Restriction map for
pFN21AE3247
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
Acc65I AclI AfaI AflII AflIII
AhdI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BclI BfrI BlnI BlpI Bpu1102I
BsaAI BsgI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraI DraII DraIII
DrdI Eam1105I Eco47III EcoO109I EcoO65I
EcoRI FbaI FseI FspI HindIII
HpaI KpnI KspI MfeI MluI
MluNI MscI MunI NcoI NdeI
NheI NruI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SacII ScaI SexAI
SfuI SmlI SnaBI SpeI SphI
SspBI SwaI Tsp45I Tth111I XhoI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 268 GACGTC GACGT^C
AccI 1226 GTCGAC GT^[AC][GT]AC
AccIII 75 TCCGGA T^CCGGA
AcsI 1312 AAATTT [GA]^AATT[CT]
AgeI 523 ACCGGT A^CCGGT
Alw44I 1165 GTGCAC G^TGCAC
AlwNI 1694 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1165 GTGCAC G^TGCAC
ApoI 1312 AAATTT [GA]^AATT[CT]
BbvCI 147 GCTGAGG CCTCAGC(-5/-2)
BcgI 334 CGACTCCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 225 GTATCC GTATCC(6/5)
BseAI 75 TCCGGA T^CCGGA
BspEI 75 TCCGGA T^CCGGA
BstAPI 1071 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DsaI 736 CCACGG C^C[GA][CT]GG
EagI 182 CGGCCG C^GGCCG
EclXI 182 CGGCCG C^GGCCG
Eco52I 182 CGGCCG C^GGCCG
EcoNI 408 CCTCCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1420 GATATC GAT^ATC
EcoT22I 342 ATGCAT ATGCA^T
HgaI 1449 GACGC GACGC(5/10)
HincII 1226 GTCGAC GT[CT]^[GA]AC
HindII 1226 GTCGAC GT[CT]^[GA]AC
MaeIII 355 GTAAC ^GT[ACGT]AC
MroI 75 TCCGGA T^CCGGA
MslI 1196 CATTACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 233 GCCGGC GCC^GGC
NgoMIV 233 GCCGGC G^CCGGC
NotI 181 GCGGCCGC GC^GGCCGC
NsiI 342 ATGCAT ATGCA^T
NspI 1083 ACATGC [GA]CATG^[CT]
PflMI 1152 CCACAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 523 ACCGGT A^CCGGT
PshAI 880 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 197 CAGCTG CAG^CTG
SalI 1226 GTCGAC G^TCGAC
SbfI 121 CCTGCAGG CCTGCA^GG
SfiI 174 GGCCGCTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 232 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 274 CCCGGG CCC^GGG
Sse8387I 121 CCTGCAGG CCTGCA^GG
SspI 1124 AATATT AAT^ATT
StuI 259 AGGCCT AGG^CCT
TaqI 1227 TCGA T^CGA
TthHB8I 1227 TCGA T^CGA
Van91I 1152 CCACAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 436 TCTAGA T^CTAGA
XcmI 1035 CCATCCCTCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 274 CCCGGG C^CCGGG
XmaI 274 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwI 370 GATCC GGATC(4/5)
685 GATCC
AspHI 1130 GTGCTC G[AT]GC[AT]^C
1165 GTGCAC
AvaI 274 CCCGGG C^[CT]CG[GA]G
1133 CTCGGG
BanI 117 GGCGCC G^G[CT][GA]CC
1399 GGCGCC
BanII 82 GAGCCC G[GA]GC[CT]^C
1489 GAGCCC
BbeI 117 GGCGCC GGCGC^C
1399 GGCGCC
BglI 64 GCCAAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
175 GCCGCTGCGGC
BglII 1208 AGATCT A^GATCT
1598 AGATCT
BsaBI 364 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
648 GATGTTAATC
BsaI 24 GAGACC GGTCTC(1/5)
697 GAGACC
BsiEI 107 CGGTCG CG[GA][CT]^CG
182 CGGCCG
BsiHKAI 1130 GTGCTC G[AT]GC[AT]^C
1165 GTGCAC
BsmBI 305 CGTCTC CGTCTC(1/5)
10 GAGACG
BsoBI 274 CCCGGG C^[CT]CG[GA]G
1133 CTCGGG
BsrDI 426 CATTGC GCAATG(2/0)
530 CATTGC
BsrFI 233 GCCGGC [GA]^CCGG[CT]
523 ACCGGT
Cfr10I 233 GCCGGC [GA]^CCGG[CT]
523 ACCGGT
EaeI 62 CGGCCA [CT]^GGCC[GA]
182 CGGCCG
FauI 206 CCCGC CCCGC(4/6)
230 CCCGC
KasI 117 GGCGCC G^GCGCC
1399 GGCGCC
MaeII 269 ACGT A^CGT
891 ACGT
MamI 364 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
648 GATGTTAATC
MspA1I 176 CCGCTG C[AC]G^C[GT]G
197 CAGCTG
NarI 117 GGCGCC GG^CGCC
1399 GGCGCC
PstI 122 CTGCAG CTGCA^G
1557 CTGCAG
SapI 16 GAAGAGC GCTCTTC(1/4)
589 GAAGAGC
SfcI 122 CTGCAG C^T[GA][CT]AG
1557 CTGCAG
SfoI 117 GGCGCC GGC^GCC
1399 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AcyI AluI AocI
AvaII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII EarI
Eco81I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MboI MboII MflI
MnlI MseI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
StyI TfiI Tru9I TseI Tsp509I
TspRI XhoII