Restriction map for
pFN21AE5944
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# NO CUT ENZYMES ( 171 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MroI
MseI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI TaqI Tru9I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 363 CCGC CCGC(-3/-1)
AlwI 207 GGATC GGATC(4/5)
AlwNI 424 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 232 GGACC G^G[AT]CC
BalI 371 TGGCCA TGG^CCA
BanI 72 GGCACC G^G[CT][GA]CC
BanII 722 GAGCCC G[GA]GC[CT]^C
BlpI 61 GCTGAGC GC^T[ACGT]AGC
BmyI 722 GAGCCC G[AGT]GC[ACT]^C
BpmI 156 CTCCAG CTGGAG(16/14)
Bpu1102I 61 GCTGAGC GC^T[ACGT]AGC
BsaAI 772 TACGTG [CT]AC^GT[GA]
BsaJI 833 CCCTGG C^C[ACGT][ACGT]GG
BsgI 775 GTGCAG GTGCAG(16/14)
BsiEI 360 CGACCG CG[GA][CT]^CG
BsmAI 542 GTCTC GTCTC(1/5)
Bsp1286I 722 GAGCCC G[AGT]GC[ACT]^C
BspHI 95 TCATGA T^CATGA
BspMI 653 ACCTGC ACCTGC(4/8)
BsrI 788 CCAGT ACTGG(1/-1)
BstYI 207 GGATCT [GA]^GATC[CT]
CelII 61 GCTGAGC GC^T[ACGT]AGC
Cfr13I 232 GGACC G^G[ACGT]CC
DraII 231 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 371 TGGCCA [CT]^GGCC[GA]
EcoO109I 231 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 55 GATATC GAT^ATC
HaeIII 372 GGCC GG^CC
MflI 207 GGATCT [GA]^GATC[CT]
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MslI 768 CATGTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 122 GCAATTTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NheI 300 GCTAGC G^CTAGC
PleI 154 GACTC GAGTC(4/5)
PpuMI 231 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 732 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 67 CTGCAG CTGCA^G
RcaI 95 TCATGA T^CATGA
Sau96I 232 GGACC G^G[ACGT]CC
SspI 925 AATATT AAT^ATT
XhoII 207 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 177 GTAC GT^AC
771 GTAC
Asp700I 27 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
31 GAATTCATTC
BfaI 301 CTAG C^TAG
472 CTAG
BsmFI 231 GGGAC GGGAC(10/14)
754 GGGAC
BssKI 158 CCAGG ^CC[ACGT]GG
834 CCTGG
BstF5I 660 CATCC GGATG(2/0)
680 GGATG
BstNI 158 CCAGG CC^[AT]GG
834 CCTGG
EarI 467 CTCTTC CTCTTC(1/4)
629 CTCTTC
EcoRI 31 GAATTC G^AATTC
684 GAATTC
EcoRII 158 CCAGG ^CC[AT]GG
834 CCTGG
FokI 660 CATCC GGATG(9/13)
680 GGATG
Ksp632I 467 CTCTTC CTCTTC(1/4)
629 CTCTTC
MaeI 301 CTAG C^TAG
472 CTAG
MaeII 515 ACGT A^CGT
773 ACGT
MboII 468 TCTTC GAAGA(8/7)
630 TCTTC
MvaI 158 CCAGG CC^[AT]GG
834 CCTGG
RsaI 177 GTAC GT^AC
771 GTAC
ScrFI 158 CCAGG CC^[ACGT]GG
834 CCTGG
SfcI 67 CTGCAG C^T[GA][CT]AG
864 CTATAG
TfiI 47 GAATC G^A[AT]TC
852 GAATC
XmnI 27 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
31 GAATTCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AcsI AluI ApoI BbvI BsiYI
BslI Cac8I DdeI DpnII Fnu4HI
HinfI HphI ItaI MaeIII MboI
MnlI NdeII NlaIII NlaIV Sau3AI
TseI Tsp45I Tsp509I TspRI