Restriction map for
pFN21AE5983
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BanII BbrPI BcgI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraII DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HapII
HgaI HindIII HpaI HpaII KpnI
KspI MfeI MluI MroI MspA1I
MspI MunI MvnI NaeI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcyI 701 GGCGCC G[GA]^CG[CT]C
AflIII 407 ACATGT A^C[GA][CT]GT
AluI 348 AGCT AG^CT
AlwNI 589 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 272 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 483 GTGCTC G[AT]GC[AT]^C
AvaII 29 GGACC G^G[AT]CC
BamHI 442 GGATCC G^GATCC
BbeI 701 GGCGCC GGCGC^C
BbsI 928 GTCTTC GAAGAC(2/6)
BbvCI 189 CCTCAGC CCTCAGC(-5/-2)
BciVI 897 GGATAC GTATCC(6/5)
BclI 143 TGATCA T^GATCA
BfaI 121 CTAG C^TAG
BpmI 801 CTCCAG CTGGAG(16/14)
BpuAI 928 GTCTTC GAAGAC(2/6)
BsaBI 138 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 701 GGCGCC G[GA]^CG[CT]C
BseRI 546 CTCCTC GAGGAG(10/8)
BsiHKAI 483 GTGCTC G[AT]GC[AT]^C
BsmAI 460 GTCTC GTCTC(1/5)
BspLU11I 407 ACATGT A^CATGT
BsrDI 231 CATTGC GCAATG(2/0)
BstXI 239 CCAAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 442 GGATCC [GA]^GATC[CT]
Cac8I 425 GCGTGC GC[ACGT]^[ACGT]GC
Cfr13I 29 GGACC G^G[ACGT]CC
DraI 492 TTTAAA TTT^AAA
DrdI 919 GACACCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 143 TGATCA T^GATCA
HaeII 701 GGCGCC [GA]GCGC^[CT]
Hin1I 701 GGCGCC G[GA]^CG[CT]C
HincII 403 GTTGAC GT[CT]^[GA]AC
HindII 403 GTTGAC GT[CT]^[GA]AC
KasI 701 GGCGCC G^GCGCC
MaeI 121 CTAG C^TAG
MamI 138 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 442 GGATCC [GA]^GATC[CT]
MslI 837 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 349 GCTGTGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 701 GGCGCC GG^CGCC
NcoI 756 CCATGG C^CATGG
NspI 407 ACATGT [GA]CATG^[CT]
PleI 458 GAGTC GAGTC(4/5)
Sau96I 29 GGACC G^G[ACGT]CC
SfcI 870 CTACAG C^T[GA][CT]AG
SfoI 701 GGCGCC GGC^GCC
SmlI 679 CTCAAG C^T[CT][GA]AG
SspI 127 AATATT AAT^ATT
Tsp509I 302 AATT ^AATT
XhoII 442 GGATCC [GA]^GATC[CT]
XmnI 272 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AciI 366 CCGC CCGC(-3/-1)
705 CCGC
BalI 98 TGGCCA TGG^CCA
374 TGGCCA
BanI 701 GGCGCC G^G[CT][GA]CC
760 GGCACC
BmyI 483 GTGCTC G[AGT]GC[ACT]^C
784 GTGCCC
BsiYI 366 CCGCTTCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
446 CCTATGCCTGG
BslI 366 CCGCTTCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
446 CCTATGCCTGG
Bsp1286I 483 GTGCTC G[AGT]GC[ACT]^C
784 GTGCCC
BsrI 915 ACTGG ACTGG(1/-1)
588 CCAGT
CfoI 702 GCGC GCG^C
807 GCGC
DsaI 756 CCATGG C^C[GA][CT]GG
839 CCGTGG
EaeI 98 TGGCCA [CT]^GGCC[GA]
374 TGGCCA
EarI 82 CTCTTC CTCTTC(1/4)
769 CTCTTC
EcoT14I 470 CCTTGG C^C[AT][AT]GG
756 CCATGG
HhaI 702 GCGC GCG^C
807 GCGC
HinP1I 702 GCGC G^CGC
807 GCGC
Ksp632I 82 CTCTTC CTCTTC(1/4)
769 CTCTTC
MaeII 34 ACGT A^CGT
481 ACGT
MluNI 98 TGGCCA TGG^CCA
374 TGGCCA
MscI 98 TGGCCA TGG^CCA
374 TGGCCA
MseI 493 TTAA T^TAA
617 TTAA
SfaNI 290 GATGC GCATC(5/9)
684 GATGC
StyI 470 CCTTGG C^C[AT][AT]GG
756 CCATGG
TfiI 334 GAATC G^A[AT]TC
858 GAATC
Tru9I 493 TTAA T^TAA
617 TTAA
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AfaI AlwI BbvI BsaJI BsmFI
BssKI BstF5I BstNI DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MboI MboII
MnlI MvaI NdeII NlaIII NlaIV
RsaI Sau3AI ScrFI TseI Tsp45I
TspRI