Restriction map for
pFN21AE5465
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmFI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HphI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MroI MspA1I MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AciI 707 CCGC CCGC(-3/-1)
AcsI 25 GAATTC [GA]^AATT[CT]
AfaI 171 GTAC GT^AC
AflII 483 CTTAAG C^TTAAG
AluI 465 AGCT AG^CT
ApoI 25 GAATTC [GA]^AATT[CT]
AseI 234 ATTAAT AT^TAAT
AsnI 234 ATTAAT AT^TAAT
AvaII 502 GGACC G^G[AT]CC
BalI 365 TGGCCA TGG^CCA
BamHI 51 GGATCC G^GATCC
BfrI 483 CTTAAG C^TTAAG
BglII 326 AGATCT A^GATCT
BsaJI 791 CCATGG C^C[ACGT][ACGT]GG
BsmAI 717 GTCTC GTCTC(1/5)
BsmBI 716 CGTCTC CGTCTC(1/5)
DrdI 135 GACCATCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 791 CCATGG C^C[GA][CT]GG
EaeI 365 TGGCCA [CT]^GGCC[GA]
EcoRI 25 GAATTC G^AATTC
EcoT14I 791 CCATGG C^C[AT][AT]GG
HapII 150 CCGG C^CGG
HinfI 202 GATTC G^A[ACGT]TC
HpaII 150 CCGG C^CGG
MaeII 715 ACGT A^CGT
MluNI 365 TGGCCA TGG^CCA
MscI 365 TGGCCA TGG^CCA
MspI 150 CCGG C^CGG
MwoI 700 GCTCTCTCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 791 CCATGG C^CATGG
PshAI 288 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 234 ATTAAT AT^TAAT
RsaI 171 GTAC GT^AC
SfcI 461 CTACAG C^T[GA][CT]AG
StyI 791 CCATGG C^C[AT][AT]GG
TfiI 202 GATTC G^A[AT]TC
Tsp45I 133 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AlwI 52 GATCC GGATC(4/5)
51 GGATC
BclI 516 TGATCA T^GATCA
575 TGATCA
BfaI 157 CTAG C^TAG
230 CTAG
BsmI 864 GAATGC GAATGC(1/-1)
660 GCATTC
BspHI 398 TCATGA T^CATGA
626 TCATGA
BspMI 581 ACCTGC ACCTGC(4/8)
408 GCAGGT
BssKI 437 CCTGG ^CC[ACGT]GG
477 CCTGG
BstF5I 420 CATCC GGATG(2/0)
243 GGATG
BstNI 437 CCTGG CC^[AT]GG
477 CCTGG
BstYI 51 GGATCC [GA]^GATC[CT]
326 AGATCT
Cac8I 404 GCCAGC GC[ACGT]^[ACGT]GC
709 GCTTGC
Cfr13I 502 GGACC G^G[ACGT]CC
748 GGCCC
EcoRII 437 CCTGG ^CC[AT]GG
477 CCTGG
FbaI 516 TGATCA T^GATCA
575 TGATCA
FokI 420 CATCC GGATG(9/13)
243 GGATG
MaeI 157 CTAG C^TAG
230 CTAG
MaeIII 133 GTGAC ^GT[ACGT]AC
386 GTTAC
MboII 690 GAAGA GAAGA(8/7)
83 TCTTC
MflI 51 GGATCC [GA]^GATC[CT]
326 AGATCT
MvaI 437 CCTGG CC^[AT]GG
477 CCTGG
NlaIV 51 GGATCC GG[ACGT]^[ACGT]CC
502 GGACCC
RcaI 398 TCATGA T^CATGA
626 TCATGA
Sau96I 502 GGACC G^G[ACGT]CC
748 GGCCC
ScrFI 437 CCTGG CC^[ACGT]GG
477 CCTGG
SfaNI 244 GATGC GCATC(5/9)
416 GCATC
XcmI 782 CCACTTTCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
791 CCATGGACAAGATGG
XhoII 51 GGATCC [GA]^GATC[CT]
326 AGATCT
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
BbvI BsiYI BslI DdeI DpnII
Fnu4HI HaeIII ItaI MboI MnlI
MseI MslI NdeII NlaIII Sau3AI
SmlI Tru9I TseI Tsp509I TspRI