Restriction map for
pFN21AE5996
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccII AccIII AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AvaII AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI BstBI
BstEII BstPI BstUI BstYI Bsu36I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DpnII DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MamI
MboI MfeI MflI MluI MroI
MseI MspI MunI MvnI NaeI
NarI NciI NdeI NdeII NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
RcaI RsrII SacI SacII SalI
SapI Sau3AI Sau96I SbfI ScaI
SexAI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I TthHB8I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 691 GTATAC GT^[AC][GT]AC
AciI 357 CCGC CCGC(-3/-1)
AclI 81 AACGTT AA^CGTT
AspHI 416 GTGCTC G[AT]GC[AT]^C
AspI 259 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 425 TGGCCA TGG^CCA
BanI 813 GGCACC G^G[CT][GA]CC
BbsI 256 GAAGAC GAAGAC(2/6)
BglI 340 GCCTCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 416 GTGCTC G[AGT]GC[ACT]^C
BpmI 783 CTCCAG CTGGAG(16/14)
BpuAI 256 GAAGAC GAAGAC(2/6)
BsiHKAI 416 GTGCTC G[AT]GC[AT]^C
BsiYI 468 CCACACTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 468 CCACACTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 416 GTGCTC G[AGT]GC[ACT]^C
BspMI 63 GCAGGT ACCTGC(4/8)
BsrDI 729 CATTGC GCAATG(2/0)
Bst1107I 691 GTATAC GTA^TAC
BstAPI 280 GCATGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 230 CCATCGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 691 GTATAC GTA^TAC
Cac8I 578 GCGAGC GC[ACGT]^[ACGT]GC
DsaI 794 CCATGG C^C[GA][CT]GG
EaeI 425 TGGCCA [CT]^GGCC[GA]
EcoRV 639 GATATC GAT^ATC
EcoT14I 794 CCATGG C^C[AT][AT]GG
EcoT22I 278 ATGCAT ATGCA^T
HphI 662 TCACC GGTGA(8/7)
MaeII 82 ACGT A^CGT
MluNI 425 TGGCCA TGG^CCA
MscI 425 TGGCCA TGG^CCA
MspA1I 218 CAGCTG C[AC]G^C[GT]G
NcoI 794 CCATGG C^CATGG
NsiI 278 ATGCAT ATGCA^T
NspI 280 GCATGT [GA]CATG^[CT]
PflFI 259 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 52 GACTC GAGTC(4/5)
Psp1406I 81 AACGTT AA^CGTT
PstI 61 CTGCAG CTGCA^G
PvuII 218 CAGCTG CAG^CTG
SfiI 339 GGCCTCAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI 794 CCATGG C^C[AT][AT]GG
Tth111I 259 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 785 CCAGTCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AfaI 171 GTAC GT^AC
768 GTAC
BbvI 364 GCAGC GCAGC(8/12)
286 GCTGC
BfaI 37 CTAG C^TAG
196 CTAG
BsaJI 120 CCTGGG C^C[ACGT][ACGT]GG
794 CCATGG
BsmFI 476 GGGAC GGGAC(10/14)
751 GGGAC
BsmI 456 GAATGC GAATGC(1/-1)
892 GCATTC
BsrI 472 ACTGG ACTGG(1/-1)
785 CCAGT
BssKI 120 CCTGG ^CC[ACGT]GG
627 CCTGG
BstNI 120 CCTGG CC^[AT]GG
627 CCTGG
EarI 213 CTCTTC CTCTTC(1/4)
885 GAAGAG
EcoRII 120 CCTGG ^CC[AT]GG
627 CCTGG
Fnu4HI 286 GCTGC GC^[ACGT]GC
364 GCAGC
ItaI 286 GCTGC GC^[ACGT]GC
364 GCAGC
Ksp632I 213 CTCTTC CTCTTC(1/4)
885 GAAGAG
MaeI 37 CTAG C^TAG
196 CTAG
MvaI 120 CCTGG CC^[AT]GG
627 CCTGG
RsaI 171 GTAC GT^AC
768 GTAC
ScrFI 120 CCTGG CC^[ACGT]GG
627 CCTGG
SfaNI 675 GATGC GCATC(5/9)
740 GCATC
TfiI 148 GATTC G^A[AT]TC
245 GATTC
TseI 286 GCTGC G^C[AT]GC
364 GCAGC
Tsp45I 223 GTCAC ^GT[GC]AC
788 GTCAC
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# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
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AluI BsmAI BstF5I DdeI FokI
HaeIII HinfI MaeIII MboII MnlI
MslI MwoI NlaIII NlaIV SfcI
Tsp509I TspRI