Restriction map for
pFN21AE5466
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AlwI AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BcnI BglI BlnI
BlpI Bpu1102I BsaAI BsaHI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaI HpaII
KasI KpnI KspI MaeII MfeI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TfiI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 380 GTCGAC GT^[AC][GT]AC
AcsI 619 GAATTC [GA]^AATT[CT]
AfaI 774 GTAC GT^AC
AflII 703 CTTAAG C^TTAAG
AocI 777 CCTGAGG CC^T[ACGT]AGG
ApoI 619 GAATTC [GA]^AATT[CT]
Asp700I 680 GAATGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 371 TGGCCA TGG^CCA
BanII 438 GGGCTC G[GA]GC[CT]^C
BbsI 88 GTCTTC GAAGAC(2/6)
BbvCI 221 CCTCAGC CCTCAGC(-5/-2)
BfaI 457 CTAG C^TAG
BfrI 703 CTTAAG C^TTAAG
BglII 587 AGATCT A^GATCT
BmyI 438 GGGCTC G[AGT]GC[ACT]^C
BpmI 282 CTCCAG CTGGAG(16/14)
BpuAI 88 GTCTTC GAAGAC(2/6)
BsaBI 66 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 725 GGTCTC GGTCTC(1/5)
BseRI 219 CTCCTC GAGGAG(10/8)
BsmAI 726 GTCTC GTCTC(1/5)
BsmI 680 GAATGC GAATGC(1/-1)
Bsp1286I 438 GGGCTC G[AGT]GC[ACT]^C
BsrDI 228 CATTGC GCAATG(2/0)
BstXI 236 CCAGGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 587 AGATCT [GA]^GATC[CT]
Bsu36I 777 CCTGAGG CC^T[ACGT]AGG
Cac8I 243 GCTGGC GC[ACGT]^[ACGT]GC
DraII 56 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 350 CCATGG C^C[GA][CT]GG
EaeI 371 TGGCCA [CT]^GGCC[GA]
Eco81I 777 CCTGAGG CC^T[ACGT]AGG
EcoO109I 56 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 619 GAATTC G^AATTC
EcoT14I 350 CCATGG C^C[AT][AT]GG
FauI 722 GCGGG CCCGC(4/6)
MaeI 457 CTAG C^TAG
MamI 66 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 587 AGATCT [GA]^GATC[CT]
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MwoI 346 GCAGCCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 350 CCATGG C^CATGG
PpuMI 56 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 774 GTAC GT^AC
SalI 380 GTCGAC G^TCGAC
SapI 594 GCTCTTC GCTCTTC(1/4)
SexAI 104 ACCTGGT A^CC[AT]GGT
SmlI 703 CTTAAG C^T[CT][GA]AG
SspI 124 AATATT AAT^ATT
StyI 350 CCATGG C^C[AT][AT]GG
TaqI 381 TCGA T^CGA
Tsp45I 25 GTGAC ^GT[GC]AC
TspRI 582 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 381 TCGA T^CGA
XcmI 791 CCAGCTACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 587 AGATCT [GA]^GATC[CT]
XmnI 680 GAATGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AvaII 57 GGACC G^G[AT]CC
804 GGACC
BbvI 346 GCAGC GCAGC(8/12)
895 GCTGC
BspMI 718 ACCTGC ACCTGC(4/8)
414 GCAGGT
BsrI 736 ACTGG ACTGG(1/-1)
297 CCAGT
EarI 595 CTCTTC CTCTTC(1/4)
748 CTCTTC
HincII 205 GTTGAC GT[CT]^[GA]AC
380 GTCGAC
HindII 205 GTTGAC GT[CT]^[GA]AC
380 GTCGAC
Ksp632I 595 CTCTTC CTCTTC(1/4)
748 CTCTTC
MaeIII 25 GTGAC ^GT[ACGT]AC
500 GTTAC
MspA1I 285 CAGCTG C[AC]G^C[GT]G
689 CAGCTG
NlaIV 57 GGACCC GG[ACGT]^[ACGT]CC
508 GGTTCC
PvuII 285 CAGCTG CAG^CTG
689 CAGCTG
SfcI 393 CTATAG C^T[GA][CT]AG
867 CTACAG
TseI 346 GCAGC G^C[AT]GC
895 GCTGC
Tsp509I 620 AATT ^AATT
912 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AluI BsaJI BsiYI BslI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HphI ItaI MboI MboII MnlI
MseI MslI MvaI NdeII NlaIII
Sau3AI Sau96I ScrFI SfaNI Tru9I