Restriction map for
pFN21AE6015
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstYI BstZ17I CelII
Cfr10I Cfr13I ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FokI FseI FspI HaeII HapII
HgaI Hin1I HindIII HpaI HpaII
KasI KpnI KspI MamI MfeI
MflI MluI MroI MspA1I MspI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AfaI 177 GTAC GT^AC
AocI 873 CCTGAGG CC^T[ACGT]AGG
BalI 371 TGGCCA TGG^CCA
BanII 64 GAGCTC G[GA]GC[CT]^C
BbsI 88 GTCTTC GAAGAC(2/6)
BpmI 67 CTCCAG CTGGAG(16/14)
BpuAI 88 GTCTTC GAAGAC(2/6)
BspMI 718 ACCTGC ACCTGC(4/8)
BsrDI 228 CATTGC GCAATG(2/0)
BstXI 293 CCACCCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 873 CCTGAGG CC^T[ACGT]AGG
Cac8I 342 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 722 GCGC GCG^C
DpnII 864 GATC ^GATC
DraI 934 TTTAAA TTT^AAA
DsaI 113 CCATGG C^C[GA][CT]GG
EaeI 371 TGGCCA [CT]^GGCC[GA]
Eco81I 873 CCTGAGG CC^T[ACGT]AGG
EcoT14I 113 CCATGG C^C[AT][AT]GG
HhaI 722 GCGC GCG^C
HinP1I 722 GCGC G^CGC
HincII 151 GTTGAC GT[CT]^[GA]AC
HindII 151 GTTGAC GT[CT]^[GA]AC
MaeII 684 ACGT A^CGT
MboI 864 GATC ^GATC
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
NcoI 113 CCATGG C^CATGG
NdeI 730 CATATG CA^TATG
NdeII 864 GATC ^GATC
PflMI 795 CCACTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 154 GACTC GAGTC(4/5)
PstI 605 CTGCAG CTGCA^G
RsaI 177 GTAC GT^AC
SacI 64 GAGCTC GAGCT^C
SapI 506 GCTCTTC GCTCTTC(1/4)
Sau3AI 864 GATC ^GATC
SspI 658 AATATT AAT^ATT
StyI 113 CCATGG C^C[AT][AT]GG
TaqI 159 TCGA T^CGA
TthHB8I 159 TCGA T^CGA
Van91I 795 CCACTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 791 CCAACCACTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AciI 363 CCGC CCGC(-3/-1)
414 GCGG
AspHI 64 GAGCTC G[AT]GC[AT]^C
97 GTGCTC
BbvI 607 GCAGC GCAGC(8/12)
780 GCAGC
BciVI 680 GGATAC GTATCC(6/5)
804 GGATAC
BmyI 64 GAGCTC G[AGT]GC[ACT]^C
97 GTGCTC
BsiHKAI 64 GAGCTC G[AT]GC[AT]^C
97 GTGCTC
BsmFI 757 GGGAC GGGAC(10/14)
435 GTCCC
Bsp1286I 64 GAGCTC G[AGT]GC[ACT]^C
97 GTGCTC
EarI 79 CTCTTC CTCTTC(1/4)
507 CTCTTC
HaeIII 354 GGCC GG^CC
372 GGCC
Ksp632I 79 CTCTTC CTCTTC(1/4)
507 CTCTTC
MseI 901 TTAA T^TAA
935 TTAA
MslI 396 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
834 CACATTGGTG
MwoI 99 GCTCTATGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
346 GCTGTGATGGC
NlaIV 3 GGCTCC GG[ACGT]^[ACGT]CC
122 GGAACC
SfaNI 339 GATGC GCATC(5/9)
131 GCATC
Tru9I 901 TTAA T^TAA
935 TTAA
TseI 607 GCAGC G^C[AT]GC
780 GCAGC
Tsp45I 25 GTCAC ^GT[GC]AC
742 GTCAC
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AcsI AluI ApoI BfaI BsaJI
BsiYI BslI BsmAI BsrI BssKI
BstNI DdeI EcoRII Fnu4HI HinfI
HphI ItaI MaeI MaeIII MboII
MnlI MvaI NlaIII ScrFI SfcI
TfiI Tsp509I TspRI