Restriction map for
pFN21AE6027
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfrI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraIII DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
FspI HaeII HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MaeII MamI
MfeI MluI MroI MspI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AfaI 792 GTAC GT^AC
AlwI 536 GGATC GGATC(4/5)
BalI 362 TGGCCA TGG^CCA
BclI 611 TGATCA T^GATCA
BfaI 809 CTAG C^TAG
BglI 364 GCCATGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaJI 339 CCTGGG C^C[ACGT][ACGT]GG
BseRI 55 CTCCTC GAGGAG(10/8)
BsiYI 335 CCTTCCTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 335 CCTTCCTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 343 GGGAC GGGAC(10/14)
BsmI 619 GCATTC GAATGC(1/-1)
BspMI 469 GCAGGT ACCTGC(4/8)
BsrDI 684 CATTGC GCAATG(2/0)
BssKI 339 CCTGG ^CC[ACGT]GG
BssSI 421 CTCGTG CACGAG(-5/-1)
BstNI 339 CCTGG CC^[AT]GG
Cac8I 716 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 798 GCGC GCG^C
Cfr13I 915 GGCCC G^G[ACGT]CC
DraI 891 TTTAAA TTT^AAA
DraII 914 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 829 GACAAGGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 362 TGGCCA [CT]^GGCC[GA]
EcoO109I 914 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 339 CCTGG ^CC[AT]GG
FauI 561 CCCGC CCCGC(4/6)
FbaI 611 TGATCA T^GATCA
HhaI 798 GCGC GCG^C
HinP1I 798 GCGC G^CGC
HinfI 92 GAGTC G^A[ACGT]TC
MaeI 809 CTAG C^TAG
MluNI 362 TGGCCA TGG^CCA
MscI 362 TGGCCA TGG^CCA
MspA1I 642 CAGCTG C[AC]G^C[GT]G
MvaI 339 CCTGG CC^[AT]GG
MwoI 364 GCCATGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 374 CATATG CA^TATG
PleI 92 GAGTC GAGTC(4/5)
PvuII 642 CAGCTG CAG^CTG
RsaI 792 GTAC GT^AC
Sau96I 915 GGCCC G^G[ACGT]CC
ScrFI 339 CCTGG CC^[ACGT]GG
SfcI 885 CTATAG C^T[GA][CT]AG
SmlI 624 CTCAAG C^T[CT][GA]AG
TaqI 21 TCGA T^CGA
TspRI 566 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 21 TCGA T^CGA
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# TWO CUT ENZYMES ( 15 enzymes) #
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AciI 381 CCGC CCGC(-3/-1)
562 CCGC
AcsI 49 GAATTT [GA]^AATT[CT]
434 GAATTT
ApoI 49 GAATTT [GA]^AATT[CT]
434 GAATTT
BglII 81 AGATCT A^GATCT
926 AGATCT
BsrI 315 CCAGT ACTGG(1/-1)
415 CCAGT
BstXI 254 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
594 CCACTTCAATGG
BstYI 81 AGATCT [GA]^GATC[CT]
926 AGATCT
EarI 237 CTCTTC CTCTTC(1/4)
784 CTCTTC
HphI 264 GGTGA GGTGA(8/7)
761 TCACC
Ksp632I 237 CTCTTC CTCTTC(1/4)
784 CTCTTC
MflI 81 AGATCT [GA]^GATC[CT]
926 AGATCT
MseI 164 TTAA T^TAA
892 TTAA
NlaIV 331 GGCTCC GG[ACGT]^[ACGT]CC
398 GGAACC
Tru9I 164 TTAA T^TAA
892 TTAA
XhoII 81 AGATCT [GA]^GATC[CT]
926 AGATCT
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# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AluI BbvI BsmAI BstF5I DdeI
DpnII Fnu4HI FokI HaeIII ItaI
MaeIII MboI MboII MnlI MslI
NdeII NlaIII Sau3AI SfaNI TseI
Tsp45I Tsp509I