Restriction map for
pFN21AE6008
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AlwI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BcgI BcnI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmBI BsmFI BsoBI
Bsp1286I BspDI BspEI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRV FauI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MamI MfeI MflI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
TaqI Tth111I TthHB8I Van91I XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AciI 357 CCGC CCGC(-3/-1)
AcsI 25 GAATTC [GA]^AATT[CT]
AflII 670 CTTAAG C^TTAAG
AluI 219 AGCT AG^CT
AlwNI 276 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 867 CCTGAGG CC^T[ACGT]AGG
ApoI 25 GAATTC [GA]^AATT[CT]
AseI 135 ATTAAT AT^TAAT
AsnI 135 ATTAAT AT^TAAT
Asp700I 888 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 407 CACGTG CAC^GTG
BbsI 256 GAAGAC GAAGAC(2/6)
BbvCI 215 CCTCAGC CCTCAGC(-5/-2)
BciVI 691 GGATAC GTATCC(6/5)
BfaI 928 CTAG C^TAG
BfrI 670 CTTAAG C^TTAAG
BpuAI 256 GAAGAC GAAGAC(2/6)
BsaAI 407 CACGTG [CT]AC^GT[GA]
BseRI 213 CTCCTC GAGGAG(10/8)
BsgI 305 GTGCAG GTGCAG(16/14)
BsmAI 192 GTCTC GTCTC(1/5)
Bsp1407I 767 TGTACA T^GTACA
BspHI 896 TCATGA T^CATGA
BspMI 556 ACCTGC ACCTGC(4/8)
BsrDI 729 CATTGC GCAATG(2/0)
BsrGI 767 TGTACA T^GTACA
BssKI 120 CCTGG ^CC[ACGT]GG
BstAPI 307 GCAGTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 120 CCTGG CC^[AT]GG
Bsu36I 867 CCTGAGG CC^T[ACGT]AGG
Cac8I 199 GCAGGC GC[ACGT]^[ACGT]GC
DsaI 794 CCATGG C^C[GA][CT]GG
Eco81I 867 CCTGAGG CC^T[ACGT]AGG
EcoRI 25 GAATTC G^AATTC
EcoRII 120 CCTGG ^CC[AT]GG
EcoT14I 794 CCATGG C^C[AT][AT]GG
EcoT22I 890 ATGCAT ATGCA^T
HaeIII 348 GGCC GG^CC
MaeI 928 CTAG C^TAG
MspA1I 218 CAGCTG C[AC]G^C[GT]G
MvaI 120 CCTGG CC^[AT]GG
NcoI 794 CCATGG C^CATGG
NsiI 890 ATGCAT ATGCA^T
NspI 500 GCATGT [GA]CATG^[CT]
PleI 148 GACTC GAGTC(4/5)
PmaCI 407 CACGTG CAC^GTG
PmlI 407 CACGTG CAC^GTG
PshBI 135 ATTAAT AT^TAAT
PvuII 218 CAGCTG CAG^CTG
RcaI 896 TCATGA T^CATGA
ScrFI 120 CCTGG CC^[ACGT]GG
SfaNI 814 GCATC GCATC(5/9)
SmlI 670 CTTAAG C^T[CT][GA]AG
SspBI 767 TGTACA T^GTACA
SspI 920 AATATT AAT^ATT
StyI 794 CCATGG C^C[AT][AT]GG
XbaI 927 TCTAGA T^CTAGA
XcmI 785 CCAGTCATTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 888 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
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AfaI 171 GTAC GT^AC
768 GTAC
AflIII 406 ACACGT A^C[GA][CT]GT
408 ACGTGT
BbvI 364 GCAGC GCAGC(8/12)
286 GCTGC
BsaJI 120 CCTGGG C^C[ACGT][ACGT]GG
794 CCATGG
Fnu4HI 286 GCTGC GC^[ACGT]GC
364 GCAGC
ItaI 286 GCTGC GC^[ACGT]GC
364 GCAGC
MaeII 408 ACGT A^CGT
881 ACGT
MboII 256 GAAGA GAAGA(8/7)
885 GAAGA
NlaIV 66 GGTTCC GG[ACGT]^[ACGT]CC
116 GGAACC
RsaI 171 GTAC GT^AC
768 GTAC
TseI 286 GCTGC G^C[AT]GC
364 GCAGC
Tsp45I 223 GTCAC ^GT[GC]AC
530 GTGAC
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
BclI BsmI BsrI BstF5I DdeI
DpnII FbaI FokI HinfI HphI
MaeIII MboI MnlI MseI MslI
MwoI NdeII NlaIII Sau3AI SfcI
TfiI Tru9I Tsp509I TspRI