Restriction map for
pFN21AE6080
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BanI
BanII BbeI BbrPI BbsI BbvI
BcgI BciVI BcnI BfrI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiHKAI BsiWI
BsmAI BsmBI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspLU11I BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstZ17I CelII CfoI ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FokI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NsiI NspI
NspV PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI TseI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AocI 675 CCTAAGG CC^T[ACGT]AGG
BamHI 680 GGATCC G^GATCC
BbvCI 186 CCTCAGC CCTCAGC(-5/-2)
BclI 323 TGATCA T^GATCA
BfaI 343 CTAG C^TAG
BglI 346 GCCTCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 219 CTCCTC GAGGAG(10/8)
BsiEI 360 CGGTCG CG[GA][CT]^CG
BsiYI 76 CCGTGCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 76 CCGTGCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 383 GTCCC GGGAC(10/14)
BsmI 422 GCATTC GAATGC(1/-1)
BspHI 320 TCATGA T^CATGA
BsrBI 684 CCGCTC CCGCTC(-3/-3)
BsrFI 56 ACCGGC [GA]^CCGG[CT]
BstYI 680 GGATCC [GA]^GATC[CT]
Bsu36I 675 CCTAAGG CC^T[ACGT]AGG
Cac8I 607 GCTGGC GC[ACGT]^[ACGT]GC
Cfr10I 56 ACCGGC [GA]^CCGG[CT]
DraI 951 TTTAAA TTT^AAA
DraII 71 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 327 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 350 CCATGG C^C[GA][CT]GG
EaeI 58 CGGCCA [CT]^GGCC[GA]
Eco81I 675 CCTAAGG CC^T[ACGT]AGG
EcoO109I 71 AGGCCCC [GA]G^G[ACGT]CC[CT]
FbaI 323 TGATCA T^GATCA
Fnu4HI 667 GCCGC GC^[ACGT]GC
HapII 57 CCGG C^CGG
HincII 205 GTTGAC GT[CT]^[GA]AC
HindII 205 GTTGAC GT[CT]^[GA]AC
HpaII 57 CCGG C^CGG
ItaI 667 GCCGC GC^[ACGT]GC
MaeI 343 CTAG C^TAG
MflI 680 GGATCC [GA]^GATC[CT]
MspI 57 CCGG C^CGG
MwoI 346 GCCTCCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 350 CCATGG C^CATGG
NdeI 730 CATATG CA^TATG
PacI 517 TTAATTAA TTAAT^TAA
RcaI 320 TCATGA T^CATGA
SfaNI 895 GATGC GCATC(5/9)
TaqI 159 TCGA T^CGA
TthHB8I 159 TCGA T^CGA
XhoII 680 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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AciI 668 CCGC CCGC(-3/-1)
684 CCGC
AcsI 249 GAATTT [GA]^AATT[CT]
587 AAATTC
AfaI 177 GTAC GT^AC
774 GTAC
ApoI 249 GAATTT [GA]^AATT[CT]
587 AAATTC
BsrI 300 ACTGG ACTGG(1/-1)
429 ACTGG
BssKI 195 CCTGG ^CC[ACGT]GG
459 CCTGG
BstNI 195 CCTGG CC^[AT]GG
459 CCTGG
Cfr13I 72 GGCCC G^G[ACGT]CC
508 GGCCC
EcoRII 195 CCTGG ^CC[AT]GG
459 CCTGG
HinfI 161 GACTC G^A[ACGT]TC
416 GAGTC
MvaI 195 CCTGG CC^[AT]GG
459 CCTGG
PleI 161 GACTC GAGTC(4/5)
416 GAGTC
RsaI 177 GTAC GT^AC
774 GTAC
Sau96I 72 GGCCC G^G[ACGT]CC
508 GGCCC
ScrFI 195 CCTGG CC^[ACGT]GG
459 CCTGG
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI AlwI BsaJI DdeI DpnII
EarI EcoT14I HaeIII HphI Ksp632I
MaeIII MboI MboII MnlI MseI
MslI NdeII NlaIII NlaIV Sau3AI
SfcI StyI Tru9I Tsp45I Tsp509I
TspRI