Restriction map for
pFN21AE5945
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# NO CUT ENZYMES ( 192 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI ApoI AscI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI KspI
MaeI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MspA1I
MspI MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 22 enzymes) #
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AciI 150 CCGC CCGC(-3/-1)
AfaI 323 GTAC GT^AC
AlwNI 56 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 682 ATTAAT AT^TAAT
AsnI 682 ATTAAT AT^TAAT
Asp700I 705 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BsaJI 800 CCTTGG C^C[ACGT][ACGT]GG
BsgI 564 CTGCAC GTGCAG(16/14)
BsmAI 724 GTCTC GTCTC(1/5)
BsmBI 723 CGTCTC CGTCTC(1/5)
BsrI 436 ACTGG ACTGG(1/-1)
BstXI 614 CCACCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 463 GACTCCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 800 CCTTGG C^C[AT][AT]GG
HgaI 722 GCGTC GACGC(5/10)
HindIII 707 AAGCTT A^AGCTT
PshBI 682 ATTAAT AT^TAAT
PstI 378 CTGCAG CTGCA^G
RsaI 323 GTAC GT^AC
SfcI 378 CTGCAG C^T[GA][CT]AG
StyI 800 CCTTGG C^C[AT][AT]GG
XmnI 705 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AvaII 69 GGTCC G^G[AT]CC
156 GGACC
BbsI 920 GAAGAC GAAGAC(2/6)
313 GTCTTC
BbvI 370 GCAGC GCAGC(8/12)
287 GCTGC
BpmI 160 CTCCAG CTGGAG(16/14)
930 CTCCAG
BpuAI 920 GAAGAC GAAGAC(2/6)
313 GTCTTC
BseRI 97 CTCCTC GAGGAG(10/8)
345 CTCCTC
BsmFI 48 GGGAC GGGAC(10/14)
627 GTCCC
BssKI 153 CCTGG ^CC[ACGT]GG
932 CCAGG
BstNI 153 CCTGG CC^[AT]GG
932 CCAGG
EarI 64 GAAGAG CTCTTC(1/4)
806 GAAGAG
EcoRII 153 CCTGG ^CC[AT]GG
932 CCAGG
Fnu4HI 287 GCTGC GC^[ACGT]GC
370 GCAGC
HaeIII 354 GGCC GG^CC
440 GGCC
HphI 195 TCACC GGTGA(8/7)
743 TCACC
ItaI 287 GCTGC GC^[ACGT]GC
370 GCAGC
Ksp632I 64 GAAGAG CTCTTC(1/4)
806 GAAGAG
MseI 683 TTAA T^TAA
778 TTAA
MvaI 153 CCTGG CC^[AT]GG
932 CCAGG
NlaIV 610 GGTTCC GG[ACGT]^[ACGT]CC
819 GGCTCC
ScrFI 153 CCTGG CC^[ACGT]GG
932 CCAGG
SfaNI 135 GATGC GCATC(5/9)
646 GCATC
TfiI 843 GATTC G^A[AT]TC
855 GAATC
Tru9I 683 TTAA T^TAA
778 TTAA
TseI 287 GCTGC G^C[AT]GC
370 GCAGC
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AluI BstF5I Cfr13I DdeI DpnII
FokI HinfI MaeIII MboI MboII
MnlI MslI NdeII NlaIII PleI
Sau3AI Sau96I Tsp45I Tsp509I TspRI