Restriction map for
pFN21AE5904
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# NO CUT ENZYMES ( 179 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BcgI BcnI BfaI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I Cac8I CelII Cfr10I
ClaI CpoI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRV
EcoT22I FauI FseI FspI HaeII
HapII HgaI Hin1I HincII HindII
HpaI HpaII HphI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MroI MslI MspA1I MspI
MunI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 850 GTCTAC GT^[AC][GT]AC
AcsI 25 GAATTC [GA]^AATT[CT]
AlwNI 151 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 25 GAATTC [GA]^AATT[CT]
AspHI 159 GAGCTC G[AT]GC[AT]^C
AvaII 828 GGTCC G^G[AT]CC
BalI 365 TGGCCA TGG^CCA
BanII 159 GAGCTC G[GA]GC[CT]^C
BbvCI 868 GCTGAGG CCTCAGC(-5/-2)
BbvI 489 GCTGC GCAGC(8/12)
BciVI 640 GTATCC GTATCC(6/5)
BclI 650 TGATCA T^GATCA
BglII 661 AGATCT A^GATCT
BsaI 580 GGTCTC GGTCTC(1/5)
BsiHKAI 159 GAGCTC G[AT]GC[AT]^C
BsmAI 581 GTCTC GTCTC(1/5)
BsmFI 829 GTCCC GGGAC(10/14)
BsmI 416 GCATTC GAATGC(1/-1)
BspMI 119 ACCTGC ACCTGC(4/8)
BstEII 132 GGTTACC G^GT[ACGT]ACC
BstF5I 330 CATCC GGATG(2/0)
BstPI 132 GGTTACC G^GT[ACGT]ACC
BstYI 661 AGATCT [GA]^GATC[CT]
CfoI 771 GCGC GCG^C
DraI 666 TTTAAA TTT^AAA
DsaI 845 CCGTGG C^C[GA][CT]GG
EaeI 365 TGGCCA [CT]^GGCC[GA]
EcoO65I 132 GGTTACC G^GT[ACGT]ACC
EcoRI 25 GAATTC G^AATTC
EcoT14I 576 CCTTGG C^C[AT][AT]GG
FbaI 650 TGATCA T^GATCA
Fnu4HI 489 GCTGC GC^[ACGT]GC
FokI 330 CATCC GGATG(9/13)
HhaI 771 GCGC GCG^C
HinP1I 771 GCGC G^CGC
HindIII 892 AAGCTT A^AGCTT
ItaI 489 GCTGC GC^[ACGT]GC
MaeII 73 ACGT A^CGT
MflI 661 AGATCT [GA]^GATC[CT]
MluNI 365 TGGCCA TGG^CCA
MscI 365 TGGCCA TGG^CCA
SacI 159 GAGCTC GAGCT^C
StyI 576 CCTTGG C^C[AT][AT]GG
TseI 489 GCTGC G^C[AT]GC
Tsp509I 26 AATT ^AATT
XhoII 661 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AfaI 171 GTAC GT^AC
537 GTAC
BmyI 159 GAGCTC G[AGT]GC[ACT]^C
748 GTGCCC
Bsp1286I 159 GAGCTC G[AGT]GC[ACT]^C
748 GTGCCC
Cfr13I 502 GGCCC G^G[ACGT]CC
828 GGTCC
HinfI 244 GATTC G^A[ACGT]TC
471 GATTC
NlaIV 502 GGCCCC GG[ACGT]^[ACGT]CC
828 GGTCCC
RsaI 171 GTAC GT^AC
537 GTAC
Sau96I 502 GGCCC G^G[ACGT]CC
828 GGTCC
SmlI 290 CTCAAG C^T[CT][GA]AG
657 CTTGAG
TfiI 244 GATTC G^A[AT]TC
471 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI BsaJI BsiYI BslI BssKI
BstNI DdeI DpnII EcoRII HaeIII
MaeIII MboI MboII MnlI MseI
MvaI NdeII NlaIII Sau3AI ScrFI
Tru9I Tsp45I TspRI