Restriction map for
pFN21AE5473
##################################
# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspHI
AspI AvaI AvaII AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BcnI BfrI BglI BglII
BlnI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsoBI BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI BstYI Bsu36I Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoT22I FauI FseI FspI HaeII
HapII HgaI Hin1I HindIII HpaI
HpaII KasI KspI MaeII MamI
MfeI MflI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI Sau96I SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI TaqI Tsp45I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 445 GGTACC G^GTACC
AcsI 76 GAATTC [GA]^AATT[CT]
AlwI 802 GGATC GGATC(4/5)
ApoI 76 GAATTC [GA]^AATT[CT]
Asp700I 194 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 445 GGTACC G^GTACC
BalI 290 TGGCCA TGG^CCA
BanI 445 GGTACC G^G[CT][GA]CC
BanII 338 GGGCTC G[GA]GC[CT]^C
BbsI 743 GAAGAC GAAGAC(2/6)
BclI 884 TGATCA T^GATCA
BlpI 340 GCTCAGC GC^T[ACGT]AGC
BmyI 338 GGGCTC G[AGT]GC[ACT]^C
BpmI 669 CTCCAG CTGGAG(16/14)
Bpu1102I 340 GCTCAGC GC^T[ACGT]AGC
BpuAI 743 GAAGAC GAAGAC(2/6)
BsmAI 301 GTCTC GTCTC(1/5)
BsmFI 854 GGGAC GGGAC(10/14)
Bsp1286I 338 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 610 TGTACA T^GTACA
BspHI 15 TCATGA T^CATGA
BsrDI 431 CATTGC GCAATG(2/0)
BsrGI 610 TGTACA T^GTACA
BstXI 281 CCAAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 340 GCTCAGC GC^T[ACGT]AGC
CfoI 315 GCGC GCG^C
DrdI 616 GACACCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 290 TGGCCA [CT]^GGCC[GA]
EarI 793 CTCTTC CTCTTC(1/4)
EcoRI 76 GAATTC G^AATTC
EcoRV 931 GATATC GAT^ATC
FbaI 884 TGATCA T^GATCA
HhaI 315 GCGC GCG^C
HinP1I 315 GCGC G^CGC
HincII 700 GTCAAC GT[CT]^[GA]AC
HindII 700 GTCAAC GT[CT]^[GA]AC
HphI 32 TCACC GGTGA(8/7)
KpnI 445 GGTACC GGTAC^C
Ksp632I 793 CTCTTC CTCTTC(1/4)
MaeIII 425 GTAAC ^GT[ACGT]AC
MluNI 290 TGGCCA TGG^CCA
MscI 290 TGGCCA TGG^CCA
MseI 187 TTAA T^TAA
MspA1I 343 CAGCTG C[AC]G^C[GT]G
NcoI 395 CCATGG C^CATGG
NdeI 401 CATATG CA^TATG
PvuII 343 CAGCTG CAG^CTG
RcaI 15 TCATGA T^CATGA
SapI 792 GCTCTTC GCTCTTC(1/4)
ScaI 663 AGTACT AGT^ACT
SfcI 912 CTATAG C^T[GA][CT]AG
SspBI 610 TGTACA T^GTACA
Tru9I 187 TTAA T^TAA
XmnI 194 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BfaI 830 CTAG C^TAG
847 CTAG
BsiYI 845 CCCTAGAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
846 CCTAGAGAGGG
BslI 845 CCCTAGAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
846 CCTAGAGAGGG
Bst1107I 261 GTATAC GTA^TAC
273 GTATAC
BstZ17I 261 GTATAC GTA^TAC
273 GTATAC
Cac8I 481 GCTGGC GC[ACGT]^[ACGT]GC
539 GCCTGC
DpnII 803 GATC ^GATC
885 GATC
DsaI 38 CCGTGG C^C[GA][CT]GG
395 CCATGG
MaeI 830 CTAG C^TAG
847 CTAG
MboI 803 GATC ^GATC
885 GATC
MwoI 391 GCAGCCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
517 GCCCATACTGC
NdeII 803 GATC ^GATC
885 GATC
NlaIV 445 GGTACC GG[ACGT]^[ACGT]CC
484 GGCTCC
Sau3AI 803 GATC ^GATC
885 GATC
SfaNI 141 GATGC GCATC(5/9)
583 GATGC
StuI 826 AGGCCT AGG^CCT
941 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AccI AciI AfaI AluI BbvI
BsaJI BsmI BsrI BssKI BstF5I
BstNI DdeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI ItaI MboII
MnlI MslI MvaI NlaIII RsaI
ScrFI StyI TfiI TseI Tsp509I
TspRI