Restriction map for
pFN21AE5927
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI KspI
MaeII MfeI MflI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SbfI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AlwI 68 GGATC GGATC(4/5)
AspHI 79 GTGCTC G[AT]GC[AT]^C
AvaII 704 GGTCC G^G[AT]CC
BfaI 637 CTAG C^TAG
BpmI 794 CTGGAG CTGGAG(16/14)
BsaBI 818 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 79 GTGCTC G[AT]GC[AT]^C
BsmAI 341 GTCTC GTCTC(1/5)
BsmI 712 GCATTC GAATGC(1/-1)
Bsp1407I 585 TGTACA T^GTACA
BspMI 701 GCAGGT ACCTGC(4/8)
BsrGI 585 TGTACA T^GTACA
BstXI 501 CCACTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 704 GGTCC G^G[ACGT]CC
DsaI 353 CCATGG C^C[GA][CT]GG
EcoRV 648 GATATC GAT^ATC
HindIII 807 AAGCTT A^AGCTT
MaeI 637 CTAG C^TAG
MamI 818 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 740 CAGCTG C[AC]G^C[GT]G
NcoI 353 CCATGG C^CATGG
NspI 542 ACATGC [GA]CATG^[CT]
PleI 334 GAGTC GAGTC(4/5)
PvuII 740 CAGCTG CAG^CTG
SapI 81 GCTCTTC GCTCTTC(1/4)
Sau96I 704 GGTCC G^G[ACGT]CC
ScaI 302 AGTACT AGT^ACT
SspBI 585 TGTACA T^GTACA
SspI 16 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 20 enzymes) #
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BbvI 373 GCTGC GCAGC(8/12)
929 GCTGC
BmyI 79 GTGCTC G[AGT]GC[ACT]^C
632 GTGCCC
BsiYI 255 CCTCTTACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
902 CCTTAAAGAGG
BslI 255 CCTCTTACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
902 CCTTAAAGAGG
BsmFI 324 GGGAC GGGAC(10/14)
235 GTCCC
Bsp1286I 79 GTGCTC G[AGT]GC[ACT]^C
632 GTGCCC
EarI 82 CTCTTC CTCTTC(1/4)
751 CTCTTC
EcoNI 255 CCTCTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
902 CCTTAAAGAGG
EcoT14I 353 CCATGG C^C[AT][AT]GG
707 CCAAGG
Fnu4HI 373 GCTGC GC^[ACGT]GC
929 GCTGC
HaeIII 120 GGCC GG^CC
457 GGCC
ItaI 373 GCTGC GC^[ACGT]GC
929 GCTGC
Ksp632I 82 CTCTTC CTCTTC(1/4)
751 CTCTTC
MwoI 218 GCTATATCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
659 GCTATATTGGC
NlaIV 125 GGAACC GG[ACGT]^[ACGT]CC
442 GGAGCC
StyI 353 CCATGG C^C[AT][AT]GG
707 CCAAGG
TseI 373 GCTGC G^C[AT]GC
929 GCTGC
Tsp45I 411 GTCAC ^GT[GC]AC
539 GTGAC
Tsp509I 480 AATT ^AATT
891 AATT
TspRI 462 CACTG CA[GC]TG[ACGT][ACGT]^
837 CACTG
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
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AfaI AluI BsaJI BspHI BsrI
BssKI BstF5I BstNI DdeI DpnII
EcoRII FokI HinfI HphI MaeIII
MboI MboII MnlI MseI MslI
MvaI NdeII NlaIII RcaI RsaI
Sau3AI ScrFI SfaNI TfiI Tru9I