Restriction map for
pFN21AE5920
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AvaII AvrII BalI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I CelII
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoRI EcoT14I EcoT22I
FauI FseI HaeII HapII HgaI
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI TaqI Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AcsI 249 GAATTT [GA]^AATT[CT]
ApoI 249 GAATTT [GA]^AATT[CT]
AviII 718 TGCGCA TGC^GCA
BamHI 677 GGATCC G^GATCC
BbsI 31 GTCTTC GAAGAC(2/6)
BclI 134 TGATCA T^GATCA
BpuAI 31 GTCTTC GAAGAC(2/6)
BsaI 755 GGTCTC GGTCTC(1/5)
BseRI 219 CTCCTC GAGGAG(10/8)
BsmAI 756 GTCTC GTCTC(1/5)
BstEII 24 GGTTACC G^GT[ACGT]ACC
BstPI 24 GGTTACC G^GT[ACGT]ACC
BstXI 422 CCAACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoO65I 24 GGTTACC G^GT[ACGT]ACC
EcoRV 471 GATATC GAT^ATC
FbaI 134 TGATCA T^GATCA
FspI 718 TGCGCA TGC^GCA
MaeII 139 ACGT A^CGT
MseI 142 TTAA T^TAA
NdeI 443 CATATG CA^TATG
NlaIV 677 GGATCC GG[ACGT]^[ACGT]CC
PflMI 726 CCACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 905 GAGTC GAGTC(4/5)
SapI 895 GCTCTTC GCTCTTC(1/4)
SfcI 864 CTACAG C^T[GA][CT]AG
StuI 704 AGGCCT AGG^CCT
Tru9I 142 TTAA T^TAA
Van91I 726 CCACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AfaI 177 GTAC GT^AC
303 GTAC
AocI 235 CCTAAGG CC^T[ACGT]AGG
672 CCTGAGG
Asp700I 149 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
702 GAAGGCCTTC
BbvI 688 GCAGC GCAGC(8/12)
287 GCTGC
BsaJI 126 CCTGGG C^C[ACGT][ACGT]GG
803 CCCAGG
BsmFI 129 GGGAC GGGAC(10/14)
408 GTCCC
BspMI 715 ACCTGC ACCTGC(4/8)
810 GCAGGT
BsrI 425 ACTGG ACTGG(1/-1)
681 CCAGT
BssKI 126 CCTGG ^CC[ACGT]GG
804 CCAGG
BstNI 126 CCTGG CC^[AT]GG
804 CCAGG
BstYI 268 AGATCC [GA]^GATC[CT]
677 GGATCC
Bsu36I 235 CCTAAGG CC^T[ACGT]AGG
672 CCTGAGG
CfoI 719 GCGC GCG^C
808 GCGC
Eco81I 235 CCTAAGG CC^T[ACGT]AGG
672 CCTGAGG
EcoRII 126 CCTGG ^CC[AT]GG
804 CCAGG
Fnu4HI 287 GCTGC GC^[ACGT]GC
688 GCAGC
HaeIII 354 GGCC GG^CC
705 GGCC
HhaI 719 GCGC GCG^C
808 GCGC
HinP1I 719 GCGC G^CGC
808 GCGC
ItaI 287 GCTGC GC^[ACGT]GC
688 GCAGC
MflI 268 AGATCC [GA]^GATC[CT]
677 GGATCC
MvaI 126 CCTGG CC^[AT]GG
804 CCAGG
RsaI 177 GTAC GT^AC
303 GTAC
ScrFI 126 CCTGG CC^[ACGT]GG
804 CCAGG
TseI 287 GCTGC G^C[AT]GC
688 GCAGC
Tsp45I 533 GTGAC ^GT[GC]AC
643 GTCAC
TspRI 18 CACTG CA[GC]TG[ACGT][ACGT]^
111 CACTG
XhoII 268 AGATCC [GA]^GATC[CT]
677 GGATCC
XmnI 149 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
702 GAAGGCCTTC
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AluI AlwI BsiYI BslI BstF5I
Cac8I DdeI DpnII EarI FokI
HinfI HphI Ksp632I MaeIII MboI
MboII MnlI MslI MwoI NdeII
NlaIII Sau3AI SfaNI TfiI Tsp509I