Restriction map for
pFN21AE5910
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI Aor51HI ApaLI AscI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoT14I EcoT22I
FauI FbaI Fnu4HI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI ItaI KasI KpnI
Ksp632I KspI MaeII MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TseI Tth111I
TthHB8I Van91I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 335 GTCTAC GT^[AC][GT]AC
AcsI 920 AAATTT [GA]^AATT[CT]
AflIII 404 ACATGT A^C[GA][CT]GT
AluI 708 AGCT AG^CT
AlwNI 323 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 873 CCTGAGG CC^T[ACGT]AGG
ApaI 232 GGGCCC GGGCC^C
ApoI 920 AAATTT [GA]^AATT[CT]
AspHI 290 GTGCTC G[AT]GC[AT]^C
BanII 232 GGGCCC G[GA]GC[CT]^C
BbsI 307 GTCTTC GAAGAC(2/6)
BbvCI 691 GCTGAGG CCTCAGC(-5/-2)
BglII 249 AGATCT A^GATCT
BpuAI 307 GTCTTC GAAGAC(2/6)
BsaJI 753 CCAGGG C^C[ACGT][ACGT]GG
BsiHKAI 290 GTGCTC G[AT]GC[AT]^C
BspLU11I 404 ACATGT A^CATGT
BssKI 753 CCAGG ^CC[ACGT]GG
BstAPI 413 GCAGAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 753 CCAGG CC^[AT]GG
BstXI 236 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 249 AGATCT [GA]^GATC[CT]
Bsu36I 873 CCTGAGG CC^T[ACGT]AGG
DraII 896 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 781 CGGCCA [CT]^GGCC[GA]
Eco81I 873 CCTGAGG CC^T[ACGT]AGG
EcoO109I 896 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 753 CCAGG ^CC[AT]GG
EcoRV 538 GATATC GAT^ATC
HapII 780 CCGG C^CGG
HincII 400 GTCAAC GT[CT]^[GA]AC
HindII 400 GTCAAC GT[CT]^[GA]AC
HinfI 325 GATTC G^A[ACGT]TC
HpaII 780 CCGG C^CGG
HphI 599 TCACC GGTGA(8/7)
MaeIII 73 GTGAC ^GT[ACGT]AC
MflI 249 AGATCT [GA]^GATC[CT]
MspI 780 CCGG C^CGG
MvaI 753 CCAGG CC^[AT]GG
NdeI 475 CATATG CA^TATG
ScrFI 753 CCAGG CC^[ACGT]GG
TfiI 325 GATTC G^A[AT]TC
Tsp45I 73 GTGAC ^GT[GC]AC
XbaI 801 TCTAGA T^CTAGA
XhoII 249 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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AciI 492 CCGC CCGC(-3/-1)
739 GCGG
AfaI 366 GTAC GT^AC
626 GTAC
AseI 109 ATTAAT AT^TAAT
520 ATTAAT
AsnI 109 ATTAAT AT^TAAT
520 ATTAAT
BfaI 410 CTAG C^TAG
802 CTAG
BmyI 232 GGGCCC G[AGT]GC[ACT]^C
290 GTGCTC
BseRI 18 CTCCTC GAGGAG(10/8)
545 CTCCTC
BsiYI 277 CCCTTCTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
356 CCTTTGATCGG
BslI 277 CCCTTCTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
356 CCTTTGATCGG
Bsp1286I 232 GGGCCC G[AGT]GC[ACT]^C
290 GTGCTC
BstF5I 663 CATCC GGATG(2/0)
885 GGATG
FokI 663 CATCC GGATG(9/13)
885 GGATG
MaeI 410 CTAG C^TAG
802 CTAG
MslI 168 CACACCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
834 CACCCTTGTG
MwoI 234 GCCCAAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
413 GCAGAGTGTGC
NlaIV 232 GGGCCC GG[ACGT]^[ACGT]CC
843 GGTTCC
NspI 404 ACATGT [GA]CATG^[CT]
718 ACATGC
PshBI 109 ATTAAT AT^TAAT
520 ATTAAT
RsaI 366 GTAC GT^AC
626 GTAC
SfaNI 240 GATGC GCATC(5/9)
463 GATGC
TspRI 347 CAGTG CA[GC]TG[ACGT][ACGT]^
482 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
BsrI Cfr13I DdeI DpnII HaeIII
MboI MboII MnlI MseI NdeII
NlaIII Sau3AI Sau96I Tru9I Tsp509I