Restriction map for
pFN21AE5994
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BglII BlnI BlpI
Bpu1102I BsaAI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HapII HhaI HinP1I
HincII HindII HpaI HpaII KasI
KpnI Ksp632I KspI MaeII MfeI
MluI MroI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 31 GAATTC [GA]^AATT[CT]
AcyI 895 GACGCC G[GA]^CG[CT]C
AflII 873 CTTAAG C^TTAAG
AflIII 718 ACATGT A^C[GA][CT]GT
AlwI 240 GATCC GGATC(4/5)
AlwNI 414 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 31 GAATTC [GA]^AATT[CT]
Asp700I 31 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 811 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 54 GGACC G^G[AT]CC
BanII 758 GAGCCC G[GA]GC[CT]^C
BfrI 873 CTTAAG C^TTAAG
BglI 247 GCCACATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 758 GAGCCC G[AGT]GC[ACT]^C
BsaBI 588 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 895 GACGCC G[GA]^CG[CT]C
BsgI 320 GTGCAG GTGCAG(16/14)
BsmAI 918 GAGAC GTCTC(1/5)
BsmFI 930 GTCCC GGGAC(10/14)
Bsp1286I 758 GAGCCC G[AGT]GC[ACT]^C
BspLU11I 718 ACATGT A^CATGT
BsrI 297 CCAGT ACTGG(1/-1)
BstAPI 326 GCACAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 239 AGATCC [GA]^GATC[CT]
DraIII 327 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 398 CCGTGG C^C[GA][CT]GG
EcoRI 31 GAATTC G^AATTC
HgaI 895 GACGC GACGC(5/10)
Hin1I 895 GACGCC G[GA]^CG[CT]C
HindIII 556 AAGCTT A^AGCTT
MamI 588 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 239 AGATCC [GA]^GATC[CT]
NspI 718 ACATGT [GA]CATG^[CT]
PflFI 811 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 920 GACTC GAGTC(4/5)
PstI 564 CTGCAG CTGCA^G
SfaNI 208 GATGC GCATC(5/9)
SmlI 873 CTTAAG C^T[CT][GA]AG
TaqI 584 TCGA T^CGA
Tth111I 811 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 584 TCGA T^CGA
XhoII 239 AGATCC [GA]^GATC[CT]
XmnI 31 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
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AfaI 177 GTAC GT^AC
476 GTAC
AluI 557 AGCT AG^CT
871 AGCT
BbvI 292 GCTGC GCAGC(8/12)
370 GCTGC
BsaJI 398 CCGTGG C^C[ACGT][ACGT]GG
412 CCCAGG
Fnu4HI 292 GCTGC GC^[ACGT]GC
370 GCTGC
ItaI 292 GCTGC GC^[ACGT]GC
370 GCTGC
MseI 142 TTAA T^TAA
874 TTAA
RsaI 177 GTAC GT^AC
476 GTAC
SfcI 564 CTGCAG C^T[GA][CT]AG
867 CTACAG
TfiI 72 GATTC G^A[AT]TC
408 GAATC
Tru9I 142 TTAA T^TAA
874 TTAA
TseI 292 GCTGC G^C[AT]GC
370 GCTGC
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
BalI BbsI BfaI BpmI BpuAI
BsiYI BslI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EaeI
EcoRII FokI HaeIII HinfI HphI
MaeI MaeIII MboI MboII MluNI
MnlI MscI MslI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI Tsp45I Tsp509I TspRI