Restriction map for
pFN21AE5946
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# NO CUT ENZYMES ( 185 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraII DraIII DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HpaI HpaII KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AciI 150 CCGC CCGC(-3/-1)
AfaI 323 GTAC GT^AC
AlwI 69 GATCC GGATC(4/5)
AseI 682 ATTAAT AT^TAAT
AsnI 682 ATTAAT AT^TAAT
Asp700I 705 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 156 GGACC G^G[AT]CC
BbsI 920 GAAGAC GAAGAC(2/6)
BpuAI 920 GAAGAC GAAGAC(2/6)
BsaJI 800 CCTTGG C^C[ACGT][ACGT]GG
BseRI 345 CTCCTC GAGGAG(10/8)
BsgI 564 CTGCAC GTGCAG(16/14)
BsmAI 724 GTCTC GTCTC(1/5)
BsmBI 723 CGTCTC CGTCTC(1/5)
BsrI 436 ACTGG ACTGG(1/-1)
BstXI 614 CCACCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 68 AGATCC [GA]^GATC[CT]
DraI 777 TTTAAA TTT^AAA
DrdI 463 GACTCCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 800 CCTTGG C^C[AT][AT]GG
HgaI 722 GCGTC GACGC(5/10)
HincII 472 GTCAAC GT[CT]^[GA]AC
HindII 472 GTCAAC GT[CT]^[GA]AC
HindIII 707 AAGCTT A^AGCTT
MflI 68 AGATCC [GA]^GATC[CT]
NlaIV 610 GGTTCC GG[ACGT]^[ACGT]CC
PshBI 682 ATTAAT AT^TAAT
PstI 378 CTGCAG CTGCA^G
RsaI 323 GTAC GT^AC
SfaNI 646 GCATC GCATC(5/9)
SfcI 378 CTGCAG C^T[GA][CT]AG
StyI 800 CCTTGG C^C[AT][AT]GG
XhoII 68 AGATCC [GA]^GATC[CT]
XmnI 705 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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BbvI 370 GCAGC GCAGC(8/12)
287 GCTGC
BpmI 160 CTCCAG CTGGAG(16/14)
930 CTCCAG
BsmFI 48 GGGAC GGGAC(10/14)
627 GTCCC
BssKI 153 CCTGG ^CC[ACGT]GG
932 CCAGG
BstNI 153 CCTGG CC^[AT]GG
932 CCAGG
Cfr13I 156 GGACC G^G[ACGT]CC
439 GGGCC
EarI 64 GAAGAG CTCTTC(1/4)
806 GAAGAG
EcoRII 153 CCTGG ^CC[AT]GG
932 CCAGG
Fnu4HI 287 GCTGC GC^[ACGT]GC
370 GCAGC
HaeIII 354 GGCC GG^CC
440 GGCC
ItaI 287 GCTGC GC^[ACGT]GC
370 GCAGC
Ksp632I 64 GAAGAG CTCTTC(1/4)
806 GAAGAG
MaeII 476 ACGT A^CGT
515 ACGT
MseI 683 TTAA T^TAA
778 TTAA
MvaI 153 CCTGG CC^[AT]GG
932 CCAGG
Sau96I 156 GGACC G^G[ACGT]CC
439 GGGCC
ScrFI 153 CCTGG CC^[ACGT]GG
932 CCAGG
TfiI 843 GATTC G^A[AT]TC
855 GAATC
Tru9I 683 TTAA T^TAA
778 TTAA
TseI 287 GCTGC G^C[AT]GC
370 GCAGC
TspRI 435 CACTG CA[GC]TG[ACGT][ACGT]^
567 CACTG
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI BstF5I DdeI DpnII FokI
HinfI HphI MaeIII MboI MboII
MnlI MslI NdeII NlaIII PleI
Sau3AI Tsp45I Tsp509I