Restriction map for
pFN21AE5993
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AvaII AviII AvrII BamHI
BanI BanII BbeI BbrPI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CelII Cfr10I
Cfr13I ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRV FauI FbaI FseI FspI
HaeII HapII HindIII HpaII KasI
KpnI KspI MaeI MaeII MamI
MfeI MluI MroI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tth111I TthHB8I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 859 GTCTAC GT^[AC][GT]AC
AciI 357 CCGC CCGC(-3/-1)
AcyI 692 GACGCC G[GA]^CG[CT]C
AluI 219 AGCT AG^CT
AocI 867 CCTGAGG CC^T[ACGT]AGG
AspHI 416 GTGCTC G[AT]GC[AT]^C
BalI 425 TGGCCA TGG^CCA
BbsI 82 GTCTTC GAAGAC(2/6)
BbvCI 215 CCTCAGC CCTCAGC(-5/-2)
BbvI 364 GCAGC GCAGC(8/12)
BpmI 783 CTCCAG CTGGAG(16/14)
BpuAI 82 GTCTTC GAAGAC(2/6)
BsaHI 692 GACGCC G[GA]^CG[CT]C
BsiHKAI 416 GTGCTC G[AT]GC[AT]^C
BsmFI 751 GGGAC GGGAC(10/14)
BsmI 456 GAATGC GAATGC(1/-1)
BspHI 668 TCATGA T^CATGA
BstXI 230 CCAAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 867 CCTGAGG CC^T[ACGT]AGG
Cac8I 418 GCTCGC GC[ACGT]^[ACGT]GC
CfoI 678 GCGC GCG^C
DraI 895 TTTAAA TTT^AAA
EaeI 425 TGGCCA [CT]^GGCC[GA]
EarI 73 CTCTTC CTCTTC(1/4)
Eco81I 867 CCTGAGG CC^T[ACGT]AGG
EcoRI 25 GAATTC G^AATTC
EcoT14I 230 CCAAGG C^C[AT][AT]GG
EcoT22I 458 ATGCAT ATGCA^T
Fnu4HI 364 GCAGC GC^[ACGT]GC
HgaI 692 GACGC GACGC(5/10)
HhaI 678 GCGC GCG^C
Hin1I 692 GACGCC G[GA]^CG[CT]C
HinP1I 678 GCGC G^CGC
HincII 42 GTTAAC GT[CT]^[GA]AC
HindII 42 GTTAAC GT[CT]^[GA]AC
HpaI 42 GTTAAC GTT^AAC
ItaI 364 GCAGC GC^[ACGT]GC
Ksp632I 73 CTCTTC CTCTTC(1/4)
MluNI 425 TGGCCA TGG^CCA
MscI 425 TGGCCA TGG^CCA
MspA1I 218 CAGCTG C[AC]G^C[GT]G
NsiI 458 ATGCAT ATGCA^T
PflMI 789 CCATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 148 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 218 CAGCTG CAG^CTG
RcaI 668 TCATGA T^CATGA
StyI 230 CCAAGG C^C[AT][AT]GG
TseI 364 GCAGC G^C[AT]GC
Van91I 789 CCATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 25 GAATTC [GA]^AATT[CT]
262 AAATTC
AfaI 171 GTAC GT^AC
768 GTAC
AlwI 66 GATCC GGATC(4/5)
503 GATCC
ApoI 25 GAATTC [GA]^AATT[CT]
262 AAATTC
Asp700I 133 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
477 GAACACTTTC
BmyI 416 GTGCTC G[AGT]GC[ACT]^C
798 GGGCAC
BseRI 536 CTCCTC GAGGAG(10/8)
539 CTCCTC
BsiYI 189 CCTCTCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
789 CCATTTCATGG
BslI 189 CCTCTCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
789 CCATTTCATGG
BsmAI 257 GAGAC GTCTC(1/5)
155 GTCTC
Bsp1286I 416 GTGCTC G[AGT]GC[ACT]^C
798 GGGCAC
BstYI 65 AGATCC [GA]^GATC[CT]
502 AGATCC
MflI 65 AGATCC [GA]^GATC[CT]
502 AGATCC
MwoI 340 GCATCAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
418 GCTCGCCTGGC
NlaIV 116 GGAACC GG[ACGT]^[ACGT]CC
433 GGCTCC
PleI 148 GACTC GAGTC(4/5)
549 GACTC
RsaI 171 GTAC GT^AC
768 GTAC
StuI 701 AGGCCT AGG^CCT
920 AGGCCT
Tsp45I 19 GTGAC ^GT[GC]AC
223 GTCAC
XcmI 230 CCAAGGTGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
785 CCAGCCATTTCATGG
XhoII 65 AGATCC [GA]^GATC[CT]
502 AGATCC
XmnI 133 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
477 GAACACTTTC
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
BsaJI BsrI BssKI BstF5I BstNI
DdeI DpnII EcoRII FokI HaeIII
HinfI HphI MaeIII MboI MboII
MnlI MseI MslI MvaI NdeII
NlaIII PstI Sau3AI ScrFI SfaNI
SfcI TfiI Tru9I Tsp509I TspRI