Restriction map for
pFN21AE5903
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BcnI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRI EcoRV
EcoT14I FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MaeI MaeII
MamI MfeI MluI MroI MspA1I
MspI MunI MvnI MwoI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AlwI 471 GATCC GGATC(4/5)
AocI 666 CCTAAGG CC^T[ACGT]AGG
BalI 365 TGGCCA TGG^CCA
BbsI 715 GTCTTC GAAGAC(2/6)
BclI 236 TGATCA T^GATCA
BpuAI 715 GTCTTC GAAGAC(2/6)
BsaJI 179 CCCTGG C^C[ACGT][ACGT]GG
BsmFI 162 GTCCC GGGAC(10/14)
BsmI 864 GAATGC GAATGC(1/-1)
Bsp1407I 764 TGTACA T^GTACA
BspHI 398 TCATGA T^CATGA
BspMI 774 ACCTGC ACCTGC(4/8)
BsrDI 588 CATTGC GCAATG(2/0)
BsrGI 764 TGTACA T^GTACA
BssKI 180 CCTGG ^CC[ACGT]GG
BstEII 132 GGTCACC G^GT[ACGT]ACC
BstNI 180 CCTGG CC^[AT]GG
BstPI 132 GGTCACC G^GT[ACGT]ACC
BstYI 470 AGATCC [GA]^GATC[CT]
Bsu36I 666 CCTAAGG CC^T[ACGT]AGG
DdeI 667 CTAAG C^T[ACGT]AG
DraII 160 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 358 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 365 TGGCCA [CT]^GGCC[GA]
Eco81I 666 CCTAAGG CC^T[ACGT]AGG
EcoO109I 160 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 132 GGTCACC G^GT[ACGT]ACC
EcoRII 180 CCTGG ^CC[AT]GG
EcoT22I 282 ATGCAT ATGCA^T
FbaI 236 TGATCA T^GATCA
MflI 470 AGATCC [GA]^GATC[CT]
MluNI 365 TGGCCA TGG^CCA
MscI 365 TGGCCA TGG^CCA
MvaI 180 CCTGG CC^[AT]GG
NdeI 571 CATATG CA^TATG
NsiI 282 ATGCAT ATGCA^T
PpuMI 160 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 288 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 776 CTGCAG CTGCA^G
RcaI 398 TCATGA T^CATGA
ScrFI 180 CCTGG CC^[ACGT]GG
SfcI 776 CTGCAG C^T[GA][CT]AG
SspBI 764 TGTACA T^GTACA
XcmI 146 CCAGCCCATCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 470 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
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AfaI 171 GTAC GT^AC
765 GTAC
AluI 679 AGCT AG^CT
780 AGCT
AspHI 121 GTGCTC G[AT]GC[AT]^C
660 GTGCTC
AvaII 161 GGTCC G^G[AT]CC
502 GGTCC
BbvI 778 GCAGC GCAGC(8/12)
592 GCTGC
BsaI 771 GAGACC GGTCTC(1/5)
639 GGTCTC
BsgI 558 CTGCAC GTGCAG(16/14)
320 GTGCAG
BsiHKAI 121 GTGCTC G[AT]GC[AT]^C
660 GTGCTC
BsmAI 771 GAGAC GTCTC(1/5)
640 GTCTC
BsrI 156 ACTGG ACTGG(1/-1)
405 CCAGT
Cfr13I 161 GGTCC G^G[ACGT]CC
502 GGTCC
EarI 475 CTCTTC CTCTTC(1/4)
583 CTCTTC
Fnu4HI 592 GCTGC GC^[ACGT]GC
778 GCAGC
HphI 919 GGTGA GGTGA(8/7)
134 TCACC
ItaI 592 GCTGC GC^[ACGT]GC
778 GCAGC
Ksp632I 475 CTCTTC CTCTTC(1/4)
583 CTCTTC
MaeIII 133 GTCAC ^GT[ACGT]AC
920 GTGAC
NlaIV 160 GGGTCC GG[ACGT]^[ACGT]CC
161 GGTCCC
PflMI 357 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
570 CCATATGCTGG
PleI 288 GACTC GAGTC(4/5)
672 GACTC
RsaI 171 GTAC GT^AC
765 GTAC
Sau96I 161 GGTCC G^G[ACGT]CC
502 GGTCC
SmlI 290 CTCAAG C^T[CT][GA]AG
858 CTTGAG
TfiI 51 GAATC G^A[AT]TC
605 GATTC
TseI 592 GCTGC G^C[AT]GC
778 GCAGC
Tsp45I 133 GTCAC ^GT[GC]AC
920 GTGAC
Tsp509I 488 AATT ^AATT
893 AATT
Van91I 357 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
570 CCATATGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
BmyI BsiYI BslI Bsp1286I BstF5I
Cac8I DpnII FokI HaeIII HinfI
MboI MboII MnlI MseI MslI
NdeII NlaIII Sau3AI SfaNI Tru9I
TspRI