACCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
408 CACTATCATG
TseI 301 GCTGC G^C[AT]GC
379 GCAGC
TspRI 32 CAGTG CA[GC]TG[ACGT][ACGT]^
255 CACTG
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AfaI AluI AlwI BpmI BsaJI
BsiYI BslI BssKI BstF5I BstNI
Cfr13I DdeI DpnII EcoRII FokI
HaeIII HphI MaeIII MboI MboII
MnlI MvaI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
Tsp45I