Restriction map for
pFN21AE5464
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaJI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmFI BsoBI BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRI EcoRV EcoT14I EcoT22I
FauI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MspA1I MspI MunI
MvnI MwoI NaeI NarI NciI
NcoI NgoMIV NheI NlaIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI TaqI Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AlwNI 803 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 121 GTGCTC G[AT]GC[AT]^C
AvaII 502 GGTCC G^G[AT]CC
BalI 365 TGGCCA TGG^CCA
BmyI 121 GTGCTC G[AGT]GC[ACT]^C
BpmI 776 CTCCAG CTGGAG(16/14)
BsgI 558 CTGCAC GTGCAG(16/14)
BsiHKAI 121 GTGCTC G[AT]GC[AT]^C
BsmI 864 GAATGC GAATGC(1/-1)
Bsp1286I 121 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 764 TGTACA T^GTACA
BspHI 398 TCATGA T^CATGA
BsrDI 588 CATTGC GCAATG(2/0)
BsrGI 764 TGTACA T^GTACA
BstEII 132 GGTCACC G^GT[ACGT]ACC
BstPI 132 GGTCACC G^GT[ACGT]ACC
Cfr13I 502 GGTCC G^G[ACGT]CC
DraIII 358 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 365 TGGCCA [CT]^GGCC[GA]
EcoO65I 132 GGTCACC G^GT[ACGT]ACC
HphI 134 TCACC GGTGA(8/7)
MluNI 365 TGGCCA TGG^CCA
MscI 365 TGGCCA TGG^CCA
NdeI 609 CATATG CA^TATG
NspI 408 GCATGT [GA]CATG^[CT]
PflMI 357 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 288 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 398 TCATGA T^CATGA
Sau96I 502 GGTCC G^G[ACGT]CC
SfaNI 202 GATGC GCATC(5/9)
SspBI 764 TGTACA T^GTACA
Van91I 357 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AlwI 161 GATCC GGATC(4/5)
471 GATCC
BbvI 405 GCAGC GCAGC(8/12)
592 GCTGC
BclI 236 TGATCA T^GATCA
545 TGATCA
BfaI 424 CTAG C^TAG
577 CTAG
BsaI 771 GAGACC GGTCTC(1/5)
639 GGTCTC
BssKI 180 CCTGG ^CC[ACGT]GG
636 CCTGG
BstF5I 330 CATCC GGATG(2/0)
280 GGATG
BstNI 180 CCTGG CC^[AT]GG
636 CCTGG
BstYI 160 AGATCC [GA]^GATC[CT]
470 AGATCC
EarI 475 CTCTTC CTCTTC(1/4)
583 CTCTTC
EcoRII 180 CCTGG ^CC[AT]GG
636 CCTGG
FbaI 236 TGATCA T^GATCA
545 TGATCA
Fnu4HI 405 GCAGC GC^[ACGT]GC
592 GCTGC
FokI 330 CATCC GGATG(9/13)
280 GGATG
ItaI 405 GCAGC GC^[ACGT]GC
592 GCTGC
Ksp632I 475 CTCTTC CTCTTC(1/4)
583 CTCTTC
MaeI 424 CTAG C^TAG
577 CTAG
MaeIII 19 GTGAC ^GT[ACGT]AC
133 GTCAC
MflI 160 AGATCC [GA]^GATC[CT]
470 AGATCC
MslI 393 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
756 CATATTTGTG
MvaI 180 CCTGG CC^[AT]GG
636 CCTGG
PleI 288 GACTC GAGTC(4/5)
581 GACTC
ScrFI 180 CCTGG CC^[ACGT]GG
636 CCTGG
SmlI 290 CTCAAG C^T[CT][GA]AG
858 CTTGAG
TfiI 51 GAATC G^A[AT]TC
244 GATTC
TseI 405 GCAGC G^C[AT]GC
592 GCTGC
Tsp45I 19 GTGAC ^GT[GC]AC
133 GTCAC
Tsp509I 488 AATT ^AATT
893 AATT
XhoII 160 AGATCC [GA]^GATC[CT]
470 AGATCC
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AfaI AluI BsiYI BslI BsmAI
Cac8I DdeI DpnII HaeIII HinfI
MboI MboII MnlI MseI NdeII
NlaIII RsaI Sau3AI Tru9I TspRI