Restriction map for
pFN21AE5394
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AvaI AvaII AviII BamHI
BanII BbeI BbrPI BcgI BcnI
BfrI BglII BlpI BmyI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV FauI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TthHB8I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 859 GTCTAC GT^[AC][GT]AC
AciI 357 CCGC CCGC(-3/-1)
AcsI 919 AAATTT [GA]^AATT[CT]
AocI 867 CCTGAGG CC^T[ACGT]AGG
ApoI 919 AAATTT [GA]^AATT[CT]
Asp700I 888 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 259 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 429 CCTAGG C^CTAGG
BalI 314 TGGCCA TGG^CCA
BanI 415 GGTGCC G^G[CT][GA]CC
BbsI 742 GTCTTC GAAGAC(2/6)
BbvCI 215 CCTCAGC CCTCAGC(-5/-2)
BciVI 208 GGATAC GTATCC(6/5)
BclI 833 TGATCA T^GATCA
BlnI 429 CCTAGG C^CTAGG
BpmI 783 CTCCAG CTGGAG(16/14)
BpuAI 742 GTCTTC GAAGAC(2/6)
BssKI 120 CCTGG ^CC[ACGT]GG
BstNI 120 CCTGG CC^[AT]GG
BstXI 230 CCAAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 65 AGATCC [GA]^GATC[CT]
Bsu36I 867 CCTGAGG CC^T[ACGT]AGG
Cfr13I 426 GGCCC G^G[ACGT]CC
DraIII 105 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 867 CCTGAGG CC^T[ACGT]AGG
EcoRII 120 CCTGG ^CC[AT]GG
FbaI 833 TGATCA T^GATCA
HindIII 603 AAGCTT A^AGCTT
MflI 65 AGATCC [GA]^GATC[CT]
MluNI 314 TGGCCA TGG^CCA
MscI 314 TGGCCA TGG^CCA
MseI 608 TTAA T^TAA
MspA1I 218 CAGCTG C[AC]G^C[GT]G
MvaI 120 CCTGG CC^[AT]GG
NcoI 794 CCATGG C^CATGG
NdeI 437 CATATG CA^TATG
NspI 280 GCATGT [GA]CATG^[CT]
PflFI 259 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 789 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 148 GACTC GAGTC(4/5)
PshAI 148 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 731 CTGCAG CTGCA^G
PvuII 218 CAGCTG CAG^CTG
Sau96I 426 GGCCC G^G[ACGT]CC
ScrFI 120 CCTGG CC^[ACGT]GG
SfiI 339 GGCCTCAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 574 ACTAGT A^CTAGT
TfiI 585 GATTC G^A[AT]TC
Tru9I 608 TTAA T^TAA
Tth111I 259 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 789 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 785 CCAGCCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 65 AGATCC [GA]^GATC[CT]
XmnI 888 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
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AlwI 66 GATCC GGATC(4/5)
138 GATCC
BbvI 364 GCAGC GCAGC(8/12)
774 GCAGC
BglI 340 GCCTCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
418 GCCTGTCTGGC
BsrI 47 CCAGT ACTGG(1/-1)
407 CCAGT
BstF5I 837 CATCC GGATG(2/0)
125 GGATG
DsaI 317 CCACGG C^C[GA][CT]GG
794 CCATGG
EaeI 314 TGGCCA [CT]^GGCC[GA]
404 CGGCCA
Fnu4HI 364 GCAGC GC^[ACGT]GC
774 GCAGC
FokI 837 CATCC GGATG(9/13)
125 GGATG
HinfI 148 GACTC G^A[ACGT]TC
585 GATTC
ItaI 364 GCAGC GC^[ACGT]GC
774 GCAGC
SfaNI 675 GATGC GCATC(5/9)
814 GCATC
TseI 364 GCAGC G^C[AT]GC
774 GCAGC
Tsp45I 19 GTGAC ^GT[GC]AC
223 GTCAC
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AfaI AluI BfaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsmI
DdeI DpnII EcoT14I EcoT22I HaeIII
HphI MaeI MaeIII MboI MboII
MnlI MslI MwoI NdeII NlaIII
NlaIV NsiI RsaI Sau3AI SfcI
StyI Tsp509I TspRI