Restriction map for
pFN21AE5462
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FauI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeII
MamI MfeI MflI MluI MroI
MspA1I MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 864 AAATTC [GA]^AATT[CT]
AflIII 533 ACATGT A^C[GA][CT]GT
ApoI 864 AAATTC [GA]^AATT[CT]
AspHI 304 GAGCAC G[AT]GC[AT]^C
AspI 25 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 782 GGACC G^G[AT]CC
BbsI 736 GTCTTC GAAGAC(2/6)
BbvI 423 GCTGC GCAGC(8/12)
BciVI 537 GTATCC GTATCC(6/5)
BclI 516 TGATCA T^GATCA
BpmI 897 CTGGAG CTGGAG(16/14)
BpuAI 736 GTCTTC GAAGAC(2/6)
BsiHKAI 304 GAGCAC G[AT]GC[AT]^C
BspHI 398 TCATGA T^CATGA
BspLU11I 533 ACATGT A^CATGT
BstF5I 218 CATCC GGATG(2/0)
BstXI 570 CCATGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 517 GATC ^GATC
DraI 666 TTTAAA TTT^AAA
DraII 781 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 107 CCATGG C^C[GA][CT]GG
EcoO109I 781 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 202 GATATC GAT^ATC
FbaI 516 TGATCA T^GATCA
Fnu4HI 423 GCTGC GC^[ACGT]GC
FokI 218 CATCC GGATG(9/13)
HinfI 51 GAATC G^A[ACGT]TC
ItaI 423 GCTGC GC^[ACGT]GC
MboI 517 GATC ^GATC
MwoI 769 GCTAGACCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 107 CCATGG C^CATGG
NdeII 517 GATC ^GATC
NspI 533 ACATGT [GA]CATG^[CT]
PflFI 25 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 493 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 781 AGGACCT [GA]G^G[AT]CC[CT]
PstI 711 CTGCAG CTGCA^G
RcaI 398 TCATGA T^CATGA
SapI 90 GCTCTTC GCTCTTC(1/4)
Sau3AI 517 GATC ^GATC
ScaI 69 AGTACT AGT^ACT
SfcI 711 CTGCAG C^T[GA][CT]AG
TaqI 302 TCGA T^CGA
TfiI 51 GAATC G^A[AT]TC
TseI 423 GCTGC G^C[AT]GC
Tth111I 25 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 302 TCGA T^CGA
Van91I 493 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 596 CCAATGGAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AluI 293 AGCT AG^CT
893 AGCT
BalI 365 TGGCCA TGG^CCA
593 TGGCCA
BanII 159 GGGCTC G[GA]GC[CT]^C
487 GGGCTC
BfaI 770 CTAG C^TAG
779 CTAG
BsaI 151 GAGACC GGTCTC(1/5)
905 GAGACC
BsgI 454 CTGCAC GTGCAG(16/14)
558 CTGCAC
BsiYI 493 CCATTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
582 CCTCTTAGTGG
BslI 493 CCATTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
582 CCTCTTAGTGG
BsrI 552 ACTGG ACTGG(1/-1)
606 ACTGG
BssKI 156 CCTGG ^CC[ACGT]GG
437 CCTGG
BstNI 156 CCTGG CC^[AT]GG
437 CCTGG
Cac8I 184 GCTGGC GC[ACGT]^[ACGT]GC
610 GCTTGC
EaeI 365 TGGCCA [CT]^GGCC[GA]
593 TGGCCA
EarI 91 CTCTTC CTCTTC(1/4)
902 GAAGAG
EcoRII 156 CCTGG ^CC[AT]GG
437 CCTGG
EcoT14I 7 CCAAGG C^C[AT][AT]GG
107 CCATGG
HphI 342 GGTGA GGTGA(8/7)
163 TCACC
Ksp632I 91 CTCTTC CTCTTC(1/4)
902 GAAGAG
MaeI 770 CTAG C^TAG
779 CTAG
MluNI 365 TGGCCA TGG^CCA
593 TGGCCA
MscI 365 TGGCCA TGG^CCA
593 TGGCCA
MseI 667 TTAA T^TAA
847 TTAA
MvaI 156 CCTGG CC^[AT]GG
437 CCTGG
ScrFI 156 CCTGG CC^[ACGT]GG
437 CCTGG
StyI 7 CCAAGG C^C[AT][AT]GG
107 CCATGG
Tru9I 667 TTAA T^TAA
847 TTAA
Tsp509I 262 AATT ^AATT
865 AATT
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AfaI BmyI BsaJI BsmAI Bsp1286I
BspMI Cfr13I DdeI HaeIII MaeIII
MboII MnlI MslI NlaIII NlaIV
RsaI Sau96I Tsp45I TspRI