Restriction map for
pFN21AE6028
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HindIII HpaI
HpaII KasI KpnI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MspA1I MspI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI PvuII RsrII SacII SalI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 12 AAATTT [GA]^AATT[CT]
AflIII 836 ACACGT A^C[GA][CT]GT
AocI 876 CCTGAGG CC^T[ACGT]AGG
ApoI 12 AAATTT [GA]^AATT[CT]
AseI 205 ATTAAT AT^TAAT
AsnI 205 ATTAAT AT^TAAT
BanII 64 GAGCTC G[GA]GC[CT]^C
BbsI 894 GAAGAC GAAGAC(2/6)
BciVI 804 GGATAC GTATCC(6/5)
BpmI 67 CTCCAG CTGGAG(16/14)
BpuAI 894 GAAGAC GAAGAC(2/6)
BsaI 432 GGTCTC GGTCTC(1/5)
BsgI 778 GTGCAG GTGCAG(16/14)
BsmFI 757 GGGAC GGGAC(10/14)
Bsp1407I 392 TGTACA T^GTACA
BspHI 914 TCATGA T^CATGA
BsrGI 392 TGTACA T^GTACA
BstF5I 888 GGATG GGATG(2/0)
BstXI 293 CCACCCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 876 CCTGAGG CC^T[ACGT]AGG
Cac8I 342 GCTGGC GC[ACGT]^[ACGT]GC
DpnII 867 GATC ^GATC
DsaI 113 CCATGG C^C[GA][CT]GG
Eco81I 876 CCTGAGG CC^T[ACGT]AGG
EcoT14I 113 CCATGG C^C[AT][AT]GG
FokI 888 GGATG GGATG(9/13)
HaeIII 354 GGCC GG^CC
HincII 151 GTTGAC GT[CT]^[GA]AC
HindII 151 GTTGAC GT[CT]^[GA]AC
MaeII 838 ACGT A^CGT
MboI 867 GATC ^GATC
NcoI 113 CCATGG C^CATGG
NdeI 730 CATATG CA^TATG
NdeII 867 GATC ^GATC
PflMI 795 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 154 GACTC GAGTC(4/5)
PshBI 205 ATTAAT AT^TAAT
PstI 605 CTGCAG CTGCA^G
RcaI 914 TCATGA T^CATGA
SacI 64 GAGCTC GAGCT^C
SapI 506 GCTCTTC GCTCTTC(1/4)
Sau3AI 867 GATC ^GATC
SfaNI 131 GCATC GCATC(5/9)
SspBI 392 TGTACA T^GTACA
SspI 658 AATATT AAT^ATT
StyI 113 CCATGG C^C[AT][AT]GG
TaqI 159 TCGA T^CGA
TthHB8I 159 TCGA T^CGA
Van91I 795 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AciI 363 CCGC CCGC(-3/-1)
414 GCGG
AfaI 177 GTAC GT^AC
393 GTAC
AspHI 64 GAGCTC G[AT]GC[AT]^C
97 GTGCTC
BbvI 607 GCAGC GCAGC(8/12)
780 GCAGC
BfaI 53 CTAG C^TAG
643 CTAG
BmyI 64 GAGCTC G[AGT]GC[ACT]^C
97 GTGCTC
BsiHKAI 64 GAGCTC G[AT]GC[AT]^C
97 GTGCTC
Bsp1286I 64 GAGCTC G[AGT]GC[ACT]^C
97 GTGCTC
BsrDI 228 CATTGC GCAATG(2/0)
761 CATTGC
BsrI 300 ACTGG ACTGG(1/-1)
146 CCAGT
DdeI 29 CTGAG C^T[ACGT]AG
877 CTGAG
DraI 675 TTTAAA TTT^AAA
937 TTTAAA
EarI 79 CTCTTC CTCTTC(1/4)
507 CTCTTC
Ksp632I 79 CTCTTC CTCTTC(1/4)
507 CTCTTC
MaeI 53 CTAG C^TAG
643 CTAG
MslI 396 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
771 CATGTATGTG
MwoI 99 GCTCTATGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
346 GCTTTGATGGC
RsaI 177 GTAC GT^AC
393 GTAC
TseI 607 GCAGC G^C[AT]GC
780 GCAGC
Tsp45I 25 GTCAC ^GT[GC]AC
742 GTCAC
TspRI 27 CACTG CA[GC]TG[ACGT][ACGT]^
147 CAGTG
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI BsaJI BsiYI BslI BsmAI
BssKI BstNI EcoRII Fnu4HI HinfI
HphI ItaI MaeIII MboII MnlI
MseI MvaI NlaIII NlaIV ScrFI
SfcI TfiI Tru9I Tsp509I