Restriction map for
pFN21AE5487
##################################
# NO CUT ENZYMES ( 188 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AfaI
AflII AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspHI AspI
AvaI AvaII AviII AvrII BalI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BcnI BfaI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaHI BsaJI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MaeI MamI MluI MluNI MroI
MscI MseI MspA1I MspI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StyI SwaI TaqI Tru9I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 808 ACATGT A^C[GA][CT]GT
AhdI 58 GACAAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 421 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 58 GACAAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 598 GGATCC G^GATCC
BbsI 163 GTCTTC GAAGAC(2/6)
BclI 450 TGATCA T^GATCA
BpmI 827 CTGGAG CTGGAG(16/14)
BpuAI 163 GTCTTC GAAGAC(2/6)
BsaI 870 GAGACC GGTCTC(1/5)
BseRI 635 CTCCTC GAGGAG(10/8)
BsmAI 870 GAGAC GTCTC(1/5)
BsmI 774 GCATTC GAATGC(1/-1)
BspLU11I 808 ACATGT A^CATGT
BspMI 35 ACCTGC ACCTGC(4/8)
BsrDI 439 GCAATG GCAATG(2/0)
BsrI 826 ACTGG ACTGG(1/-1)
BstYI 598 GGATCC [GA]^GATC[CT]
Eam1105I 58 GACAAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 685 CTCTTC CTCTTC(1/4)
EcoT22I 772 ATGCAT ATGCA^T
FbaI 450 TGATCA T^GATCA
HaeIII 798 GGCC GG^CC
Ksp632I 685 CTCTTC CTCTTC(1/4)
MaeII 536 ACGT A^CGT
MfeI 926 CAATTG C^AATTG
MflI 598 GGATCC [GA]^GATC[CT]
MunI 926 CAATTG C^AATTG
MwoI 436 GCTGCAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 772 ATGCAT ATGCA^T
PshAI 108 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SspI 915 AATATT AAT^ATT
StuI 797 AGGCCT AGG^CCT
XhoII 598 GGATCC [GA]^GATC[CT]
XmnI 421 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 599 GATCC GGATC(4/5)
598 GGATC
BbvI 156 GCTGC GCAGC(8/12)
436 GCTGC
BssKI 248 CCTGG ^CC[ACGT]GG
345 CCTGG
BstF5I 297 GGATG GGATG(2/0)
376 GGATG
BstNI 248 CCTGG CC^[AT]GG
345 CCTGG
Cac8I 301 GCTTGC GC[ACGT]^[ACGT]GC
541 GCTGGC
DdeI 277 CTTAG C^T[ACGT]AG
782 CTAAG
DpnII 451 GATC ^GATC
599 GATC
EcoRII 248 CCTGG ^CC[AT]GG
345 CCTGG
Fnu4HI 156 GCTGC GC^[ACGT]GC
436 GCTGC
FokI 297 GGATG GGATG(9/13)
376 GGATG
ItaI 156 GCTGC GC^[ACGT]GC
436 GCTGC
MboI 451 GATC ^GATC
599 GATC
MslI 903 CATCATAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
924 CACAATTGTG
MvaI 248 CCTGG CC^[AT]GG
345 CCTGG
NdeII 451 GATC ^GATC
599 GATC
NlaIV 251 GGCTCC GG[ACGT]^[ACGT]CC
598 GGATCC
PleI 4 GACTC GAGTC(4/5)
506 GAGTC
Sau3AI 451 GATC ^GATC
599 GATC
ScrFI 248 CCTGG CC^[ACGT]GG
345 CCTGG
TseI 156 GCTGC G^C[AT]GC
436 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AluI HinfI HphI MaeIII MboII
MnlI NlaIII NspI SfaNI SfcI
TfiI Tsp45I Tsp509I TspRI