Restriction map for
pFN21AE6001
##################################
# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BamHI
BanII BbeI BbrPI BcgI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
BanI 757 GGCACC G^G[CT][GA]CC
BbsI 935 GAAGAC GAAGAC(2/6)
BbvCI 186 CCTCAGC CCTCAGC(-5/-2)
BbvI 292 GCAGC GCAGC(8/12)
BciVI 646 GTATCC GTATCC(6/5)
BcnI 699 CCGGG CC^[GC]GG
BglI 346 GCCATCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 935 GAAGAC GAAGAC(2/6)
BseRI 545 CTCCTC GAGGAG(10/8)
BsgI 290 GTGCAG GTGCAG(16/14)
BsmAI 782 GAGAC GTCTC(1/5)
BsmFI 569 GGGAC GGGAC(10/14)
BstBI 473 TTCGAA TT^CGAA
BstEII 840 GGTGACC G^GT[ACGT]ACC
BstNI 279 CCAGG CC^[AT]GG
BstPI 840 GGTGACC G^GT[ACGT]ACC
BstXI 684 CCACTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 680 AGATCC [GA]^GATC[CT]
CfoI 722 GCGC GCG^C
DraII 425 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 147 CCGTGG C^C[GA][CT]GG
EagI 371 CGGCCG C^GGCCG
EclXI 371 CGGCCG C^GGCCG
Eco52I 371 CGGCCG C^GGCCG
EcoO109I 425 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 840 GGTGACC G^GT[ACGT]ACC
EcoRII 279 CCAGG ^CC[AT]GG
EcoT14I 200 CCTTGG C^C[AT][AT]GG
FauI 419 CCCGC CCCGC(4/6)
Fnu4HI 292 GCAGC GC^[ACGT]GC
HapII 699 CCGG C^CGG
HhaI 722 GCGC GCG^C
HinP1I 722 GCGC G^CGC
HinfI 855 GAATC G^A[ACGT]TC
HpaII 699 CCGG C^CGG
ItaI 292 GCAGC GC^[ACGT]GC
MaeII 446 ACGT A^CGT
MflI 680 AGATCC [GA]^GATC[CT]
MseI 553 TTAA T^TAA
MspA1I 420 CCGCTG C[AC]G^C[GT]G
MspI 699 CCGG C^CGG
MvaI 279 CCAGG CC^[AT]GG
NciI 699 CCGGG CC^[GC]GG
NspV 473 TTCGAA TT^CGAA
PstI 676 CTGCAG CTGCA^G
PvuI 153 CGATCG CGAT^CG
SfaNI 240 GATGC GCATC(5/9)
SfiI 345 GGCCATCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 473 TTCGAA TT^CGAA
StyI 200 CCTTGG C^C[AT][AT]GG
TfiI 855 GAATC G^A[AT]TC
Tru9I 553 TTAA T^TAA
TseI 292 GCAGC G^C[AT]GC
Tsp509I 13 AATT ^AATT
XhoII 680 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 177 GTAC GT^AC
774 GTAC
AluI 294 AGCT AG^CT
574 AGCT
AlwNI 238 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
382 CAGCCCCTG
AspHI 259 GAGCAC G[AT]GC[AT]^C
826 GTGCTC
BalI 344 TGGCCA TGG^CCA
668 TGGCCA
BmyI 259 GAGCAC G[AGT]GC[ACT]^C
826 GTGCTC
BpmI 61 CTCCAG CTGGAG(16/14)
692 CTGGAG
BsiEI 153 CGATCG CG[GA][CT]^CG
371 CGGCCG
BsiHKAI 259 GAGCAC G[AT]GC[AT]^C
826 GTGCTC
Bsp1286I 259 GAGCAC G[AGT]GC[ACT]^C
826 GTGCTC
BspMI 718 ACCTGC ACCTGC(4/8)
776 ACCTGC
BssKI 279 CCAGG ^CC[ACGT]GG
699 CCGGG
BstF5I 231 CATCC GGATG(2/0)
672 CATCC
EarI 79 CTCTTC CTCTTC(1/4)
307 CTCTTC
FokI 231 CATCC GGATG(9/13)
672 CATCC
Ksp632I 79 CTCTTC CTCTTC(1/4)
307 CTCTTC
MluNI 344 TGGCCA TGG^CCA
668 TGGCCA
MscI 344 TGGCCA TGG^CCA
668 TGGCCA
RsaI 177 GTAC GT^AC
774 GTAC
ScrFI 279 CCAGG CC^[ACGT]GG
699 CCGGG
SfcI 676 CTGCAG C^T[GA][CT]AG
867 CTATAG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AlwI BfaI BsaJI BsiYI
BslI BsrI Cac8I Cfr13I DdeI
DpnII EaeI HaeIII HphI MaeI
MaeIII MboI MboII MnlI MslI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I TaqI Tsp45I TspRI TthHB8I