Restriction map for
pFN21AE6087
##################################
# NO CUT ENZYMES ( 177 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRV
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MaeI MaeII MamI MflI
MluI MluNI MroI MscI MseI
MspA1I MspI MvnI MwoI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TfiI Tru9I
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AciI 447 GCGG CCGC(-3/-1)
AocI 838 CCTGAGG CC^T[ACGT]AGG
AvaII 386 GGACC G^G[AT]CC
BanI 934 GGTGCC G^G[CT][GA]CC
BanII 166 GGGCTC G[GA]GC[CT]^C
BbsI 3 GAAGAC GAAGAC(2/6)
BbvI 326 GCTGC GCAGC(8/12)
BclI 617 TGATCA T^GATCA
BpuAI 3 GAAGAC GAAGAC(2/6)
BsiYI 402 CCACTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 402 CCACTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BssSI 480 CTCGTG CACGAG(-5/-1)
BstF5I 725 CATCC GGATG(2/0)
BstXI 275 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 838 CCTGAGG CC^T[ACGT]AGG
Cac8I 447 GCGGGC GC[ACGT]^[ACGT]GC
DraIII 403 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 389 CCATGG C^C[GA][CT]GG
Eco81I 838 CCTGAGG CC^T[ACGT]AGG
EcoRI 70 GAATTC G^AATTC
EcoT14I 389 CCATGG C^C[AT][AT]GG
EcoT22I 40 ATGCAT ATGCA^T
FauI 447 GCGGG CCCGC(4/6)
FbaI 617 TGATCA T^GATCA
Fnu4HI 326 GCTGC GC^[ACGT]GC
FokI 725 CATCC GGATG(9/13)
HinfI 86 GACTC G^A[ACGT]TC
ItaI 326 GCTGC GC^[ACGT]GC
NcoI 389 CCATGG C^CATGG
NsiI 40 ATGCAT ATGCA^T
PflMI 402 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 86 GACTC GAGTC(4/5)
SapI 537 GCTCTTC GCTCTTC(1/4)
SmlI 912 CTTGAG C^T[CT][GA]AG
StyI 389 CCATGG C^C[AT][AT]GG
TaqI 398 TCGA T^CGA
TseI 326 GCTGC G^C[AT]GC
TthHB8I 398 TCGA T^CGA
Van91I 402 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AcsI 70 GAATTC [GA]^AATT[CT]
962 AAATTT
ApoI 70 GAATTC [GA]^AATT[CT]
962 AAATTT
BmyI 166 GGGCTC G[AGT]GC[ACT]^C
935 GTGCCC
BseRI 115 CTCCTC GAGGAG(10/8)
825 CTCCTC
Bsp1286I 166 GGGCTC G[AGT]GC[ACT]^C
935 GTGCCC
EarI 220 CTCTTC CTCTTC(1/4)
538 CTCTTC
Ksp632I 220 CTCTTC CTCTTC(1/4)
538 CTCTTC
MfeI 532 CAATTG C^AATTG
776 CAATTG
MunI 532 CAATTG C^AATTG
776 CAATTG
SfcI 803 CTGTAG C^T[GA][CT]AG
906 CTACAG
SspI 289 AATATT AAT^ATT
496 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AfaI AluI Asp700I BsaJI BsmFI
BssKI BstNI Cfr13I DdeI DpnII
EcoRII HaeIII HphI MaeIII MboI
MboII MnlI MslI MvaI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI Tsp45I Tsp509I TspRI
XmnI