Restriction map for
pFN21AE6081
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI AlwI ApaI AscI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoT14I EcoT22I FauI
FbaI FseI FspI HaeIII HapII
HgaI Hin1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
StuI StyI TaqI Tth111I TthHB8I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 167 AAATTC [GA]^AATT[CT]
Alw44I 290 GTGCAC G^TGCAC
AlwNI 586 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 689 CCTCAGG CC^T[ACGT]AGG
Aor51HI 759 AGCGCT AGC^GCT
ApaLI 290 GTGCAC G^TGCAC
ApoI 167 AAATTC [GA]^AATT[CT]
AseI 151 ATTAAT AT^TAAT
AsnI 151 ATTAAT AT^TAAT
AspHI 290 GTGCAC G[AT]GC[AT]^C
AvaII 471 GGTCC G^G[AT]CC
BmyI 290 GTGCAC G[AGT]GC[ACT]^C
BsgI 606 GTGCAG GTGCAG(16/14)
BsiHKAI 290 GTGCAC G[AT]GC[AT]^C
BsiYI 336 CCTTATCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 336 CCTTATCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 290 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 565 TGTACA T^GTACA
BsrDI 349 GCAATG GCAATG(2/0)
BsrGI 565 TGTACA T^GTACA
BsrI 468 ACTGG ACTGG(1/-1)
Bsu36I 689 CCTCAGG CC^T[ACGT]AGG
Cac8I 243 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 760 GCGC GCG^C
Cfr13I 471 GGTCC G^G[ACGT]CC
DpnII 579 GATC ^GATC
DraI 945 TTTAAA TTT^AAA
EarI 548 CTCTTC CTCTTC(1/4)
Eco47III 759 AGCGCT AGC^GCT
Eco81I 689 CCTCAGG CC^T[ACGT]AGG
EcoRV 538 GATATC GAT^ATC
HaeII 759 AGCGCT [GA]GCGC^[CT]
HhaI 760 GCGC GCG^C
HinP1I 760 GCGC G^CGC
HphI 743 TCACC GGTGA(8/7)
Ksp632I 548 CTCTTC CTCTTC(1/4)
MboI 579 GATC ^GATC
MspA1I 213 CAGCTG C[AC]G^C[GT]G
NdeII 579 GATC ^GATC
NlaIV 508 GGCTCC GG[ACGT]^[ACGT]CC
PshBI 151 ATTAAT AT^TAAT
PvuII 213 CAGCTG CAG^CTG
Sau3AI 579 GATC ^GATC
Sau96I 471 GGTCC G^G[ACGT]CC
SfcI 221 CTACAG C^T[GA][CT]AG
SspBI 565 TGTACA T^GTACA
SwaI 944 ATTTAAAT ATTT^AAAT
TfiI 47 GATTC G^A[AT]TC
XcmI 623 CCAGCACTTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 12 enzymes) #
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BssKI 263 CCAGG ^CC[ACGT]GG
342 CCTGG
BstNI 263 CCAGG CC^[AT]GG
342 CCTGG
EcoRII 263 CCAGG ^CC[AT]GG
342 CCTGG
MaeIII 73 GTCAC ^GT[ACGT]AC
742 GTCAC
MboII 308 TCTTC GAAGA(8/7)
549 TCTTC
MslI 627 CACTTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
666 CATCACTGTG
MvaI 263 CCAGG CC^[AT]GG
342 CCTGG
MwoI 313 GCTTCTTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
346 GCAGCAATGGC
PleI 71 GAGTC GAGTC(4/5)
416 GAGTC
ScrFI 263 CCAGG CC^[ACGT]GG
342 CCTGG
SspI 148 AATATT AAT^ATT
639 AATATT
Tsp45I 73 GTCAC ^GT[GC]AC
742 GTCAC
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
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AfaI AluI BbvI BfaI BstF5I
DdeI Fnu4HI FokI HinfI ItaI
MaeI MnlI MseI NlaIII RsaI
SfaNI Tru9I TseI Tsp509I TspRI