Restriction map for
pFN21AE5442
##################################
# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaBI BsaHI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I Bsp1407I BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I CelII
CfoI CpoI DraI DraIII DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT14I FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NlaIV NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PmeI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 418 GTCTAC GT^[AC][GT]AC
AflIII 718 ACGTGT A^C[GA][CT]GT
AgeI 362 ACCGGT A^CCGGT
AocI 873 CCTCAGG CC^T[ACGT]AGG
AvaI 127 CTCGGG C^[CT]CG[GA]G
BbrPI 717 CACGTG CAC^GTG
BclI 401 TGATCA T^GATCA
BcnI 437 CCGGG CC^[GC]GG
BfaI 785 CTAG C^TAG
BpmI 61 CTGGAG CTGGAG(16/14)
BsaAI 717 CACGTG [CT]AC^GT[GA]
BsaI 780 GAGACC GGTCTC(1/5)
BsaWI 362 ACCGGT [AT]^CCGG[AT]
BseRI 645 CTCCTC GAGGAG(10/8)
BsmAI 780 GAGAC GTCTC(1/5)
BsoBI 127 CTCGGG C^[CT]CG[GA]G
BspDI 151 ATCGAT AT^CGAT
BspMI 760 ACCTGC ACCTGC(4/8)
BsrFI 362 ACCGGT [GA]^CCGG[CT]
BsrI 117 ACTGG ACTGG(1/-1)
Bsu36I 873 CCTCAGG CC^T[ACGT]AGG
Cfr10I 362 ACCGGT [GA]^CCGG[CT]
ClaI 151 ATCGAT AT^CGAT
DraII 925 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 508 GACTCCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 371 CGGCCA [CT]^GGCC[GA]
Eco81I 873 CCTCAGG CC^T[ACGT]AGG
EcoNI 801 CCTGAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 925 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 384 CCCGC CCCGC(4/6)
FbaI 401 TGATCA T^GATCA
HincII 472 GTCAAC GT[CT]^[GA]AC
HindII 472 GTCAAC GT[CT]^[GA]AC
MaeI 785 CTAG C^TAG
MspA1I 385 CCGCTG C[AC]G^C[GT]G
NciI 437 CCGGG CC^[GC]GG
NdeI 375 CATATG CA^TATG
PinAI 362 ACCGGT A^CCGGT
PmaCI 717 CACGTG CAC^GTG
PmlI 717 CACGTG CAC^GTG
PpuMI 925 AGGACCT [GA]G^G[AT]CC[CT]
PstI 449 CTGCAG CTGCA^G
ScaI 114 AGTACT AGT^ACT
SspI 616 AATATT AAT^ATT
TaqI 152 TCGA T^CGA
TthHB8I 152 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 385 CCGC CCGC(-3/-1)
370 GCGG
AfaI 115 GTAC GT^AC
303 GTAC
AlwI 135 GATCC GGATC(4/5)
149 GGATC
AvaII 413 GGACC G^G[AT]CC
926 GGACC
BsaJI 317 CCCTGG C^C[ACGT][ACGT]GG
437 CCGGGG
BsmFI 625 GGGAC GGGAC(10/14)
542 GTCCC
BsmI 331 GAATGC GAATGC(1/-1)
684 GCATTC
Cfr13I 413 GGACC G^G[ACGT]CC
926 GGACC
EarI 664 CTCTTC CTCTTC(1/4)
930 CTCTTC
EcoT22I 333 ATGCAT ATGCA^T
682 ATGCAT
HaeIII 354 GGCC GG^CC
372 GGCC
HapII 363 CCGG C^CGG
437 CCGG
HpaII 363 CCGG C^CGG
437 CCGG
Ksp632I 664 CTCTTC CTCTTC(1/4)
930 CTCTTC
MseI 142 TTAA T^TAA
349 TTAA
MslI 168 CACACCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
834 CACAGTGGTG
MspI 363 CCGG C^CGG
437 CCGG
NsiI 333 ATGCAT ATGCA^T
682 ATGCAT
PleI 508 GACTC GAGTC(4/5)
627 GACTC
RsaI 115 GTAC GT^AC
303 GTAC
Sau96I 413 GGACC G^G[ACGT]CC
926 GGACC
Tru9I 142 TTAA T^TAA
349 TTAA
Tsp45I 506 GTGAC ^GT[GC]AC
536 GTGAC
TspRI 23 CACTG CA[GC]TG[ACGT][ACGT]^
836 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI BbvI BsiYI BslI BssKI
BstF5I BstNI Cac8I DdeI DpnII
EcoRII Fnu4HI FokI HinfI HphI
ItaI MaeII MaeIII MboI MboII
MnlI MvaI MwoI NdeII NlaIII
Sau3AI ScrFI SfaNI SfcI TfiI
TseI Tsp509I