Restriction map for
pFN21AE5475
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmFI BsmI BsoBI BspDI BspEI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MaeII MamI MfeI MluI
MroI MspI MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 488 GTCTAC GT^[AC][GT]AC
AcsI 25 GAATTT [GA]^AATT[CT]
AflIII 533 ACATGT A^C[GA][CT]GT
Alw44I 454 GTGCAC G^TGCAC
AlwI 52 GATCC GGATC(4/5)
ApaLI 454 GTGCAC G^TGCAC
ApoI 25 GAATTT [GA]^AATT[CT]
AseI 801 ATTAAT AT^TAAT
AsnI 801 ATTAAT AT^TAAT
AspHI 454 GTGCAC G[AT]GC[AT]^C
BalI 434 TGGCCA TGG^CCA
BanII 159 GGGCTC G[GA]GC[CT]^C
BbsI 328 GTCTTC GAAGAC(2/6)
BcgI 406 CGACTGGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 959 CTAG C^TAG
BpuAI 328 GTCTTC GAAGAC(2/6)
BsaI 3 GAGACC GGTCTC(1/5)
BseRI 121 CTCCTC GAGGAG(10/8)
BsiHKAI 454 GTGCAC G[AT]GC[AT]^C
Bsp1407I 482 TGTACA T^GTACA
BspHI 398 TCATGA T^CATGA
BspLU11I 533 ACATGT A^CATGT
BspMI 709 ACCTGC ACCTGC(4/8)
BsrDI 14 GCAATG GCAATG(2/0)
BsrGI 482 TGTACA T^GTACA
BsrI 408 ACTGG ACTGG(1/-1)
BssKI 36 CCTGG ^CC[ACGT]GG
BstNI 36 CCTGG CC^[AT]GG
BstYI 51 AGATCC [GA]^GATC[CT]
Cac8I 280 GCTTGC GC[ACGT]^[ACGT]GC
DraIII 457 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 517 GACCACTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 434 TGGCCA [CT]^GGCC[GA]
EcoRII 36 CCTGG ^CC[AT]GG
EcoT14I 155 CCTTGG C^C[AT][AT]GG
MaeI 959 CTAG C^TAG
MflI 51 AGATCC [GA]^GATC[CT]
MluNI 434 TGGCCA TGG^CCA
MscI 434 TGGCCA TGG^CCA
MspA1I 379 CCGCTG C[AC]G^C[GT]G
MvaI 36 CCTGG CC^[AT]GG
NspI 533 ACATGT [GA]CATG^[CT]
PflMI 493 CCAATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 801 ATTAAT AT^TAAT
RcaI 398 TCATGA T^CATGA
SapI 294 GCTCTTC GCTCTTC(1/4)
ScrFI 36 CCTGG CC^[ACGT]GG
SmlI 47 CTCAAG C^T[CT][GA]AG
SspBI 482 TGTACA T^GTACA
StyI 155 CCTTGG C^C[AT][AT]GG
TaqI 405 TCGA T^CGA
TthHB8I 405 TCGA T^CGA
Van91I 493 CCAATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 51 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AciI 379 CCGC CCGC(-3/-1)
981 GCGG
AluI 279 AGCT AG^CT
293 AGCT
BmyI 159 GGGCTC G[AGT]GC[ACT]^C
454 GTGCAC
BsaJI 36 CCTGGG C^C[ACGT][ACGT]GG
155 CCTTGG
BsgI 382 CTGCAC GTGCAG(16/14)
320 GTGCAG
BsmAI 3 GAGAC GTCTC(1/5)
861 GAGAC
Bsp1286I 159 GGGCTC G[AGT]GC[ACT]^C
454 GTGCAC
Cfr13I 39 GGGCC G^G[ACGT]CC
502 GGCCC
DdeI 374 CTAAG C^T[ACGT]AG
965 CTAAG
DpnII 52 GATC ^GATC
355 GATC
EarI 295 CTCTTC CTCTTC(1/4)
685 GAAGAG
Ksp632I 295 CTCTTC CTCTTC(1/4)
685 GAAGAG
MboI 52 GATC ^GATC
355 GATC
NdeII 52 GATC ^GATC
355 GATC
NlaIV 160 GGCTCC GG[ACGT]^[ACGT]CC
502 GGCCCC
PleI 581 GACTC GAGTC(4/5)
650 GAGTC
Sau3AI 52 GATC ^GATC
355 GATC
Sau96I 39 GGGCC G^G[ACGT]CC
502 GGCCC
TfiI 402 GAATC G^A[AT]TC
840 GAATC
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AfaI BbvI BsiYI BslI BstF5I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeIII MboII MnlI MseI
MslI NlaIII RsaI SfaNI Tru9I
TseI Tsp45I Tsp509I TspRI